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Calculation of structural parameters from hydrodynamic data

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Analytical Ultracentrifugation V

Part of the book series: Progress in Colloid and Polymer Science ((PROGCOLLOID,volume 113))

Abstract

Biopolymers of simple overall shape (nonconjugated and conjugated proteins, viruses, ribonucleic acids) can be modeled by whole-body approaches, approximating their shape by prolate/ oblate ellipsoids of revolution or spheres. A sophisticated rearrangement of the theoretical formalism already applied for the prediction of hydrodynamic data from solution scattering or crystal data allows the inverse procedure, the prediction of structural parameters on the basis of hydrodynamic data. Sedimentation and diffusion coefficients, in addition to molar masses, partial specific volumes and values for hydration, are used to predict structural parameters typical of small-angle X-ray scattering studies (radii of gyration, volumes, surface-to-volume ratios and surface areas), together with estimates of axial ratios. For particles of simple shape such as globular proteins, fair agreement between observed and predicted values was achieved. Far-reaching conformity between experiments and calculations was also obtained for the prediction of subtle ligand-induced shape changes. A critical assessment of errors reveals the validity of anticipations of structural parameters from hydro-dynamic data. The accuracy to be obtained, however, turns out to be less than for the reverse procedure. Use of viscosity data for parameter predictions cannot be recommended.

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Authors and Affiliations

  1. Institute of Biophysics and Physical Biochemistry, University of Regensburg, Universitätsstrasse 31, D-93040, Regensburg, Germany

    H. Durchschlag

  2. Institute of Physical Chemistry, University of Graz, Heinrichstrasse 28, A-8010, Graz, Austria

    P. Zipper

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  1. H. Durchschlag
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  2. P. Zipper
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Correspondence to H. Durchschlag .

Editor information

Helmut Cölfen

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© 1999 Springer-Verlag

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Durchschlag, H., Zipper, P. (1999). Calculation of structural parameters from hydrodynamic data. In: Cölfen, H. (eds) Analytical Ultracentrifugation V. Progress in Colloid and Polymer Science, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-48703-4_14

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  • DOI: https://doi.org/10.1007/3-540-48703-4_14

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-66175-7

  • Online ISBN: 978-3-540-48703-6

  • eBook Packages: Springer Book Archive

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