Study of polyelectrolyte complex formation applying the synthetic boundary technique of analytical ultracentrifugation

  • Christine Wandrey
  • Gabriela Grigorescu
  • David Hunkeler
Conference paper
Part of the Progress in Colloid and Polymer Science book series (PROGCOLLOID, volume 119)


Polyelectrolyte complex membrane formation has been studied, on-line, using the synthetic boundary technique of an Optima XL-I analytical ultracentrifuge. This recent method, established by the first author, has now been evaluated concerning its sensitivity to monitor differences in the membrane formation if the membrane components and experimental conditions are varied. For this purpose the membrane formation of sodium alginate with three polycations, poly(vinyl-amine) hydrochloride, poly(L-ly-sine) hydrochloride, and chitosan oligomers, was examined under various experimental conditions. In addition, two approaches have been developed and applied to evaluate the membrane formation process and the membrane quality on the basis of the experimentally received radial absorption scans. The first approach generalizes the membrane growth and its direction, resulting in a time-dependent description of the membrane geometry. The second one considers the membrane thickness as a function of time and might, therefore, serve to calculate the growth velocity or shrinking of the membrane, but not the directions of growth or shrinking. The investigations revealed a sufficient sensitivity of the method leading to the conclusion that the membranes formed in this study differ in thickness, symmetry, homogeneity, and the kinetics of formation. Consequently, the demonstrated sensitivity of this ultracentrifugation technique offers novel extended possibilities for basic studies of the polyelectrolyte complex membrane formation. Moreover, the goal-directed optimization of such membranes for practical applications can be supported.

Key words

Analytical ultracentrifugation Polyelectrolyte complexes Synthetic boundary Membrane formation 


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  1. 1.
    Dautzenberg H, Jaeger W, Kötz J, Philipp B, Seidel C, Stscherbina D (1994) Polyelectrolytes: formation, characterization and application. Hanser, MunichGoogle Scholar
  2. 2.
    Hunkeler D (1997) Trends Polym Sci 5:286Google Scholar
  3. 3.
    Gander B, Blanco-Prieto MJ, Thomasin C, Wandrey C, Hunkeler D (2001) In:Swarbrick J, Boylan JC (eds) Encyclopedia of pharmaceutical technology, 2nd edn. Dekker, New York, pp 481Google Scholar
  4. 4.
    Prokop A, Hunkeler D, Powers AC, Whitesell RR, Wang TG (1998) Adv Polym Sci136:53CrossRefGoogle Scholar
  5. 5.
    Angelova N, Hunkeler D (1999) Trends Biotechnol 17:409CrossRefGoogle Scholar
  6. 6.
    Philipp B, Dautzenberg H, Linow KJ, Kötz J, Dawydoff W (1989) Prog Polym Sci 14:91CrossRefGoogle Scholar
  7. 7.
    Arabi H, Hashemi SA, Fooladi M (1996) J Microencapsul 13:527CrossRefGoogle Scholar
  8. 8.
    Lukas J, Richau K, Schwarz HH, Paul D (1997) J Membr Sci 131:39CrossRefGoogle Scholar
  9. 9.
    Karibyants N, Dautzenberg H, Cölfen H(1997) Macromolecules 30:7803CrossRefGoogle Scholar
  10. 10.
    Prokop A, Hunkeler D, Haralson M, Dimari S, Wang TG(1998) Adv Polym Sci 136:1CrossRefGoogle Scholar
  11. 11.
    Wandrey C, Bartkowiak A, Hunkeler D(2000) In: Transactions of the 6th world biomaterials congress, May 15-20, 2000, Hawaii, USA, vol II. Society for Biomaterials, USA, p 893Google Scholar
  12. 12.
    Wandrey C (2000) Polym News 25:299Google Scholar
  13. 13.
    Wandrey C, Bartkowiak A (2001) Colloids Surf A 180:141CrossRefGoogle Scholar
  14. 14.
    Van Treslong Bloys CJ, Morra CFH (1975) J R Neth Chem Soc 94:101Google Scholar
  15. 15.
    Mullagaliev IR (1995) Dokl Akad Nauk 345:199Google Scholar
  16. 16.
    Bartkowiak A, Hunkeler D (2000) Chem Mater 12:206CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Christine Wandrey
    • 1
  • Gabriela Grigorescu
    • 1
  • David Hunkeler
    • 1
  1. 1.Laboratory of Polyelectrolytes and Biomacromolecules Department of ChemistrySwiss Federal Institute of TechnologyLausanneSwitzerland

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