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Dihydrolipoamide S-acetyltransferase

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Springer Handbook of Enzymes

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References

  1. Ali, S.T.; Guest, J.R.: Isolation and characterization of lipoylated and unlipoylated domains of the E2p subunit of the pyruvate dehydrogenase complex of Escherichia coli. Biochem. J., 271, 139–145 (1990)

    PubMed  CAS  Google Scholar 

  2. Dardel, R; Packman, L.C.; Perham, R.N.: Expression in Escherichia coli of a sub-gene encoding the lipoyl domain of the pyruvate dehydrogenase complex of Bacillus stearothermophilus [published erratum appears in FEBS Lett 1990 Jul 30;268(l):306]. FEBS Lett., 264, 206–210 (1990)

    Article  PubMed  CAS  Google Scholar 

  3. Oppermann, F.B.; Schmidt, B.; Steinbuchel, A.: Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system. J. Bacteriol., 173, 757–767 (1991)

    PubMed  CAS  Google Scholar 

  4. Niu, X.D.; Browning, K.S.; Behal, R.H.; Reed, L.J.: Cloning and nucleotide sequence of the gene for dihydrolipoamide acetyltransferase from Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA, 85, 7546–7550 (1988)

    Article  PubMed  CAS  Google Scholar 

  5. Hanemaaijer, R.; Westphal, A.H.; Berg, A.; van Dongen, W.; de Kok, A.; Veeger, C: The gene encoding dihydrolipoyl transacetylase from Azotobacter vinelandii. Expression in Escherichia coli and activation and isolation of the protein. Eur. J. Biochem., 181, 47–53 (1989)

    Article  PubMed  CAS  Google Scholar 

  6. Hanemaaijer, R.; Janssen, A.; de Kok, A.; Veeger, C: The dihydrolipoyltransacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Molecular cloning and sequence analysis. Eur. J. Biochem., 174, 593–599 (1988)

    Article  PubMed  CAS  Google Scholar 

  7. Hanemaaijer, R.; Westphal, A.H.; van der Heiden, T.; de Kok, A.; Veeger, C: The quaternary structure of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii. A reconsideration. Eur. J. Biochem., 179, 287–292 (1989)

    Article  PubMed  CAS  Google Scholar 

  8. Hanemaaijer, R.; de Kok, A.; Jolles, J.; Veeger, C: The domain structure of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii. Eur. J. Biochem., 169, 245–252 (1987)

    Article  PubMed  CAS  Google Scholar 

  9. Powers-Greenwood, S.L.; Rahmatullah, M.; Radke, G.A.; Roche, T.E.: Separation of protein X from the dihydrolipoyl transacetylase component of the mammalian pyruvate dehydrogenase complex and function of protein X. J. Biol. Chem., 264, 3655–3657 (1989)

    PubMed  CAS  Google Scholar 

  10. Rahmatullah, M.; Gopalakrishnan, S.; Andrews, PC; Chang, C.L.; Radke, G.A.; Roche, T.E.: Subunit associations in the mammalian pyruvate dehydrogenase complex. Structure and role of protein X and the pyruvate dehydrogenase component binding domain of the dihydrolipoyl transacetylase component. J. Biol. Chem., 264, 2221–2227 (1989)

    PubMed  CAS  Google Scholar 

  11. Rahmatullah, M.; Gopalakrishnan, S.; Radke, G.A.; Roche, T.E.: Domain structures of the dihydrolipoyl transacetylase and the protein X components of mammalian pyruvate dehydrogenase complex. Selective cleavage by protease Arg C. J. Biol. Chem., 264, 1245–1251 (1989)

    PubMed  CAS  Google Scholar 

  12. Hanemaaijer, R.; Masurel, R.; Visser, A.J.W.G.; de Kok, A.; Veeger, C: Time-resolved fluorescence studies on the dihydrolipoyl transacetylase (E2) component of the pyruvate dehydrogenase complex from Azotobacter vinelandii. FEBS Lett., 238, 285–288 (1988)

