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Cell Retention Devices for Suspended-Cell Perfusion Cultures

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Book cover Tools and Applications of Biochemical Engineering Science

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 74))

Abstract

Perfusion cultures of animal cells have several advantages over batch or fed-batch cultures. They give, for instance, higher productivities and a consistent product quality, and allow steady state operation and better cell physiology control. However, one of the main aspects limiting performance and scale-up of perfusion processes is the need for an adequate cell retention device. The devices currently in use for stirred perfusion bioreactors are continuous centrifuges, tangential flow membrane filters, dynamic filters, spin-filters, ultrasonic and di- electrophoretic separators, gravity settlers and, more recently, hydrocyclones. The advantages and disadvantages of each of these methods will be discussed.

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  1. Leda R. Castilho

    Present address: Biochemical Engineering Division, GBF-German National Research Center for Biotechnology, Mascheroder Weg 1, 38124, Braunschweig, Germany

Authors and Affiliations

  1. Department of Chemical Engineering, School of Chemistry, Centro de Tecnologia, Federal University of Rio de Janeiro, Bloco E, Ilha do Fundão, 21949-900, Rio de Janeiro - RJ, Brazil

    Ricardo A. Medronho

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Editors and Affiliations

  1. Institute of Technical Chemistry, University of Hannover, Callinstraße 3, 30167, Hannover, Germany

    K. Schügerl

  2. GBF Biochemical Engineering Division, Mascheroder Weg 1, 38124, Braunschweig, Germany

    A.-P. Zeng

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Dedicated to Prof. Dr. Wolf-Dieter Deckwer on the occasion of his 60th birthday

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Castilho, L.R., Medronho, R.A. (2002). Cell Retention Devices for Suspended-Cell Perfusion Cultures. In: Schügerl, K., et al. Tools and Applications of Biochemical Engineering Science. Advances in Biochemical Engineering/Biotechnology, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45736-4_7

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