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Deoxycytidylate 5-hydroxymethyltransferase

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Book cover Springer Handbook of Enzymes

Part of the book series: Springer Handbook of Enzymes ((HDBKENZYMES,volume 29))

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Nomenclature

EC number

2.1.2.8

Systematic name

5,10-methylenetetrahydrofolate:deoxycytidylate 5-hydroxymethyltransferase

Recommended name

deoxycytidylate 5-hydroxymethyltransferase

Synonyms

CH

d-cytidine 5′-monophosphate hydroxymethylase

dCMP Hmase

dCMP hydroxymethylase

deoxyCMP hydroxymethylase

deoxycytidylate hydroxymethylase

deoxycytidylate hydroxymethyltransferase

deoxycytidylic hydroxymethylase

hydroxymethyltransferase, deoxycytidylate

CAS registry number

9012-68-4

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References

  1. Mathews, C.K.; Brown, R; Cohen, S.S.: Virus-induced acquisition of metabolic function. VII. Biosynthesis de novo of deoxycytidylate hydroxymethylase. J. Biol. Chem., 239, 2957–2963 (1964)

    PubMed  CAS  Google Scholar 

  2. Pizer, L.I.; Cohen, S.S.: Virus-induced acquisition of metabolic function. V. Purification and properties of deoxycytidylate hydroxymethylase and studies on its origin. J. Biol. Chem., 237, 1251–1259 (1962)

    PubMed  CAS  Google Scholar 

  3. Lamm, N.; Tomaschewski, J.; Ruger, W.: Nucleotide sequence of the deoxycytidylate hydroxymethylase gene of bacteriophage T4 (g42) and the homology of its gene product with thymidylate synthase of E. coli. Nucleic Acids Res., 15, 3920 (1987)

    Article  PubMed  CAS  Google Scholar 

  4. Lamm, N.; Wang, Y.; Mathews, C.K.; Ruger, W.: Deoxycytidylate hydroxymethylase gene of bacteriophage T4. Nucleotide sequence determination and over-expression of the gene. Eur. J. Biochem., 172, 553–563 (1988)

    Article  PubMed  CAS  Google Scholar 

  5. Allen, J.R.; Lasser, G.W.; Goldman, D.A.; Booth, J.W.; Mathews, C.K.: T4 phage deoxyribonucleotide-synthesizing enzyme complex. Further studies on enzyme composition and regulation. J. Biol. Chem., 258, 5746–5753 (1983)

    PubMed  CAS  Google Scholar 

  6. Lee, M.H.; Gautam-Basak, M.; Wooley, C; Sander, E.G.: Deoxycytidylate hydroxymethylase: purification, properties, and the role of a thiol group in catalysis. Biochemistry, 27, 1367–1373 (1988)

    Article  PubMed  CAS  Google Scholar 

  7. North, T.W.; Mathews, C.K.: T4 phage-coded deoxycytidylate hydroxymethylase: purification and studies on intermolecular interactions. Biochem. Biophys. Res. Commun., 77, 898–904 (1977)

    Article  PubMed  CAS  Google Scholar 

  8. Subramaniam, R.; Wang, Y.; Mathews, C.K.; Santi, D.V.: On the inhibition of deoxycytidylate hydroxymethylase by 5-fluoro-2-deoxycytidine 5-mono-phosphate. Arch. Biochem. Biophys., 275, 11–15 (1989)

    Article  PubMed  CAS  Google Scholar 

  9. Mathews, C.K.; Wheeler, L.J.; Ungermann, C; Young, J.P.; Ray, N.B.: Enzyme interactions involving T4 phage-coded thymidylate synthase and deoxycytidylate hydroxymethylase. Adv. Exp. Med. Biol., 338, 563–570 (1993)

    PubMed  CAS  Google Scholar 

  10. Graves, K.L.; Hardy, L.W.: Kinetic and equilibrium α-secondary tritium isotope effects on reactions catalyzed by dCMP hydroxymethylase from bacteriophage T4. Biochemistry, 33, 13049–13056 (1994)

    Article  PubMed  CAS  Google Scholar 

  11. Butler, M.M.; Graves, K.L.; Hardy, L.W.: Evidence from 18O exchange studies for an exocyclic methylene intermediate in the reaction catalyzed by T4 deoxycytidylate hydroxymethylase. Biochemistry, 33, 10521–10526 (1994)

    Article  PubMed  CAS  Google Scholar 

  12. Hardy, L.W.; Graves, K.L.; Nalivaika, E.: Electrostatic guidance of catalysis by a conserved glutamic acid in Escherichia coli dTMP synthase and bac-teriophage T4 dCMP hydroxymethylase. Biochemistry, 34, 8422–8432 (1995)

    Article  PubMed  CAS  Google Scholar 

  13. Song, H.K.; Sohn, S.H.; Suh, S.W.: Crystal structure of deoxycytidylate hydroxymethylase from bacteriophage T4, a component of the deoxyribonu-cleoside triphosphate-synthesizing complex. EMBO J., 18, 1104–1113 (1999)

    Article  PubMed  CAS  Google Scholar 

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© 2006 Springer-Verlag Berlin Heidelberg

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(2006). Deoxycytidylate 5-hydroxymethyltransferase. In: Schomburg, D., Schomburg, I., Chang, A. (eds) Springer Handbook of Enzymes. Springer Handbook of Enzymes, vol 29. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-37716-6_8

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