    Article  PubMed  CAS  Google Scholar 

  13. Packman, L.C.; Borges, A.; Perham, R.N.: Amino acid sequence analysis of the lipoyl and peripheral subunit-binding domains in the lipoate acetyltransferase component of the pyruvate dehydrogenase complex from Bacillus stearothermophilus. Biochem. J., 252, 79–86 (1988)

    PubMed  CAS  Google Scholar 

  14. Thekkumkara, T.J.; Jesse, B.W.; Ho, L.; Raefsky, C; Pepin, R.A.; Javed, A.A.; Pons, G.; Patel, M.S.: Isolation of a cDNA clone for the dihydrolipoamide acetyltransferase component of the human liver pyruvate dehydrogenase complex. Biochem. Biophys. Res. Commun., 145, 903–907 (1987)

    Article  PubMed  CAS  Google Scholar 

  15. Matuda, S.; Matuo, S.; Nakano, K.; Saheki, T.: Molecular cloning of cDNA for rat liver lipoate acetyltransferase. A component of pyruvate dehydrogenase complex. Biochem. Biophys. Res. Commun., 142, 953–957 (1987)

    Article  PubMed  CAS  Google Scholar 

  16. Guest, J.R.; Lewis, H.M.; Graham, L.D.; Packman, L.C.; Perham, R.N.: Genetic reconstruction and functional analysis of the repeating lipoyl domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. J. Mol. Biol., 185, 743–754 (1985)

    Article  PubMed  CAS  Google Scholar 

  17. Phelps, A.; Lindsay, J.G.: Mammalian pyruvate dehydrogenase complex binds tightly to the mitochondrial inner membrane. Biochem. Soc. Trans., 14, 893 (1986)

    CAS  Google Scholar 

  18. De Kok, A.; Westphal, A.H.: Hybrid pyruvate dehydrogenase complexes reconstituted from components of the complexes from Escherichia coli and Azotobacter vinelandii. Eur. J. Biochem., 152, 35–41 (1985)

    Article  PubMed  Google Scholar 

  19. Lowe, P.N.; Hodgson, J.A.; Perham, R.N.: Limited proteolysis of the pyruvate dehydrogenase multienzyme complex of Bacillus subtilis. Biochem. J., 225, 249–253 (1985)

    PubMed  CAS  Google Scholar 

  20. Bosma, H.J.; de Kok, A.; van Markwijk, B.W.; Veeger, C: The size of the pyruvate dehydrogenase complex of Azotobacter vinelandii. Association phenomena. Eur. J. Biochem., 140, 273–280 (1984)

    Article  PubMed  CAS  Google Scholar 

  21. Packman, L.C.; Hale, G.; Perham, R.N.: Repeating functional domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. EMBO J., 3, 1315–1319 (1984)

    PubMed  CAS  Google Scholar 

  22. Guest, J.R.; Darlison, M.G.; Spencer, M.E.; Stephens, P.E.: Cloning and sequence analysis of the pyruvate and 2-oxoglutarate dehydrogenase complex genes of Escherichia coli. Biochem. Soc. Trans., 12, 220–223 (1984)

    PubMed  CAS  Google Scholar 

  23. Wu, T.L.; Reed, L.J.: Subunit binding in the pyruvate dehydrogenase complex from bovine kidney and heart. Biochemistry, 23, 221–226 (1984)

    Article  PubMed  CAS  Google Scholar 

  24. Matuda, S.; Shirahama, T.; Saheki, T.; Miura, S.; Mori, M.: Purification and immunochemical studies of pyruvate dehydrogenase complex from rat heart, and cell-free synthesis of lipoamide dehydrogenase, a component of the complex. Biochim. Biophys. Acta, 741, 86–93 (1983)

    PubMed  CAS  Google Scholar 

  25. Seckler, R.; Binder, R.; Bisswanger, H.: Purification and properties of the pyruvate dehydrogenase complex from Salmonella typhimurium and formation of hybrids with the enzyme complex from Escherichia coli. Biochim. Biophys. Acta, 705, 210–217 (1982)

