Summary
With regards to the therapy for end-stage failing hearts, the heart transplantation is the only effective treatment but is still limited by donor shortage and chronic rejection. Therefore, a novel strategy for myocardial regeneration is desired. Cell transplantation, a new approach for restoring impaired hearts still has several problems. Especially, autologous skeletal myoblasts (Skm) transplantation (Ctx) by injection has been clinically applied. However, its optimal effect and stable delivery methods are undetermined. Tissue implantation provides stable cell delivery with maintained inter-cellular communication and extracellular matrix. We hypothesize that the Skm tissue cardiomyoplasty might be more advantageous in regenerating the impaired heart. LAD ligated hearts (2 weeks) received Skm Ctx either by injection (MI; n=9) or by implanting two engineered monolayer myoblasts sheets (MS; n=9) or non-cellular therapy (Control=C; n=10). After 8 weeks, higher improvement of ejection fraction (%) was measured in MS compared to that of the other groups. Histological comparison revealed greater cellularity and abundant widespread neo-capillaries with a noticeably uniformly thickened wall in MS only. These results demonstrated that engineered skeletal myoblasts sheets regenerated the impaired myocardium, suggesting a promising therapy for severe heart failure.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kofidis T, Akhyari P, Haverich A, et al. In Vitro engineering of heart muscle: Artificial myocardial tissue. J Thorac Cardiovasc Surg. 2002; 124(1):63–9.
Kollet O, Shivtiel S, Lapidot T, et al. HGF, SDF-1 and MMP-9 are involved in stress-induced human CD34+ stem cell recruitment to the liver. J Clin Invest. 2003; 112(2): 160–9
Kushida A, Yamato M, Okano T, et al. Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix from temperature-responsive culture surfaces. J Biomed Mater Res. 1999; 45:355–362
Lanza RP, Langer R, Vacatni J. Principles of tissue engineering (book reference).2003; 13–15
Leor J, Etzion SA, Dar A, et al. Bioengineered cardiac Grafts. A new approach to repair the infracted Myocardium? Circulation 2000; 102[suppl III] III-56–III-61
Miyagawa et al. Impaired myocardium regeneration with bioengineered contractile cardiomyocyte sheets-a novel concept of tissue cardiomyoplasty for repairing damaged myocardium 2004
Okano T, Yamada N, Sakai H, Sakurai Y, et al. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly (N-isopropylacrylamide) J Biomed Mater Res. 1993;27(10): 1243–51
Ozawa T, Mickle DA, Ozawa S, Li RK, et al. Optimal Biomaterial for Creation of Autologous Cardiac Grafts. Circulation 2002; 106[suppl I]: I-176–I-182
Pagani FD, Aretz TH, Aaronson KD, et al. Autologous skeletal myoblasts transplanted to ischemic-damaged myocardium in humans. J American Coll Cardiol 2003; 41:879–88
Pouzet B, Vilquin JT, Menasche P, et al. Factors affecting functional outcome after autologous skeletal myoblast transplantation. Ann Thorac Surg. 2001; 71:844–851
Reinecke H, MacDonald GH, Murry CE, et al. Electromechanical coupling between skeletal and cardiac, muscle: implications for the infarct repair. J Cell Biol. 2000; 149:731–740
Reinecke H, Poppa V, Murry CE. Skeletal muscle stem cells do not transdifferentiate into cardiomyocytes after cardiac grafting. J Mol Cell Cardiol 2002; 34:241–249
Shimizu T, Umezu M, Okano T, et al. Fabrication of Pulsatile cardiac Tissue Grafts Using a Novel 3-Dimensional Cell Sheet Manipulation Technique and Temperature-Responsive Cell Culture Surfaces. Circulation Research 2002; 90:e40–e48
Shimizu T, Yamato M, Okano T, et al. Cell sheet engineering for myocardial tissue reconstruction Biomaterials 2003;24(13):2309–16
Tatsumi R, Anderson JE, Allen RE, et al. HGF/SF is present in normal adult skeletal muscle and is capable of activating satellite cells. Dev Biol. 1998; 194: 114–128
Zimmermann WH, Didie M, Eschenhagen T, et al. Cardiac Grafting of Engineered Heart Tissue in Syngenic Rats. Circulation 2002; 106[suppl I]: I-151–I-157
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer-Verlag Tokyo
About this chapter
Cite this chapter
Sawa, Y., Memon, I., Matsuda, H. (2005). Myocardial Regeneration Therapy with Tissue Implantation of Autologous Myoblast Sheets for Severe Impaired Heart Failure. In: Mori, H., Matsuda, H. (eds) Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches. Springer, Tokyo. https://doi.org/10.1007/4-431-27378-6_5
Download citation
DOI: https://doi.org/10.1007/4-431-27378-6_5
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-23925-3
Online ISBN: 978-4-431-27378-3
eBook Packages: MedicineMedicine (R0)