Abstract
The recombinant proteins produced from Escherichia coli as a host cell need to be made at as low a cost as possible because of the end of the monopoly following expiry of the patent on early pharmaceutical proteins, and thus expanding applications to non-pharmaceutical large-scale products. We review in this article how the various promoters used in recombinant E. coli could affect its protein products, especially with emphasis on relatively new oxygen-dependent nar promoters for β-galactosidase production. Several studies carried out in the authors’ laboratory show that the nar promoter does not require any chemicals except 1 % nitrate and oxygen for protein production. And according to recent work with the modified strains it is possible to produce the enzyme (β-galactosidase) even without the nitrate ions at 45% of its total protein content when its cell density reached OD = 176.
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Dedicated to Prof. Dr. Wolf-Dieter Deckwer on the occasion of his 60th birthday
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Chang, H.N., Han, S.J., Yim, SC., Han, Mr., Lee, J. (2002). Fed-Batch Cultures of Escherichia coli Cells with Oxygen-Dependent nar Promoter Systems. In: Schügerl, K., et al. Tools and Applications of Biochemical Engineering Science. Advances in Biochemical Engineering/Biotechnology, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45736-4_8
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DOI: https://doi.org/10.1007/3-540-45736-4_8
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