    PubMed  CAS  Google Scholar 

  26. Graupe, K.; Abusaud, M.; Karfunkel, H.; Bisswanger, H.: Reassociation of the pyruvate dehydrogenase complex from Escherichia coli: Kinetic measurements and binding studies by resonance energy transfer. Biochemistry, 21, 1386–1394 (1982)

    Article  PubMed  CAS  Google Scholar 

  27. Danson, M.J.; Porteous, C.E.: Pyruvate dehydrogenase multienzyme complex of Escherichia coli: determination of the Mr of the lipoate acetyltrans-ferase component. FEBS Lett., 133, 112–114 (1981)

    Article  PubMed  CAS  Google Scholar 

  28. Guest, J.R.; Stephens, P.E.: Molecular cloning of the pyruvate dehydrogenase complex genes of Escherichia coli. J. Gen. Microbiol., 121, 277–292 (1980)

    PubMed  CAS  Google Scholar 

  29. Bleile, D.M.; Hackert, MX.; Pettit, F.H.; Reed, L.J.: Subunit structure of dihydrolipoyl transacetylase component of pyruvate dehydrogenase complex from bovine heart. J. Biol. Chem., 256, 514–519 (1981)

    PubMed  CAS  Google Scholar 

  30. Kresze, G.B.; Ronft, H.: Bovine kidney pyruvate dehydrogenase complex. Limited proteolysis and molecular structure of the lipoate acetyltransferase component. Eur. J. Biochem., 112, 589–599 (1980)

    Article  PubMed  CAS  Google Scholar 

  31. Machicao, F.; Wieland, O.H.: Subunit structure of dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex from bovine kidney. Hoppe-Seyler’s Z. Physiol. Chem., 361, 1093–1106 (1980)

    PubMed  CAS  Google Scholar 

  32. Kresze, G.B.; Dietl, B.; Ronft, H.: Mammalian lipoate acetyltransferase: molecular weight determination by gel filtration in the presence of guanidinium chloride. FEBS Lett., 112, 48–50 (1980)

    Article  PubMed  CAS  Google Scholar 

  33. Bleile, D.M.; Munk, P.; Oliver, R.M.; Reed, L.J.: Subunit structure of dihydrolipoyl transacetylase component of pyruvate dehydrogenase complex from Escherichia coli. Proc. Natl. Acad. Sci. USA, 76, 4385–4389 (1979)

    Article  PubMed  CAS  Google Scholar 

  34. Fuller, C.C.; Reed, L.J.; Oliver, R.M.; Hackert, M.L.: Crystallization of a dihydrolipoyl transacetylase-dihydrolipoyl dehydrogenase sub complex and its implications regarding the subunit structure of the pyruvate dehydrogenase complex from Escherichia coli. Biochem. Biophys. Res. Commun., 90, 431–438 (1979)

    Article  PubMed  CAS  Google Scholar 

  35. Danson, M.J.; Hale, G.; Johnson, P.; Perham, R.N.: Molecular weight and symmetry of the pyruvate dehydrogenase multienzyme complex of Escherichia coli. J. Mol. Biol., 129, 603–617 (1979)

    Article  PubMed  CAS  Google Scholar 

  36. Reed, L.J.; Pettit, F.H.; Eley, M.H.; Hamilton, L.; Collins, J.H.; Oliver, R.M.: Reconstitution of the Escherichia coli pyruvate dehydrogenase complex. Proc. Natl. Acad. Sci. USA, 72, 3068–3072 (1975)

    Article  PubMed  CAS  Google Scholar 

  37. Hamada, M.; Otsuka, K.I.; Tanaka, N.; Ogasahara, K.; Koike, K.; Hiraoka, T.; Koike, M.: Purification properties and subunit composition of pig heart lipoate acetyltransferase. J. Biochem., 78, 187–197 (1975)

