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Pattern Discovery in RNA Secondary Structure Using Affix Trees

  • Giancarlo Mauri
  • Giulio Pavesi
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 2676)

Abstract

We present an algorithm for finding common secondary structure motifs in a set of unaligned RNA sequences. The basic version of the algorithm takes as input a set of strings representing the secondary structure of the sequences, enumerates a set of candidate secondary structure patterns, and finally reports all those patterns that appear, possibly with variations, in all or most of the sequences of the set. By considering structural information only, the algorithm can be applied to cases where the input sequences do not present any significant similarity. However, sequence information can be added to the algorithm at different levels. Patterns describing RNA secondary structure elements present a peculiar symmetric layout that makes affix trees a suitable indexing structure that significantly accelerates the searching process, by permitting bidirectional search from the middle to the outside of patterns. In case the secondary structure of the input sequences is not available, we show how the algorithm can deal with the uncertainty deriving from prediction methods, or can predict the structure by itself on the fly while searching for patterns, again taking advantage of the information contained in the affix tree built for the sequences. Finally, we present some case studies where the algorithm was able to detect experimentally known RNA stem-loop motifs, either by using predicted structures, or by folding the sequences by itself.

Keywords

Secondary Structure Input Sequence Loop Structure Lowercase Letter Internal Loop 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Giancarlo Mauri
    • 1
  • Giulio Pavesi
    • 1
  1. 1.Dept. of Computer Science, Systems and CommunicationUniversity of Milan-BicoccaMilanItaly

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