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Glycylpeptide N-tetradecanoyltransferase

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Springer Handbook of Enzymes

Part of the book series: Springer Handbook of Enzymes ((HDBKENZYMES,volume 30))

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Nomenclature

EC number

2.3.1.97

Systematic name

tetradecanoyl-CoA:glycylpeptide N-tetradecanoyltransferase

Recommended name

glycylpeptide N-tetradecanoyltransferase

Synonyms

NMT <1-12> [1-6, 8-16, 18-21, 23, 24, 26, 28, 30-37, 39, 41-44]

Nmtlp <1> [7, 27, 29, 38, 40, 45]

myristoyl-CoA-protein N-myristoyltransferase

myristoyl-coenzyme A:protein N-myristoyl transferase

myristoylating enzymes

myristoyltransferase, protein N-

peptide N-myristoyltransferase

protein N-myristoyltransferase

Additional information <1> (<1> enzyme belongs to the superfamily of GCN5-related N-acetyltransferases [38,40]) [38, 40]

CAS registry number

110071-61-9

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References

  1. Towler, D.A.; Gordon, J.I.; Adams, S.P.; Glaser, L.: The biology and enzymology of eukaryotic protein acylation. Annu. Rev. Biochem., 57, 69–99 (1988)

    Article  PubMed  CAS  Google Scholar 

  2. Towler, D.E.; Adams, S.R; Eubanks, S.R.; Towery, D.S.; Jackson-Machelski, E.; Glaser, L.; Gordon, J.I.: Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase [published erratum appears in Proc Natl Acad Sci U S A 1987 Nov;84(21):7523]. Proc. Natl. Acad. Sci. USA, 84, 2708–2712 (1987)

    Article  PubMed  CAS  Google Scholar 

  3. Heuckeroth, R.O.; Towler, D.A.; Adams, S.R; Glaser, L.; Gordon, J.I.: 11-(Ethylthio)undecanoic acid. A myristic acid analogue of altered hydrophobicity which is functional for peptide N-myristoylation with wheat germ and yeast acyltransferase. J. Biol. Chem., 263, 2127–2133 (1988)

    PubMed  CAS  Google Scholar 

  4. King, M.J.; Sharma, R.K.: Identification, purification and characterization of a membrane-associated N-myristoyltransferase inhibitor protein from bovine brain. Biochem. J., 291, 635–639 (1993)

    PubMed  CAS  Google Scholar 

  5. Mcllhinney, R.A.J.; McGlone, K.; Willis, A.C.: Purification and partial sequencing of myristoyl-CoA:protein N-myristoyltransferase from bovine brain. Biochem. J., 290, 405–410 (1993)

    Google Scholar 

  6. Wiegand, R.C.; Carr, C; Minnerly, J.C.; Pauley, A.M.; Carron, C.P.; Langner, C.A.; Duronio, R.J.; Gordon, J.I.: The Candida albicans myristoyl-CoA:protein N-myristoyltransferase gene. Isolation and expression in Saccharomyces cerevisiae and Escherichia coli. J. Biol. Chem., 267, 8591–8598 (1992)

    PubMed  CAS  Google Scholar 

  7. Rudnick, D.A.; Rocque, W.J.; McWherter, C.A.; Toth, M.V.; Jackson-Machelski, E.; Gordon, J.I.: Use of photoactivatable peptide substrates of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (Nmtlp) to characterize a myristoyl-CoA-Nmtlp-peptide ternary complex and to provide evidence for an ordered reaction mechanism. Proc. Natl. Acad. Sci. USA, 90,1087–1091(1993)

    Article  PubMed  CAS  Google Scholar 

  8. McIlhenney, R.A.J.; McGlone, K.: Characterization of the myristoyl CoA:-glycylpeptide N-myristoyl transferase from rat and bovine brain. Biochem. Soc. Trans., 20, 341S (1992)

    Google Scholar 

  9. Wagner, A.P.; Retey, J.: Synthesis of myristoyl-carba(dethia)-coenzyme A and S-(3-oxohexadecyl)-coenzyme A, two potent inhibitors of myristoyl-CoArprotein N-myristoyltransferase. Eur. J. Biochem., 195, 699–705 (1991)