    PubMed  CAS  Google Scholar 

  38. Butterworth, P.J.; Tsai, S.; Eley, M.H.; Roche, T.E.; Reed, L.J.: A kinetic study of dihydrolipoyl transacetylase from bovine kidney. J. Biol. Chem., 250, 1921–1925 (1975)

    PubMed  CAS  Google Scholar 

  39. Bisswanger, H.; Henning, U.: A new dihydrolipoamide transacetylase in Escherichia coli K12. Biochim. Biophys. Acta, 321, 143–148 (1973)

    PubMed  CAS  Google Scholar 

  40. Linn, T.C.; Pelley, J.W.; Pettit, F.H.; Hucho, R; Randall, D.D.; Reed, L.J.: α-Keto acid dehydrogenase complexes. XV. Purification and properties of the component enzymes of the pyruvate dehydrogenase complexes from bovine kidney and heart. Arch. Biochem. Biophys., 148, 327–342 (1972)

    Article  PubMed  CAS  Google Scholar 

  41. Vogel, 0.; Beikirch, H.; Muller, H.; Henning, U.: The subunit structure of the Escherichia coli K-12 pyruvate dehydrogenase complex. The dihydrolipoamide transacetylase component. Eur. J. Biochem., 20, 169–178 (1971)

    Article  PubMed  CAS  Google Scholar 

  42. Schwartz, E.R.; Reed, L.J.: α-Keto acid dehydrogenase complexes. XII. Effects of acetylation on the activity and structure of the dihydrolipoyl transacetylase of Escherichia coli. J. Biol. Chem., 244, 6074–6079 (1969)

    PubMed  CAS  Google Scholar 

  43. Schwartz, E.; Reed, L.J.: α-Keto acid dehydrogenase complexes. IX. Effects of iodination of the tyrosyl residues on the properties of the dihydrolipoyl transacetylase of Escherichia coli. J. Biol. Chem., 243, 639–643 (1968)

    PubMed  CAS  Google Scholar 

  44. Henney, H.R.; Willms, C.R.; Muramatsu, T.; Mukherjee, B.B.; Reed, L.J.: α-Keto acid dehydrogenase complexes. VII. Isolation and partial characterization of the polypeptide chains in the dihydrolipoyl transacetylase of Escherichia coli. J. Biol. Chem., 242, 898–901 (1967)

    PubMed  CAS  Google Scholar 

  45. Willms, C.R.; Oliver, R.M.; Henney, H.R.; Mukherjee, B.B.; Reed, L.J.: α-Keto acid dehydrogenase complexes. VI. Dissociation and reconstitution of the dihydrolipoyl transacetylase of Escherichia coli. J. Biol. Chem., 242, 889–897 (1967)

    PubMed  CAS  Google Scholar 

  46. Reed, L.J.; Yeaman, S.J.: Pyruvate dehydrogenase. The Enzymes, 3rd Ed. (Boyer, P.D., ed.), 18, 77–95 (1987)

    Google Scholar 

  47. Harada, T.; Hirabayashi, T.: Pyruvate dehydrogenase complex from Hansenula. Methods Enzymol., 89, 420–423 (1982)

    Article  PubMed  CAS  Google Scholar 

  48. Furuta, S.; Hashimoto, T.: Pyruvate dehydrogenase complex from pigeon breast muscle. Methods Enzymol., 89, 414–420 (1982)

    PubMed  CAS  Google Scholar 

  49. Randall, D.D.: Pyruvate dehydrogenase complex from broccoli and cauliflower. Methods Enzymol., 89, 408–414 (1982)

    CAS  Google Scholar 

  50. Harding, R.W.; Caroline, D.F.; Wagner, R.P.: Pyruvate dehydrogenase complex from Neurospora. Methods Enzymol., 89, 386–391 (1982)

    PubMed  CAS  Google Scholar 

  51. Luderitz, R.; Klemme, J.H.: Isolation and characterization of a membrane-bound pyruvate dehydrogenase complex from the phototropic bacterium Rhodopseudomonas capsulata. Z. Naturforsch. C, 32c, 351–361 (1977)

