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Tools and Applications of Biochemical Engineering Science pp 1–19Cite as

Methods for Biocatalyst Screening

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Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 74))

Abstract

Biocatalysts are now widely accepted as useful alternative tools to classic organic synthetic techniques for the regio- and enantioselective synthesis under mild reaction conditions in many fields of chemistry. The development of techniques for the rational or evolutionary design of novel or modified enzymes has increased the need for fast and reliable methods for the identification of the most powerful catalysts. We present a short overview on screening techniques in this area. Beside classical methods such as spectrophotometry and fluorimetry, a number of new approaches like methods based on the measurement of pH changes or IR-thermography have been recently developed. Additionally the use of electrospray and matrix-assisted laser desorption/ionization mass spectrometry has gained increasing influence in this field of biotechnology.

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Author information

Authors and Affiliations

  1. Technische Biochemie, Universität des Saarlandes, Im Stadtwald, Geb. 2, 66123, Saarbrücken, Germany

    Andreas Tholey & Elmar Heinzle

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  1. Andreas Tholey
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  2. Elmar Heinzle
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Editor information

Editors and Affiliations

  1. Institute of Technical Chemistry, University of Hannover, Callinstraße 3, 30167, Hannover, Germany

    K. Schügerl

  2. GBF Biochemical Engineering Division, Mascheroder Weg 1, 38124, Braunschweig, Germany

    A.-P. Zeng

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Dedicated to Prof. Dr. Wolf-Dieter Deckwer on the occasion of his 60th birthday

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© 2002 Springer-Verlag Berlin Heidelberg

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Tholey, A., Heinzle, E. (2002). Methods for Biocatalyst Screening. In: Schügerl, K., et al. Tools and Applications of Biochemical Engineering Science. Advances in Biochemical Engineering/Biotechnology, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45736-4_1

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  • DOI: https://doi.org/10.1007/3-540-45736-4_1

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-42250-1

  • Online ISBN: 978-3-540-45736-7

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