Abstract
The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono) xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 ± 0.4)ml/gand (77.6 ± 0.5) ml/g, respectively. Sedimentation coefficients, s 020 w, were calculated to be (1.72 ± 0.06) S and (1.77 ± 0.06) S and (weight-average) molecular weights, M w, from sedimentation equilibrium were found to be (37000 ± 1500) g/mol and (40000 ± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change.
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© 1999 Springer-Verlag
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Morris, G.A., Ebringerova, A., Harding, S.E., Hromadkova, Z. (1999). UV tagging leaves the structural integrity of an arabino-(4-O-methylglucurono)xylan polysaccharide unaffected. In: Cölfen, H. (eds) Analytical Ultracentrifugation V. Progress in Colloid and Polymer Science, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-48703-4_29
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DOI: https://doi.org/10.1007/3-540-48703-4_29
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