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Characterization of gellan gum in aqueous NaCl solution

  • R. Takahashi
  • M. Akutu
  • K. KubotaEmail author
  • K. Nakamura
Conference paper
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 114)

Abstract

The characterization of gellan gum in 25 mM NaCl solution at 25.0 and 40.0 °C was achieved by using static and dynamic light scattering techniques focusing on the molecular-weight distribution. The molecular weights were 21.7 × 104 at 25.0 °C (the ordered state) and 9.47 × 104 at 40.0 °C (the disordered state), and the ratio close to 2 supported the scheme of the conformational transition of gellan gum between a double helix and a single -stranded coil. The molecular-weight distribution of the gellan gum sample was examined by the Laplace inversion method of the correlation function obtained by dynamic light scattering and by the wormlike chain model with a persistence length of 98 nm at 25.0 °C and 17 nm at 40.0 °C. The distribution obtained reproduced the molecular properties of gellan gum in 25 mM NaCl reasonably well. The present gellan gum sample has a large polydispersity of molecular weight. The chain stiffness changes greatly with temperature, and the gellan molecule behaves as a stiff chain even in the disordered state of a single-stranded chain. The substantial stiffness in the single-stranded state makes double-helix formation easy.

Key words

Gellan gum Light scattering Molecular-weight distribution Wormlike chain Stiffness 

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Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • R. Takahashi
    • 1
  • M. Akutu
    • 1
  • K. Kubota
    • 1
    Email author
  • K. Nakamura
    • 2
  1. 1.Department of Biological and Chemical Engineering Faculty of EngineeringGunma UniversityGunmaJapan
  2. 2.Department of Food Science Faculty of Dairy ScienceRakuno Gakuen UniversityHokkaidoJapan

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