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Polypeptide N-acetylgalactosaminyltransferase

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Class 2 · Transferases IV

Part of the book series: Springer Handbook of Enzymes ((HDBKENZYMES,volume 31))

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References

  1. Sugiura, M.; Kawasaki, T.; Yamashina, I.: Purification and characterization of UDP-GalNAc:polypeptide N-acetylgalactosamine transferase from an ascites hepatoma, AH 66. J. Biol. Chem., 257, 9501–9507 (1982)

    PubMed  CAS  Google Scholar 

  2. Takeuchi, M.; Yoshikawa, M.; Sasaki, R.; Chiba, R.: Purification and characterization of UDP-N-acetylgalactosamine:κ-casein polypeptide N-acetylgalactosaminyltransferase from mammary gland of lacting cow. Agric. Biol. Chem., 49, 1059–1069 (1985)

    CAS  Google Scholar 

  3. Wang, Y.; Agrawal, N.; Eckhardt, A.E.; Stevens, R.D.; Hill, R.L.: The acceptor substrate specificity of porcine submaxillary UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is dependent on the amino acid sequences adjacent to serine and threonine residues. J. Biol. Chem., 268, 22979–22983 (1993)

    PubMed  CAS  Google Scholar 

  4. Wang, Y.; Abernethy, J.L.; Eckhardt, A.E.; Hill, R.L.: Purification and characterization of a UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase specific for glycosylation of threonine residues. J. Biol. Chem., 267, 12709–12716 (1992)

    PubMed  CAS  Google Scholar 

  5. Hatanaka, K.; Slama, J.T.; Elbein, A.D.: Synthesis of a new inhibitor of the UDP-GalNAc:polypeptide galactosaminyl transferase. Biochem. Biophys. Res. Commun., 175, 668–672 (1991)

    Article  PubMed  CAS  Google Scholar 

  6. Elhammer, A.; Kornfeld, S.: Purification and characterization of UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyltransferase from bovine colostrum and murine lymphoma BW5147 cells. J. Biol. Chem., 261, 5249–5255 (1986)

    PubMed  CAS  Google Scholar 

  7. Briand, J.P.; Andrews, S.P.; Cahill, E.; Conway, N.A.; Young, J.D.: Investigation of the requirements for O-glycosylation by bovine submaxillary gland UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosamine transferase using synthetic peptide substrates. J. Biol. Chem., 256, 12205–12207 (1981)

    PubMed  CAS  Google Scholar 

  8. Cottrell, J.M.; Hall, R.L.; Sturton, R.G.; Kent, P.W.: Polypeptide N-acetylgalactosaminyltransferase activity in tracheal epithelial microsomes. Biochem. J., 283, 299–305 (1992)

    PubMed  CAS  Google Scholar 

  9. Homa, F.L.; Baker, C.A.; Thomsen, D.R.; Elhammer, A.P.: Conversion of a bovine UDP-GalNAc:polypeptide, N-acetylgalactosaminyltransferase to a soluble, secreted enzyme, and expression in Sf9 cells. Protein Expr. Purif., 6, 141–148 (1995)

    Article  PubMed  CAS  Google Scholar 

  10. Brockhausen, I.; Toki, D.; Brockhausen, J.; Peters, S.; Bielfeldt, T.; Kleen, A.; Paulsen, H.; Meldal, M.; Hagen, E; Tabak, L.A.: Specificity of O-glycosylation by bovine colostrum UDP-GalNAc: polypeptide α-N-acetylgalactosaminyltransferase using synthetic glycopeptide substrates. Glycoconjugate J., 13, 849–856 (1996)

    Article  CAS  Google Scholar 

  11. Mendicino, J.; Sangadala, S.: Purification and characterization of UDP-Gal-NAc:polypeptide N-acetylgalactosaminyltransferase from swine trachea epithelium. Mol. Cell. Biochem., 185, 135–145 (1998)

    Article  PubMed  CAS  Google Scholar 

  12. Soerensen, T.; White, T.; Wandall, H.H.; Kristensen, A.K.; Roepstorff, P.; Clausen, H.: UDP-N-acetyl-α-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase. Identification and separation of two distinct transferase activities. J. Biol. Chem., 270, 24166–24173 (1995)

    Article  CAS  Google Scholar 

  13. Wandall, H.H.; Hassan, H.; Mirgorodskaya, E.; Kristensen, A.K.; Roepstorff, P.; Bennett, E.P.; Nielsen, P.A.; Hollingsworth, M.A.; Burchell, J.; Taylor-Pa-padimitriou, J.; Clausen, H.: Substrate specificities of three members of the human UDP-N-acetyl-α-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase family, GalNAc-T1,-T2, and-T3. J. Biol. Chem., 272, 23503–23514 (1997)

