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Embryogenesis in Citrus tissue cultures

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Advances in Biochemical Engineering, Volume 16

Part of the book series: Advances in Biochemical Engineering ((ABE,volume 16))

Abstract

The morphogenetic potential of citrus tissue in vitro has been realized to full extent in explants from the somatic cells of the nucellus. the role of apomixis and polyembryony in citrus evolution, propagation and breeding has been evaluated. while nucellar tissue has given rise to embryos in vitro in polyembryonic, and in rare cases, monoembryonic cultivars, it also produces an embryogenic callus in many citrus species and cultivars. conditions and factors for realizing its embryogenetic potential have been described. lowering the auxin status, anti-auxins, irradiation and the use of galac-tose-yielding sugars have enhanced embryogenesis. protoplast cultures have been established from the nucellar callus, also giving rise to plants via newly formed callus. callus has maintained stability under prolonged subculture and remained diploid. selection of callus lines tolerant to diverse stresses (salinity, 2,4-d) has been initiated and stress-tolerant, embryogenic callus lines have been established. the significance of these culture methods for mutation breeding is discussed.

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Abbreviations

MS:

Murashige and Skoog medium

IAA:

3-indole acetic acid

ABA:

abscissic acid

GA3:

gibberellic acid

5-HNB:

2-hydroxy-5-nitro benzyl bromide

NAA:

α naphthyl acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

IPA:

N6-(Δ-isopentyl) adenine

AG:

8-azaguanine

CCC:

2-chloroethyl trimethylammonium chloride

Alar:

succinic acid 2,2-methylhydrazide

Ethephon:

2-chloroethyl phosphonic acid

UDP:

uridine diphosphate

CM:

coconut milk

YE:

yeast extract

CH:

casein hydrolysate

ME:

malt extract

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  1. Division of Fruit Breeding and Genetics, Institute of Horticulture, A.R.O. The Volcani Center, P.O. Box 6, Bet Dagan, Israel

    P. Spiegel-Roy & J. Kochba

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  1. P. Spiegel-Roy
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  2. J. Kochba
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Spiegel-Roy, P., Kochba, J. (1980). Embryogenesis in Citrus tissue cultures. In: Fiechter, A. (eds) Advances in Biochemical Engineering, Volume 16. Advances in Biochemical Engineering, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3540098070_7

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