7. Conclusion
Metabolic profiling by FT-ICR-MS and ESI-Q-TOF-MS is an effective method to capture a rough image of metabolism. It should be performed after the accuracy is confirmed by using the authentic sample after an elaborate calibration is done, when an individual metabolite is identified from the accurate mass for FT-ICR-MS. Moreover, when ESI-Q-TOF-MS is used, the CID spectrum should also be measured, and compared with that of the authentic sample.
Keywords
- Molecular Formula
- Differential Spectrum
- Hepes Buffer Solution
- Electrospray Ionization Quadrupole
- Intracellular Element
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Aharoni A, Ric De Vos CH, Verhoeven HA, Maliepaard CA, Kruppa G, Bino R, Goodenowe DB (2002) Nontargeted metabolome analysis by use of Fourier transform ion cyclotron mass spectrometry. J Integr Biol 6:217–234
Maharjan R, Ferenci T (2003) Global metabolite analysis: the influence of extraction methodology on metabolome profiles of Escherichia coli. Anal Biochem 313:145–154
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer-Verlag Tokyo
About this chapter
Cite this chapter
Hirayama, K. (2005). Metabolic Profiling by Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Electrospray Ionization Quadrupole Time-Of-Flight Mass Spectrometry (ESI-Q-TOF-MS). In: Tomita, M., Nishioka, T. (eds) Metabolomics. Springer, Tokyo. https://doi.org/10.1007/4-431-28055-3_6
Download citation
DOI: https://doi.org/10.1007/4-431-28055-3_6
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-25121-7
Online ISBN: 978-4-431-28055-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)