Abstract
The laser light scattering technique can be used in a nonconventional fashion to study dynamic properties of vesicles which are too small to be observed by microscopy. In fact, in suitable experimental conditions, the correlation function of the scattered light contains a contribution from bilayer fluctuations, besides the usual diffusion one. Characteristic fluctuation times have been determined for single-component phospholipid vesicles of 60 nm radius, prepared by extrusion. The addition of small amounts of a glycolipid (to 2% mole fraction), induces a significative increase in the fluctuation times (of the order of 20%) but still does not affect the diffusive motion, indicating a softening of the membrane. Being so sensitive, this technique is quite promising both for the study of membrane properties in the presence of defects and for applications to biology and pharmacology.
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References
Evans E, Rawicz W (1997) Phys Rev Lett 79:2379
Needham D, Zhelev DV (1996) In: Rosoff M (ed) Vesicles. Dekker, New York, pp 373–444
Vinckier A, Semenza G (1998) FEBS Lett 430:12
Niggemann G, Kummrow M, Helfrich W (1995) J Phys II 5:413
Kummrow M, Helfrich W (1991) Phys Rev E 44:8356
Schneider MB, Jenkins JT, Webb WW (1984) J Phys 45:1457
Evans E, Rawicz W (1990) Phys Rev Lett 64:2094
Méléard P, Mitov MD, Faucon JF, Bothorel P (1990) Europhys Lett 11:355
Mutz M, Helfrich W (1990) J Phys 51:991
Dobereiner HG, Evans E, Kraus M, Seifert U, Wortis M (1997) Phys Rev E 55:4458
Farago B, Richter D, Huang JS, Safran SA, Milner ST (1990) Phys Rev Lett 65:3348
Gradzielski M, Langevin D, Farago B (1996) Phys Rev E 53:3900
Farago B (1996) Physica B 226:51
Van Zanten JH (1996) In: Rosoff M (ed) Vesicles. Dekker, New York, pp 239–295
Joannic R, Auvray L, Lasic D (1997) Phys Rev Lett 78:3402
Ferraretto A, Pitto M, Palestini P, Masserini M (1997) Biochemistry 36:9232
Loughrey HC, Ferraretto A, Cannon AM, Acerbis G, Sudati F, Bottiroli G, Masserini M, Soria MR (1993) FEBS Lett 332:183
Hope MJ, Bally MB, Webb G, Cullis PR (1985) Biochim Biophys Acta 812: 55
Tettamanti G, Bonali F, Marchesini S, Zambotti V (1973) Biochim Biophys Acta 296:160
Lewis RN, Mak N, McElhaney RN (1987) Biochemistry 26:6118
Stewart JCM (1980) Anal Biochem 104:10
Corti M (1985) In: Degiorgio V, Corti M (eds) Physics of amphiphiles: micelles, vesicles and microemulsions. North-Holland, Amsterdam, pp 122–151
Milner ST, Safran S (1987) Phys Rev A 36:4371
Cantù L, Corti M, Lago P, Musolino M (1991) Photon correlation spectroscopy: multicomponent systems. SPIE 1430:144
Cantù L, Corti M, Del Favero E, Raudino A (1994) J Phys 4:1585
Cantù L, Corti M, Del Favero E, Dubois M, Zemb T (1998) J Phys Chem B 102:5737
Helfrich W (1973) Z Naturforsch 28:693
Helfrich W (1990) In: Charvolin J, Joanny JF, Zinn-Justin J (eds) Liquids at interfaces. Elsevier Science, Amsterdam, pp 33
Faucon JF, Mitov MD, Méléard P, Bivas I, Bothorel P (1989) J Physique 50:2389
Komura S (1996) In: Rosoff M (ed) Vesicles. Dekker, New York, pp 197–236
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Brocca, P., Cantù, L., Corti, M., Del Favero, E. (2000). Thermal fluctuations of small vesicles: observation by dynamic light scattering. In: Buckin, V. (eds) Trends in Colloid and Interface Science XIV. Progress in Colloid and Polymer Science, vol 115. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-46545-6_37
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DOI: https://doi.org/10.1007/3-540-46545-6_37
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