    Article  PubMed  CAS  Google Scholar 

  10. Boutin, J.A.; Clarenc, J.P.; Ferry, G.; Ernould, A.P.; Remond, G.; Vincent, M.; Atassi, G.: N-myristoyl-transferase activity in cancer cells. Solubilization, specificity and enzymatic inhibition of a N-myristoyl transferase from L1210 microsomes. Eur. J. Biochem., 201, 257–263 (1991)

    Article  PubMed  CAS  Google Scholar 

  11. Kishore, N.S.; Lu, T.; Knoll, L.J.; Katoh, A.; Rudnick, D.A.; Mehta, P.P.; Devadas, B.; Huhn, M.; Atwood, J.L.; Adams, S.P.; Gokel, G.W.; Gordon, J.I.: The substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase. Analysis of myristic acid analogs containing oxygen, sulfur, double bonds, triple bonds, and/or an aromatic residue. J. Biol. Chem., 266, 8835–8855 (1991)

    PubMed  CAS  Google Scholar 

  12. Rudnick, D.A.; McWherter, C.A.; Rocaque, W.J.; Lennon, P.J.; Getman, D.P.; Gordon, J.I.: Kinetic and structural evidence for a sequential ordered Bi Bi mechanism of catalysis by Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase. J. Biol. Chem., 266, 9732–9739 (1991)

    PubMed  CAS  Google Scholar 

  13. Duronio, R.J.; Rudnick, D.A.; Adams, S.P.; Towler, D.A.; Gordon, J.I.: Analyzing the substrate specificity of Saccharomyces cerevisiae myristoyl-CoA: protein N-myristoyltransferase by co-expressing it with mammalian G protein α subunits in Escherichia coli. J. Biol. Chem., 266, 10498–10504 (1991)

    PubMed  CAS  Google Scholar 

  14. Gordon, J.I.; Duronio, R.J.; Rudnick, D.A.; Adams, S.P.; Gokel, G.W.: Protein N-myristoylation. J. Biol. Chem., 266, 8647–8650 (1991)

    PubMed  CAS  Google Scholar 

  15. Paige, L.A.; Zheng, G.Q.; DeFrees, S.A.; Cassady, J.M.; Geahlen, R.L.: Metabolic activation of 2-substituted derivatives of myristic acid to form potent inhibitors of myristoyl CoA:protein N-myristoyltransferase. Biochemistry, 29, 10566–10573 (1990)

    Article  PubMed  CAS  Google Scholar 

  16. Rudnick, D.A.; McWherter, C.A.; Adams, S.A.; Ropson, I.J.; Duronio, R.J.; Gordon, J.I.: Structural and functional studies of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase produced in Escherichia coli. Evidence for an acyl-enzyme intermediate. J. Biol. Chem., 265, 13370–13378 (1990)

    PubMed  CAS  Google Scholar 

  17. McIlhinney, R.A.J.; McGlone, K.: Characterisation of a myristoyl CoA:glycylpeptide N-myristoyl transferase activity in rat brain: subcellular and regional distribution. J. Neurochem., 54, 110–117 (1990)

    Article  PubMed  CAS  Google Scholar 

  18. Heuckeroth, R.O.; Jackson-Machelski, E.; Adams, S.P.; Kishore, N.S.; Huhn, M.; Katoh, A.; Lu, T.; Gokel, G.W.; Gordon, J.I.: Novel fatty acyl substrates for myristoyl-CoA:protein N-myristoyl-transferase. J. Lipid Res., 31, 1121–1129 (1990)

    PubMed  CAS  Google Scholar 

  19. Paige, L.A.; Chafin, D.R.; Cassady, J.M.; Geahlen, R.L.: Detection of myristoyl CoA:protein N-myristoyltransferase activity by ion-exchange chromatography. Anal. Biochem., 181, 254–258 (1989)

    Article  PubMed  CAS  Google Scholar 

  20. McIlhinney, R.A.J.; McGlone, K.: Characterization, regional and subcellular distribution of an N-myristoyl-CoA:glycyltransferase activity in rat brain. Biochem. Soc. Trans., 17, 888–889 (1989)

    CAS  Google Scholar 

  21. Heuckeroth, R.O.; Glaser, L.; Gordon, J.I.: Heteroatom-substituted fatty acid analogs as substrates for N-myristoyltransferase: an approach for studying both the enzymology and function of protein acylation. Proc. Natl. Acad. Sci. USA, 85, 8795–8799 (1988)

