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Unravelling Carbohydrate Interactions with Biosensors Using Surface Plasmon Resonance (SPR) Detection

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Host-Guest Chemistry

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 218))

Abstract

Surface plasmon resonance (SPR) is an optical phenomenon occurring at a metal coated interface between two media of different refractive index, e.g., water and glass. Exploitation of this phenomenon for investigating biomolecular interactions has occurred since biomolecules can be attached to a chemically modified gold surface and that an interaction between the surface and other biomolecules in solution will affect the SPR of the system. A great deal of the literature on this subject has involved an investigation of protein-ligand interactions, but this chapter will review the use of this technique for investigating carbohydrate-ligand interactions.

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Haseley, S.R., Kamerling, J.P., Vliegenthart, J.F.G. (2002). Unravelling Carbohydrate Interactions with Biosensors Using Surface Plasmon Resonance (SPR) Detection. In: Penadés, S. (eds) Host-Guest Chemistry. Topics in Current Chemistry, vol 218. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45010-6_3

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