Differential scanning calorimetry and structural studies of the sol-gel transition of gellan gum in water

  • Y. IzumiEmail author
  • S. Saito
  • K. Soma
Conference paper
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 114)


The sol-gel transition of gellan gum aqueous solution in the absence of added salts has been studied by differential scanning calorimetry (DSC), mechanical methods, wide-angle X-ray diffraction (WAXD), and small-angle X-ray scattering using synchrotron radiation (SR-SAXS). DSC data show that the endothermic enthalpies have a maximum at about 4% gellan gum concentration and almost disappear at a concentration beyond about 8%, whereas the exothermic enthalpies increase with the concentration of gellan gum. The endothermic peak temperatures are almost coincident with the exothermic temperatures at gellan gum concentrations below about 4%. In a concentration range between about 5 and 7%, two endothermic peaks were observed.

At gellan gum concentrations above about 8%, no endothermic peak was observed in the DSC heating curves, suggesting a splitting into many endothermic peaks. Two transition curves determined by DSC and test-tube tilting methods cross over at about 5 wt% gellan gum concentration. The solution was transparent below about 5% gellan gum concentration, while it was cloudy at higher concentration and the cloudiness increased on storing overnight at around 30 °C. WAXD and SR-SAXS data indicated that some peaks indicating an ordered structure were observed in the cloudy gels. The data on the cloudy gels were characterized by peaks at three scattering vectors which are in a simple ratio of 1:2:3, suggesting that the junction zones in these gels are formed by lamellar structures. These peaks shifted to a lower scattering vector as the content of added ions in the gellan gum sample was increased, indicating larger lamellae.

The molecular origin of the multiple endothermic peaks observed in the DSC heating curves for gellan gum solutions at higher concentration is the melting of these lamellae.

Key words

Gellan gum Differential scanning calorimetry Small-angle X-ray scattering Structure of gels Lamellae 


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  1. 1.
    Kang KS, Veeder GT (1982) US Patent 4, 326, 053Google Scholar
  2. 2.
    O’Neill MA, Selvendran R, Morris VJ (1983) Carbohydr Res 124: 123CrossRefGoogle Scholar
  3. 3.
    Jansson P, Lindberg Sandford PA (1983) Carbohydr Res 124: 135CrossRefGoogle Scholar
  4. 4.
    Dentini M, Coviello T, Burchard W, Crescenzi V (1988) Macromolecules 21: 3312CrossRefGoogle Scholar
  5. 5.
    Gunning AP, Morris VJ (1990) Int J Biol Macromol 12: 338CrossRefGoogle Scholar
  6. 6.
    Robinson G, Manning CE, Morris ER (1991) In: Dickinson E (ed) Food polymers, gels and colloids. The Royal Society of Chemistry, Cambridge, pp 22–33Google Scholar
  7. 7.(a)
    Miyoshi E, Takaya T, Nishinari K (1994) Food Hydrocolloids 8: 505Google Scholar
  8. 7.(b)
    Miyoshi E, Takaya T, Nishinari K (1994) Food Hydrocolloids 8: 529CrossRefGoogle Scholar
  9. 8.
    Gunning AP, Kirby AR, Ridout AR, Brownsey GJ, Morris VJ (1996) Macromolecules 29: 6791CrossRefGoogle Scholar
  10. 9.
    Miyoshi E, Takaya T, Nishinari K (1996) Carbohydr Polym 30: 109CrossRefGoogle Scholar
  11. 10.(a)
    Ogawa E (1996) Macromolecules 29: 5178CrossRefGoogle Scholar
  12. 10.(b)
    Ogawa E (1996) Carbohydr Polym 30: 145CrossRefGoogle Scholar
  13. 11.
    Nishinari K (1997) Colloid Polym Sci 275: 1093CrossRefGoogle Scholar
  14. 12.
    Yuguchi Y, Mimura M, Urakawa H, Kitamura S, Ohno S, Kajiwara Carbohydr Polym 30: 83Google Scholar
  15. 13.
    Izumi Y, Kikuta N, Sakai K, Takezawa H (1996) Carbohydr Polym 30: 121CrossRefGoogle Scholar
  16. 14.
    Ogawa E (1999) Progr Colloid Polym Sci 114: 8–14CrossRefGoogle Scholar
  17. 15.
    Mica A, Kubota K, Nakamura K (1998)Google Scholar
  18. 16.
    Takahashi A, Sakai M, Kato T (1980) Polym J 12: 335CrossRefGoogle Scholar
  19. 17.
    Tan HM, Moet A, Hiltner A, Baer E (1983) Macromolecules 16: 28CrossRefGoogle Scholar
  20. 18.
    Izumi Y, Takezawa H, Kikuta N, Uemura S, Tsutsumi A (1998) Macromolecules 31: 430CrossRefGoogle Scholar
  21. 19.
    Ueki T, Hiragi Y, Kataoka M, Inoko Y, Amemiya Y, Izumi Y, Tagawa H, Muroga Y (1985) Biophys Chem 23: 115CrossRefGoogle Scholar
  22. 20.
    Hosemann R, Baguchi SN (1962) Direct analysis of diffraction by matter. North Holland, Amsterdam, pp 408–415Google Scholar

Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  1. 1.Graduate Program of Human Sensing and Functional Sensor Engineering Graduate School of Science and EngineeringYamagata UniversityYamagataJapan

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