Abstract
A new plant cell image segmentation algorithm is presented in this paper. It is based on the morphological analysis of the cell shapes and on gradient information. The difficulty of the segmentation of plant cells lies in the complex shapes of the cells and their overlapping, often present due to recent cellular division. The algorithm presented tries to imitate the human procedure for segmenting overlapping and touching particles. It analyzes concavities in the shape of a group of cells as well as the existence of a coherent surface for the segmentation. The algorithm can be divided in two main parts, firstly a simple method for finding dominant concave points in shapes is introduced and secondly several parameters between concave points are calculated as a criterion for segmentation. It has been also shown that the algorithm produces good results when the output is applied as a marker for the morphological watershed algorithm. Results will be presented in real images of a cell suspension culture to show the validity of the chosen approach.
This work is supported by Swiss National Science Foundation (FNRS) grant N. 3136561.92
Chapter PDF
Similar content being viewed by others
References
S. Beucher and F. Meyer. Morphological segmentation. Journal of Visual Communication and Image Representation, 1:21–46, 1990.
P. Brigger. Morphological plant cell analysis. In J. Serra and P. Salembier, editors, Mathematical Morphology and its Applications to Signal Processing, pages 101–106, Barcelona, May 1993.
R. Duda and P. Hart. Use of the hough transformation to detect lines and curves in pictures. Communications of the ACM, 15:11–15, 1972.
F. Meyer. Cytologie quantitative et morphologie mathématique. PhD thesis, Ecole Nationale Supérieure des Mines de Paris, Fontainebleau, 1979.
A.K. Jain, S.P. Smith, and E. Backer. Segmentation of muscle cell pictures: a preliminary study. IEEE Transactions on Pattern Analysis and Machine Intelligence, 3:232–242, 1980.
A. Lerch and D. Chetverikov. Correction of line drawings for image segmentation in leather industry. In Proc. 11th Int. Conf. on Pattern Recognition, volume 1, pages 420–424, The Hague, 1992.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1995 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Fernàndez, G., Kunt, M., Zrÿd, J.P. (1995). A new plant cell image segmentation algorithm. In: Braccini, C., DeFloriani, L., Vernazza, G. (eds) Image Analysis and Processing. ICIAP 1995. Lecture Notes in Computer Science, vol 974. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-60298-4_263
Download citation
DOI: https://doi.org/10.1007/3-540-60298-4_263
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-60298-9
Online ISBN: 978-3-540-44787-0
eBook Packages: Springer Book Archive