    Google Scholar 

  52. De Graaf-Hess, A.C.; de Kok, A.: Binding and dissociation of the pyruvate dehydrogenase complex of Azotobacter vinelandii on thiol-Sepharose. FEBS Lett, 143, 261–264 (1982)

    Article  PubMed  Google Scholar 

  53. Borges, A.; Hawkins, C.F.; Packman, L.C.; Perham, R.N.: Cloning and sequence analysis of the genes encoding the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Eur. J. Biochem., 194, 95–102 (1990)

    Article  PubMed  CAS  Google Scholar 

  54. Yeaman, S.J.: The mammalian 2-oxoacid dehydrogenases: a complex family. Trends Biochem. Sci., 11, 293–296 (1986)

    Article  CAS  Google Scholar 

  55. Pettit, F.H.; Reed, L.J.: Pyruvate dehydrogenase complex from bovine kidney and heart. Methods Enzymol., 89, 376–386 (1982)

    PubMed  CAS  Google Scholar 

  56. Henderson, C.E.; Perham, R.N.: Purificaton of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus and resolution of its four component polypeptides. Biochem. J., 189, 161–172 (1980)

    PubMed  CAS  Google Scholar 

  57. Visser, J.; Kester, H.; Huigen, A.: Purification and some properties of the pyruvate dehydrogenase complex of Bacillus subtilis. FEMS Microbiol. Lett., 9, 227–232 (1980)

    Article  CAS  Google Scholar 

  58. Kresze, G.B.; Ronft, H.: Pyruvate dehydrogenase complex from baker’s yeast. 2. Molecular structure, dissociation, and implications for the origin of mitochondria. Eur. J. Biochem., 119, 581–587 (1981)

    Article  PubMed  CAS  Google Scholar 

  59. Ala’ Aldeen, D.A.A.; Westphal, A.H.; De Kok, A.; Weston, V.; Atta, M.S.; Baldwin, T.J.; Bartley, J.; Bordello, S.P.: Cloning, sequencing, characterization and implications for vaccine design of the novel dihydrolipoyl acetyl-transferase of Neisseria meningitidis. J. Med. Microbiol., 45, 419–432 (1996)

    Article  PubMed  CAS  Google Scholar 

  60. Allen, M.D.; Perham, R.N.: The catalytic domain of dihydrolipoyl acetyltransferase from the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus. Expression, purification and reversible denaturation. FEBS Lett., 413, 339–343 (1997)

    Article  PubMed  CAS  Google Scholar 

  61. Aso, Y.; Nakajima, A.; Meno, K.; Ishiguro, M.: Thermally induced changes of lipoate acetyltransferase inner core isolated from the Bacillus stearothermophilus pyruvate dehydrogenase complex. Biosci. Biotechnol. Biochem., 65, 698–701 (2001)

    Article  PubMed  CAS  Google Scholar 

  62. Guan, Y.; Rawsthorne, S.; Scofield, G.; Shaw, P.; Doonan, J.: Cloning and characterization of a dihydrolipoamide acetyltransferase (E2) subunit of the pyruvate dehydrogenase complex from Arabidopsis thaliana. J. Biol. Chem., 270, 5412–5417 (1995)

    Article  PubMed  CAS  Google Scholar 

  63. Hendle, J.; Mattevi, A.; Westphal, A.H.; Spee, J.; de Kok, A.; Teplyakov, A.; Hol, W.G.: Crystallographic and enzymatic investigations on the role of Ser558, His610, and Asn614 in the catalytic mechanism of Azotobacter vinelandii dihydrolipo amide acetyltransferase (E2p). Biochemistry, 34, 4287–4298 (1995)