    Article  PubMed  CAS  Google Scholar 

  22. Glover, C.J.; Goddard, C; Felsted, R.L.: N-myristoylation of p60src. Identification of a myristoyl-CoA:glycylpeptide N-myristoyltransferase in rat tissues. Biochem. J., 250, 485–491 (1988)

    PubMed  CAS  Google Scholar 

  23. Towler, D.A.; Eubanks, S.R.; Towery, D.S.; Adams, S.P.; Glaser, L.: Aminoterminal processing of proteins by N-myristoylation. Substrate specificity of N-myristoyl transferase. J. Biol. Chem., 262, 1030–1036 (1987)

    PubMed  CAS  Google Scholar 

  24. Towler, D.A.; Adams, S.P.; Eubanks, S.R.; Towery, D.S.; Jackson-Machelski, E.; Glaser, L.; Gordon, J.I.: Myristoyl CoA: protein N-myristoyltransferase activities from rat liver and yeast possess overlapping yet distinct peptide substrate specificities. J. Biol. Chem., 263, 1784–1790 (1988)

    PubMed  CAS  Google Scholar 

  25. Duronio, R.J.; Read, S.I.; Gordon, J.I.: Mutations of human myristoyl-CoA: protein N-myristoyltransferase cause temperature-sensitive myristic acid auxotrophy in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA, 89, 4129–4133 (1992)

    Article  PubMed  CAS  Google Scholar 

  26. King, M.J.; Sharma, R.K.: Differential activation of bovine brain N-myristoyltransferase(s) by a cytosolic activator. Biochem. Biophys. Res. Commun., 212, 580–588 (1995)

    Article  PubMed  CAS  Google Scholar 

  27. Lu, T.; Li, Q.; Katoh, A.; Hernandez, J.; Duffin, K.; Jackson-Machelski, E.; Knoll, L.J.; Gokel, G.W.; Gordon, J.I.: The substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase. Polar probes of the enzyme’s myristoyl-CoA recognition site. J. Biol. Chem., 269, 5346–5357 (1994)

    PubMed  CAS  Google Scholar 

  28. Glover, C.J.; Felsted, R.L.: Identification and characterization of multiple forms of bovine brain N-myristoyltransferase. J. Biol. Chem., 270, 23226–23233 (1995)

    Article  PubMed  CAS  Google Scholar 

  29. Zhang, L.; Jackson-Machelski, E.; Gordon, J.I.: Biochemical studies of Saccharomyces cerevisiae myristoyl-coenzyme A:protein N-myristoyltransferase mutants. J. Biol. Chem., 271, 33131–33140 (1996)

    Article  PubMed  CAS  Google Scholar 

  30. Raju, R.V.S.; Datla, R.S.S.; Sharma, R.K.: Expression of human N-myristoyltransferase in Escherichia coli. Comparison with N-myristoyltransferases expressed in different tissues. Mol. Cell. Biochem., 155, 69–76 (1996)

    Article  PubMed  CAS  Google Scholar 

  31. Raju, R.V.S.; Datla, R.S.S.; Kakkarl, R.; Sharma, R.K.: Recombinant bovine spleen myristoyl CoA:protein N-myristoyltransferase. Mol. Cell. Biochem., 189, 91–97 (1998)

    Article  PubMed  CAS  Google Scholar 

  32. McIlhinney, R.A.J.; McGlone, K.: Immunocytochemical characterization and subcellular localization of human myristoyl-CoA:protein N-myristoyltransferase in HeLa cells. Exp. Cell Res., 223, 348–356 (1996)

    Article  PubMed  CAS  Google Scholar 

  33. Young, K.; Egerton, M.; Camble, R.; White, A.; McIlhinney, R.A.J.: Immuno-chemical characterization of human N-myristoyltransferase: evidence for more than one form of the enzyme. Biochem. Soc. Trans., 25, S631 (1997)

    PubMed  CAS  Google Scholar 

  34. Raju, R.V.S.; Kakkar, R.; Datla, R.S.S.; Radhi, J.; Sharma, R.K.: Myristoyl-CoA:protein N-myristoyltransferase from bovine cardiac muscle: molecular cloning, kinetic analysis, and in vitro proteolytic cleavage by m-calpain. Exp. Cell Res., 241, 23–35 (1998)

