Abstract
The subfragment-1 (S1) of the contractile protein myosin is the postulated site of energy transduction in muscle. It is currently considered that a large conformational change in this moeity, which forms ‘cross-bridges’ between the thick (myosin based) and thin (actin based) filaments, is the mechanical driving force which leads to mutual sliding of the two types of filaments, and hence to contraction.
We have studied the possibility that S1 from skeletal myosin can in free solution (and in the absence of actin) be induced to undergo related changes in conformation, in the presence of a range of effectors whose action mimics stages of the contractile cycle. Analysis of the g(s *) profiles of S1 under these conditions, displayed after force-fitting of the known monomer mass as conformation spectra (CON-SPECs-[7]), shows that changes of the type associated with the contractile cycle are readily detected. There is full qualitative and extensive quantitative agreement between the magnitude of the changes seen and those predicted on the basis of hydrodynamic bead modeling and high resolution electron microscopy. Results from recently published X-ray crystallography of smooth muscle S1 [8] are also in general agreement with our findings.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Huxley AF, Niedergerke R (1954) Nature 173: 973
Huxley AF, Simmons RM (1971) Nature 233: 533
Finer JT, Simmons RM, Spudich JA (1994) 368: 113
Rayment I, Rypiewski WR, Schmidt-Base K, Smith R, Tomchick DR, Benning MW, Winkelmann DA, Wesenberg G, Holden HM (1993) Science 261: 50
Holmes KC (1997) Curr Biol 7: R1 12
Wakabayashi K, Tokunaga M, Kohno I, Sugimoto Y, Hamaka T, Tukezawa Y, Wakabayashi T, Amemya Y (1992) Science 258: 443
Errington N, Rowe AJ (1999) Biophys Chem (in press)
Goodno GC (1982) Methods Enzymol 85: 116
Dominguez R, Freyson Y, Trybus KM, Cohen C (1998) Cell 94: 559
Persechini AJ, Rowe AJ (1984) J Mol Biol 172: 23
Margossian SS, Lowey S (1982) Methods Enzymol 85: 63
Stafford WF (1992) Anal Biochem 203: 295
Willison JHM, Rowe AJ (1980) In: Glauert A (ed) Practical methods in electron microscopy, vol. 8. North-Holland, Amsterdam
Lymm RW, Taylor EW (1971) Biochemistry 10: 4617
Jontes JD et al (1995) Nature 378: 751
Whittaker M et al (1995) Nature 378: 748
Author information
Authors and Affiliations
Corresponding author
Editor information
Rights and permissions
Copyright information
© 1999 Springer-Verlag
About this paper
Cite this paper
Bayliss, R.I., Errington, N., Byron, O., Svensson, A., Rowe, A. (1999). A conformation spectrum analysis of the morphological states of myosin S1 in the presence of effectors. In: Cölfen, H. (eds) Analytical Ultracentrifugation V. Progress in Colloid and Polymer Science, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-48703-4_22
Download citation
DOI: https://doi.org/10.1007/3-540-48703-4_22
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66175-7
Online ISBN: 978-3-540-48703-6
eBook Packages: Springer Book Archive