    Article  PubMed  CAS  Google Scholar 

  14. Bennett, E.P.; Hassan, H.; Hollingsworth, M.A.; Clausen, H.: A novel human UDP-N-acetyl-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates. FEBS Lett., 460, 226–230 (1999)

    Article  PubMed  CAS  Google Scholar 

  15. Toba, S.; Tenno, M.; Konishi, M.; Mikami, T.; Itoh, N.; Kurosaka, A.: Brain-specific expression of a novel human UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T9). Biochim. Biophys. Acta, 1493, 264–268 (2000)

    PubMed  CAS  Google Scholar 

  16. Ten Hagen, K.G.; Bedi, G.S.; Tetaert, D.; Kingsley, P.D.; Hagen, F.K.; Balys, M.M.; Beres, T.M.; Degand, P.; Tabak, L.A.: Cloning and characterization of a ninth member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family, ppGaNTase-T9. J. Biol. Chem., 276, 17395–17404 (2001)

    Article  PubMed  Google Scholar 

  17. Wang, H.; Tachibana, K.; Zhang, Y.; Iwasaki, H.; Kameyama, A.; Cheng, L.; Guo, J.; Hiruma, T.; Togayachi, A.; Kudo, T.; Kikuchi, N.; Narimatsu, H.: Cloning and characterization of a novel UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, pp-GalNAc-T14. Biochem. Biophys. Res. Commun., 300, 738–744 (2003)

    Article  PubMed  CAS  Google Scholar 

  18. Zhang, Y.; Iwasaki, H.; Wang, H.; Kudo, T.; Kalka, T.B.; Hennet, T.; Kubota, T.; Cheng, L.; Inaba, N.; Gotoh, M.; Togayachi, A.; Guo, J.; Hisatomi, H.; Nakajima, K.; Nishihara, S.; Nakamura, M.; Marth, J.D.; Narimatsu, H.: Cloning and characterization of a new human UDP-N-acetyl-α-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase, designated pp-Gal-NAc-T13, that is specifically expressed in neurons and synthesizes GalNAc a-serine/threonine antigen. J. Biol. Chem., 278, 573–584 (2003)

    Article  PubMed  CAS  Google Scholar 

  19. Ten Hagen, K.G.; Tetaert, D.; Hagen, F.K.; Richet, C.; Beres, T.M.; Gagnon, J.; Balys, M.M.; VanWuyckhuyse, B.; Bedi, G.S.; Degand, P.; Tabak, L.A.: Characterization of a UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase that displays glycopeptide N-acetylgalactosaminyltransferase activity. J. Biol. Chem., 274, 27867–27874 (1999)

    Article  PubMed  Google Scholar 

  20. Elhammer, A.P.; Poorman, R.A.; Brown, E.; Maggiora, L.L.; Hoogerheide, J.G.; Kezdy, F.J.: The specificity of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase as inferred from a database of in vivo substrates and from the in vitro glycosylation of proteins and peptides. J. Biol. Chem., 268, 10029–10038 (1993)

    PubMed  CAS  Google Scholar 

  21. Hanisch, F.G.; Reis, C.A.; Clausen, H.; Paulsen, H.: Evidence for glycosylation-dependent activities of polypeptide N-acetylgalactosaminyltransferases rGalNAc-T2 and-T4 on mucin glycopeptides. Glycobiology, 11, 731–740 (2001)

    Article  PubMed  CAS  Google Scholar 

  22. Iwasaki, H.; Zhang, Y.; Tachibana, K.; Gotoh, M.; Kikuchi, N.; Kwon, Y.D.; Togayachi, A.; Kudo, T.; Kubota, T.; Narimatsu, H.: Initiation of O-glycan synthesis in IgA1 hinge region is determined by a single enzyme, UDP-N-acetyl-α-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2. J. Biol. Chem., 278, 5613–5621 (2003)

    Article  PubMed  CAS  Google Scholar 

  23. Hagen, F.K.; Hazes, B.; Raffo, R.; deSa, D.; Tabak, L.A.: Structure-function analysis of the UDP-N-acetyl-d-galactosamine: polypeptide N-acetylgalactosaminyltransferase. Essential residues lie in a predicted active site cleft resembling a lactose repressor fold. J. Biol. Chem., 274, 6797–6803 (1999)

