A supramolecular construction kit based on surface-layer proteins

  • Uwe B. SleytrEmail author
  • Dietmar Pum
  • Erika Györvary
  • Bernhard Schuster
  • Seta Küpcü
  • Margit Sára
Conference paper
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 121)


Crystalline monomolecular cell surface layers (S layers) represent one of the most commonly observed cell envelope components of prokaryotic organisms (bacteria and archaea). Owing to their high degree of structural regularity, S layers represent interesting model systems for studies on structure, genetics, functions and dynamic aspects of assembly of supramolecular structures. One of the key features of isolated S-layer subunits is their capability to assemble into monomolecular arrays either in suspension, at liquid-air interfaces, on lipid films, on liposomes and on solid supports (e.g. polymers, metals, silicon wafers). S layers have already found a broad spectrum of applications, such as patterning elements in molecular nanotechnology, nanobio-technology and biomimetics.

Key words

Crystalline surface layers Surface layers Nanotechnology Biomimetics 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Sleytr UB, Messner P, Pum D, Sára M (1999) Angew Chem Int Ed Engl 38:1034CrossRefGoogle Scholar
  2. 2.
    Sleytr UB, Messner P, Pum D, Sára M (1996) Crystalline bacterial cell surface proteins. Landes, AustinGoogle Scholar
  3. 3.
    Sleytr UB, Messner P, Pum D, Sára M (1988) Crystalline bacterial cell surface layers. Springer, Berlin Heidelberg New YorkGoogle Scholar
  4. 4.
    Sleytr UB, Beveridge TJ (1999) Trends Microbiol 7:253–260CrossRefGoogle Scholar
  5. 5.
    Sára M, Sleytr UB (1989) Appl Microbiol Biotechnol 30:184CrossRefGoogle Scholar
  6. 6.
    Sleytr UB, Messner P (1989) In: Plattner H (ed) Electron microscopy of subcellular dynamics. CRC, Boca Raton, pp 13–31Google Scholar
  7. 7.
    Pum D, Weinhandl M, Hödl C, Sleytr UB (1993) J Bacteriol 175:2762Google Scholar
  8. 8.
    Pum D, Sleytr UB (1995) Supramol Sci 2:193CrossRefGoogle Scholar
  9. 9.
    Pum D, Sleytr UB (1994) Thin Solid Films 244:882CrossRefGoogle Scholar
  10. 10.
    Schuster B, Sleytr UB (2000) Rev Mol Biotechnol 74:233CrossRefGoogle Scholar
  11. 11.
    Küpcü S, Sára M, Sleytr UB (1995) Biochim Biophys Acta 1235:263CrossRefGoogle Scholar
  12. 12.
    Mader C, Küpcü S, Sára M, Sleytr UB (1999) Biochim Biophys Acta 1418:106CrossRefGoogle Scholar
  13. 13.
    Sleytr UB, Sára M, Pum D (2000) In: Ciferri A (ed) Supramolecular polymerisation. Dekker, New York, pp 177–213Google Scholar
  14. 14.
    Sleytr UB, Sára M, Pum D, Schuster B (2001) In: Rosoff M (ed) Nano-surface chemistry. Dekker, New York, pp 333–389Google Scholar
  15. 15.
    Sleytr UB, Sára M, Pum D, Schuster B (2001) Progr Surf Sci 68:231CrossRefGoogle Scholar
  16. 16.
    Messner P, Pum D, Sára M, Stetter KO, Sleytr UB (1986) J Bacteriol 166:1046Google Scholar
  17. 17.
    Howorka S, Sára M, Wang Y, Kuen B, Sleytr UB, Lubitz W, Bayley H (2000) J Biol Chem 275:37876CrossRefGoogle Scholar
  18. 18.
    Jarosch M, Egelseer EM, Huber C, Moll D, Mattanovich D, Sleytr UB, Sára M (2001) Microbiology 147:1353Google Scholar
  19. 19.
    Sára M, Sleytr UB (2000) J Bacteriol 182:859CrossRefGoogle Scholar
  20. 20.
    Breitwieser A, Egelseer EM, Ilk N, Moll D, Hotzy C, Sleytr UB, Sára M (2001) Protein Eng (submitted)Google Scholar
  21. 21.
    Shenton W, Pum D, Sleytr UB, Mann S (1997) Nature 389:585CrossRefGoogle Scholar
  22. 22.
    Dieluweit S, Pum D, Sleytr UB (1998) Supramol Sci 5:15CrossRefGoogle Scholar
  23. 23.
    Mertig M, Kirsch R, Pompe W, Engelhardt H (1999) Eur Phys J D 9:45CrossRefGoogle Scholar
  24. 24.
    Pum D, Stangl G, Sponer C, Fallmann W, Sleytr UB (1996) Colloids Surf B 8:157CrossRefGoogle Scholar
  25. 25.
    Sackman E (1996) Science 271:43CrossRefGoogle Scholar
  26. 26.
    Györvary E, Wetzer B, Sleytr UB, Sinner A, Offenhäusser A, Knoll W (1999) Langmuir15:1337CrossRefGoogle Scholar
  27. 27.
    Wetzer B, Pum D, Sleytr UB (1997) J Struct Biol 119:123CrossRefGoogle Scholar
  28. 28.
    Schuster B, Pum D, Braha O, Bayley H, Sleytr UB (1998) Biochim Biophys Acta Biomembr 1370:280CrossRefGoogle Scholar
  29. 29.
    Schuster B, Pum D, Sára M, Braha O, Bayley H, Sleytr UB (2001) Langmuir 17:499CrossRefGoogle Scholar
  30. 30.
    Schuster B, Pum D, Sleytr UB(1998) Biochim Biophys Acta Biomembr 1369:51CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Uwe B. Sleytr
    • 1
    Email author
  • Dietmar Pum
    • 1
  • Erika Györvary
    • 1
  • Bernhard Schuster
    • 1
  • Seta Küpcü
    • 1
  • Margit Sára
    • 1
  1. 1.Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular NanotechnologyUniversität für BodenkulturViennaAustria

Personalised recommendations