    Article  PubMed  CAS  Google Scholar 

  35. Giang, D.K.; Cravatt, B.F.: A second mammalian N-myristoyltransferase. J. Biol. Chem., 273, 6595–6598 (1998)

    Article  PubMed  CAS  Google Scholar 

  36. Raju, R.V.S.; Datla, R.S.S.; Warrington, R.C.; Sharma, R.K.: Effects of L-histidine and its structural analogs on human N-myristoyltransferase activity and importance of EEVEH amino acid sequence for enzyme activity. Biochemistry, 37, 14928–14936 (1998)

    Article  PubMed  CAS  Google Scholar 

  37. McIlhinney, R.A.; Young, K.; Egerton, M.; Camble, R.; White, A.; Soloviev, M.: Characterization of human and rat brain myristoyl-CoA:protein N-myristoyltransferase: evidence for an alternative splice variant of the enzyme. Biochem. J., 333, 491–495 (1998)

    PubMed  CAS  Google Scholar 

  38. Farazi, T.A.; Manchester, J.K.; Gordon, J.I.: Transient-state kinetic analysis of Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase reveals that a step after chemical transformation is rate limiting. Biochemistry, 39, 15807–15816 (2000)

    Article  PubMed  CAS  Google Scholar 

  39. Qi, Q.; Rajala, R.V.S.; Anderson, W.; Jiang, C; Rozwadowski, K.; Selvaraj, G.; Sharma, R.; Datla, R.: Molecular cloning, genomic organization, and biochemical characterization of myristoyl-CoA:protein N-myristoyltransferase from Arabidopsis thaliana. J. Biol. Chem., 275, 9673–9683 (2000)

    Article  PubMed  CAS  Google Scholar 

  40. Farazi, T.A.; Manchester, J.K.; Waksman, G.; Gordon, J.I.: Pre-steady-state kinetic studies of Saccharomyces cerevisiae myristoylCoA:protein N-myristoyltransferase mutants identify residues involved in catalysis. Biochemistry, 40, 9177–9186 (2001)

    Article  PubMed  CAS  Google Scholar 

  41. Rundle, D.R.; Rajala, R.V.; Anderson, R.E.: Characterization of Type I and Type II myristoyl-CoA:protein N-myristoyltransferases with the Acyl-CoAs found on heterogeneously acylated retinal proteins. Exp. Eye Res., 75, 87–97 (2002)

    Article  PubMed  CAS  Google Scholar 

  42. Takamune, N.; Hamada, H.; Sugawara, H.; Misumi, S.; Shoji, S.: Development of an enzyme-linked immunosorbent assay for measurement of activity of myristoyl-coenzyme A:protein N-myristoyltransferase. Anal. Biochem., 309, 137–142 (2002)

    Article  PubMed  CAS  Google Scholar 

  43. Sogabe, S.; Masubuchi, M.; Sakata, K.; Fukami, T.A.; Morikami, K.; Shiratori, Y.; Ebiike, H.; Kawasaki, K.; Aoki, Y.; Shimma, N.; D’Arcy, A.; Winkler, F.K.; Banner, D.W.; Ohtsuka, T.: Crystal structures of Candida albicans N-myristoyltransferase with two distinct inhibitors. Chem. Biol., 9, 1119–1128 (2002)

    Article  PubMed  CAS  Google Scholar 

  44. Pasha, M.K.; Dimmock, J.R.; Hollenberg, M.D.; Sharma, R.K.: Enhanced activity of human N-myristoyltransferase by dimethyl sulfoxide and related solvents in the presence of serine/threonine-containing peptide substrates. Biochem. Pharmacol., 64, 1461–1467 (2002)

    CAS  Google Scholar 

  45. Farazi, T.A.; Waksman, G.; Gordon, J.I.: Structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoylCoA and peptide provide insights about substrate recognition and catalysis. Biochemistry, 40, 6335–6343 (2001)

    Article  PubMed  CAS  Google Scholar 

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(2006). Glycylpeptide N-tetradecanoyltransferase. In: Schomburg, D., Schomburg, I., Chang, A. (eds) Springer Handbook of Enzymes. Springer Handbook of Enzymes, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-37717-4_38

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