Abstract
We have used synchrotron X-ray scattering from solutions to study the assembly of biopolymers. The principles of the method are reviewed, and applications are given for the example of microtubule self-assembly. We show how different structural intermediates of the reaction can be distinguished. Assembly can take the form of a first-order approach to a steady state, but non-equilibrium phenomena are also observed. An example is the unique behavior of synchronized oscillations in microtubule assembly.
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© 1989 Springer-Verlag
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Mandelkow, E., Lange, G., Mandelkow, EM. (1989). Applications of synchrotron radiation to the study of biopolymers in solution: Time-resolved X-ray scattering of microtubule self-assembly and oscillations. In: Mandelkow, E. (eds) Synchrotron Radiation in Chemistry and Biology III. Topics in Current Chemistry, vol 151. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3540512012_11
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DOI: https://doi.org/10.1007/3540512012_11
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