Physicochemical and chemical characterisation of chitosan in dilute aqueous solution

  • Gisela Berth
  • Helmut Cölfen
  • Herbert Dautzenberg
Conference paper
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 119)


Static and dynamic light scattering as well as analytical ultracentrifugation and viscosity measurements were used to investigate the chain conformation of chitosans in salt-containing solutions (pH 4.5; ionic strength about 0.12 M). The samples of various degrees of acetylation were chemically homogeneous. The molecular-weight dependence of the radius of gyration has given clear evidence for a relatively flexible wormlike chain with a persistence length of about 6 nm irrespective of the degree of acetylation, where excluded-volume effects and the polydispersity of the samples were taken into account. In contrast, the interpretation of the hydrodynamic data via a “whole-body approach” according to the Wales-van Holde ratio suggested a strongly elongated chain conformation. The failure of the latter to properly reflect the chain conformation was ascribed to the high extent of draining. A nearly free-draining case can also account for the high scaling exponent of the relationship between intrinsic viscosity and molecular mass.

Key words

Chitosan Wormlike chain Draining effects Light scattering Analytical ultracentifugation 


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  1. 1.
    Berth G, Dautzenberg H (1998) Recent Res Dev Macromol Res 3:225–248Google Scholar
  2. 2.
    Berth G, Dautzenberg H, Peter MG (1998) Carbohydr Polym 36:205–216CrossRefGoogle Scholar
  3. 3.
    Cölfen H, Berth G, Dautzenberg H (2001) Carbohydr Polym 45:373–383CrossRefGoogle Scholar
  4. 4.
    Berth G, Dautzenberg H (2002) Carbohydr Polym 47:39–51CrossRefGoogle Scholar
  5. 5.
    Terbojevich M, Cosani A, Conio G, Marsano E, Bianchi E (1991) Carbohydr Res 209:251–260CrossRefGoogle Scholar
  6. 6.
    Beri RG, Walker J, Reese ET, Rollings JE (1993) Carbohydr Res 238:11–26CrossRefGoogle Scholar
  7. 7.
    Wu C, Zhou SQ, Wang W (1995) Biopolymers 35:385–392CrossRefGoogle Scholar
  8. 8.
    Rinaudo M, Milas M, Dung PL (1993) Int J Biol Macromol 15:281–285CrossRefGoogle Scholar
  9. 9.
    Yamakawa H (1971) Modern theory of polymer solutions. Harper and Row, New YorkGoogle Scholar
  10. 10.
    Ottøy MH, Vårum KM, Christensen BE, Anthonsen MW, Smidsrød O (1996) Carbohydr Polym 31:253–261CrossRefGoogle Scholar
  11. 11.
    Errington N, Harding SE, Vårum KM, Illum L (1993) Int J Biol Macromol 15: 113–117CrossRefGoogle Scholar
  12. 12.
    Lavrenko PN, Linow KJ, Görnitz E (1992) In: Harding SE, Rowe AJ, Horton JC (eds) Analytical ultracen-trifugation in biochemistry and polymer science. The Royal Society of Chemistry, Cambridge, pp 517–531Google Scholar
  13. 13.
    Wales M, van Holde KE (1954) J Polym Sci 14:81–86CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Gisela Berth
    • 1
  • Helmut Cölfen
    • 1
  • Herbert Dautzenberg
    • 1
  1. 1.Max Planck Institute for Colloids and Interface ResearchPotsdamGermany

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