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An oxygen-independent ethanol sensor based on quinohemoprotein alcohol dehydrogenase covalently bound to a functionalized polypyrrole film

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Surface and Colloid Science

Part of the book series: Progress in Colloid and Polymer Science ((PROGCOLLOID,volume 116))

Abstract

In the present work the characteristics of a phenazine methosulphate mediated alcohol biosensor based on a newly isolated quinohemoprotein alcohol dehydrogenase are described. The enzyme was covalently linked at a functionalized polypyrrole film which had been electrochemically deposited on the surface of a platinum-black electrode. The biosensor architecture developed was characterized with regard to sensitivity, selectivity, and long-term operational stability. Owing to the inherent properties of the new enzyme the related biosensors are oxygen-independent and exhibit improved selectivity to ethanol in contrast to alcohol biosensors based on alcohol oxidase or on cationic nicotinamide adenine dinucleotide dependent alcohol dehydrogenase.

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Authors and Affiliations

  1. Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 22, 2006, Vilnius, Lithuania

    A. Ramanavicius

  2. Analytische Chemie — Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780, Bochum, Germany

    K. Habermüller & W. Schuhmann

  3. Laboratory of Bioanalysis, Institute of Biochemistry, Mokslininku 12, 2600, Vilnius, Lithuania

    J. Razumiene, R. Meškys, L. Marcinkeviciene, I. Bachmatova & V. Laurinavicius

  4. Department of Biotechnology, University of Lund, P.O. Box 124, 221 00, Lund, Sweden

    E. Csöregi

Authors
  1. A. Ramanavicius
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  2. K. Habermüller
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  3. J. Razumiene
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  4. R. Meškys
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  5. L. Marcinkeviciene
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  7. E. Csöregi
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  8. V. Laurinavicius
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  9. W. Schuhmann
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Editor information

Valdemaras Razumas Björn Lindman Tommy Nylander

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© 2000 Springer-Verlag

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Ramanavicius, A. et al. (2000). An oxygen-independent ethanol sensor based on quinohemoprotein alcohol dehydrogenase covalently bound to a functionalized polypyrrole film. In: Razumas, V., Lindman, B., Nylander, T. (eds) Surface and Colloid Science. Progress in Colloid and Polymer Science, vol 116. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-44941-8_23

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  • DOI: https://doi.org/10.1007/3-540-44941-8_23

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-67814-4

  • Online ISBN: 978-3-540-44941-6

  • eBook Packages: Springer Book Archive

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