    Article  PubMed  CAS  Google Scholar 

  24. Tenno, M.; Saeki, A.; Kezdy, F.J.; Elhammer, A.P.; Kurosaka, A.: The lectin domain of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 is involved in O-glycosylation of a polypeptide with multiple acceptor sites. J. Biol. Chem., 277, 47088–47096 (2002)

    Article  PubMed  CAS  Google Scholar 

  25. Ten Hagen, K.G.; Tran, D.T.: A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is essential for viability in Drosophila melanogaster. J. Biol. Chem., 277, 22616–22622 (2002)

    Article  PubMed  Google Scholar 

  26. Hennebicq, S.; Tetaert, D.; Soudan, B.; Briand, G.; Richet, C.; Demeyer, D.; Gagnon, J.; Petillot, Y.; Degand, P.: Polypeptide:N-acetylgalactosaminyltransferase activities towards the mucin MUC5AC peptide motif using microsomal preparations of normal and tumoral digestive mucosa. Biochimie, 80, 69–73 (1998)

    Article  PubMed  CAS  Google Scholar 

  27. Takeuchi, H.; Kato, K.; Hassan, H.; Clausen, H.; Irimura, T.: O-GalNAc incorporation into a cluster acceptor site of three consecutive threonines. Distinct specificity of GalNAc-transferase isoforms. Eur. J. Biochem., 269, 6173–6183 (2002)

    Article  PubMed  CAS  Google Scholar 

  28. Wojczyk, B.S.; Stwora-Wojczyk, M.M.; Hagen, F.K.; Striepen, B.; Hang, H.C.; Bertozzi, C.R.; Roos, D.S.; Spitalnik, S.L.: cDNA cloning and expression of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase T1 from Toxoplasma gondii. Mol. Biochem. Parasitol., 131, 93–107 (2003)

    Article  PubMed  CAS  Google Scholar 

  29. Katao, K.; Takeuchi, H.; Miyahara, N.; Kanoh, A.; Hassan, H.; Clausen, H.; Irimura, T.: Distinct orders of GalNAc incorporation into a peptide with consecutive threonines. Biochem. Biophys. Res. Commun., 287, 110–115 (2001)

    Article  Google Scholar 

  30. Iida, S.I.; Takeuchi, H.; Kato, K.; Yamamoto, K.; Irimura, T.: Order and maximum incorporation of N-acetyl-d-galactosamine into threonine residues of MUC2 core peptide with microsome fraction of human-colon-carcinoma LS174T cells. Biochem. J., 347, 535–542 (2000)

    Article  PubMed  CAS  Google Scholar 

  31. Hagen, F.K.; Nehrke, K.: cDNA cloning and expression of a family of UDP-N-acety1-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans. J. Biol. Chem., 273, 8268–8277 (1998)

    Article  PubMed  CAS  Google Scholar 

  32. Wragg, S.; Hagen, F.K.; Tabak, L.A.: Identification of essential histidine residues in UDP-N-acetyl-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T1. Biochem. J., 328, 193–197 (1997)

    PubMed  CAS  Google Scholar 

  33. Hagen, F.K.; Ten Hagen, K.G.; Beres, T.M.; Balys, M.M.; Van Wuyckhuyse, B.C.; Tabak, L.A.: cDNA cloning and expression of a novel UDP-N-acetyl-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase. J. Biol. Chem., 272, 13843–13848 (1997)

    Article  PubMed  CAS  Google Scholar 

  34. Wragg, S.; Hagen, F.K.; Tabak, L.A.: Kinetic analysis of a recombinant UDP-N-acety1-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase. J. Biol. Chem., 270, 16947–16954 (1995)

    Article  PubMed  CAS  Google Scholar 

  35. Roth, J.; Wang, Y.; Eckhardt, A.E.; Hill, R.L.: Subcellular localization of the UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-mediated O-glycosylation reaction in the submaxillary gland. Proc. Natl. Acad. Sci. USA, 91, 8935–8939 (1994)

    Article  PubMed  CAS  Google Scholar 

  36. Nishimaori, I.; Fontenot, J.D.; Hollingsworth, M.A.: N-Acetylgalactosamine glycosylation of MUC1 tandem repeat peptides by pancreatic tumor cell extracts. Cancer Res., 54, 3738–3744 (1994)

    Google Scholar 

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  1. Institute for Biochemistry, University to Cologne, Zülpicher Strasse 47, 50674, Cologne, Germany

    Dietmar Schomburg , Ida Schomburg  & Antje Chang ,  & 

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(2006). Polypeptide N-acetylgalactosaminyltransferase. In: Schomburg, D., Schomburg, I., Chang, A. (eds) Class 2 · Transferases IV. Springer Handbook of Enzymes, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-37718-2_41

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