    Article  PubMed  CAS  Google Scholar 

  64. Jackson, J.C.; Vinluan, C.C.; Dragland, C.J.; Sundararajan, V.; Yan, B.; Gounarides, J.S.; Nirmala, N.R.; Topiol, S.; Ramage, P.; Blume, J.E.; Aicher, T.D.; Bell, P.A.; Mann, W.R.: Heterologously expressed inner lipoyl domain of dihydrolipoyl acetyltransferase inhibits ATP-dependent inactivation of pyruvate dehydrogenase complex, Identification of important amino acid residues. Biochem. J., 334, 703–711 (1998)

    PubMed  CAS  Google Scholar 

  65. Mande, S.S.; Sarfaty, S.; Allen, M.D.; Perham, R.N.; Hol, W.G.J.: Proteinprotein interactions in the pyruvate dehydrogenase multienzyme complex: dihydrolipoamide dehydrogenase complexed with the binding domain of dihydrolipoamide acetyltransferase. Structure, 4, 277–286 (1996)

    Article  PubMed  CAS  Google Scholar 

  66. McCartney, R.G.; Sanderson, S.J.; Lindsay, J.G.: Refolding and reconstitution studies on the transacetylaseprotein X (E2/X) subcomplex of the mammalian pyruvate dehydrogenase complex: Evidence for specific binding of the dihydrolipoamide dehydrogenase component to sites on reassembled E2. Biochemistry, 36, 6819–6826 (1997)

    Article  PubMed  CAS  Google Scholar 

  67. Millar, A.H.; Leaver, C.J.; Hill, S.A.: Characterization of the dihydrolipoamide acetyltransferase of the mitochondrial pyruvate dehydrogenase complex from potato and comparisons with similar enzymes in diverse plant species. Eur. J. Biochem., 264, 973–981 (1999)

    Article  PubMed  CAS  Google Scholar 

  68. Mooney, B.R; Miernyk, J.A.; Randall, D.D.: Cloning and characterization of the dihydrolipoamide S-acetyltransferase subunit of the plastid pyruvate dehydrogenase complex (E2) from Arabidopsis. Plant Physiol., 120, 443–452 (1999)

    Article  PubMed  CAS  Google Scholar 

  69. Oppermann, F.B.; Schmidt, B.; Steinbuchel, A.: Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system. J. Bacteriol., 173, 757–767 (1991)

    PubMed  CAS  Google Scholar 

  70. Roche, T.E.; Powers-Greenwood, S.L.; Shi, W.F.; Zhang, W.B.; Ren, S.Z.; Roche, E.D.; Cox, D.J.; Sorensen, CM.: Sizing of bovine heart and kidney pyruvate dehydrogenase complex and dihydrolipoyl transacetylase core by quasielastic light scattering. Biochemistry, 32, 5629–5637 (1993)

    Article  PubMed  CAS  Google Scholar 

  71. Schulze, E.; Westphal, A.H.; Obmolova, G.; Mattevi, A.; Hol, W.G.; de Kok, A.: The catalytic domain of the dihydrolipoyl transacetylase component of the pyruvate dehydrogenase complex from Azotobacter vinelandii and Escherichia coli. Expression, purification, properties and preliminary X-ray analysis. Eur. J. Biochem., 201, 561–568 (1991)

    Article  PubMed  CAS  Google Scholar 

  72. Schulze, E.; Westphal, A.H.; Veenhuis, M.; de Kok, A.: Purification and cellular localization of wild type and mutated dihydrolipoyltransacetylases from Azotobacter vinelandii and Escherichia coli expressed in E. coli. Biochim. Biophys. Acta, 1120, 87–96 (1992)

    PubMed  CAS  Google Scholar 

  73. Thelen, J.J.; Muszynski, M.G.; David, N.R.; Luethy, M.H.; Elthon, T.E.; Miernyk, J.A.; Randall, D.D.: The dihydrolipoamide S-acetyltransferase subunit of the mitochondrial pyruvate dehydrogenase complex from maize contains a single lipoyl domain. J. Biol. Chem., 274, 21769–21775 (1999)

    Article  PubMed  CAS  Google Scholar 

  74. Wang, L.; Kaneko, S.; Kagaya, M.; Ohno, H.; Honda, M.; Kobayashi, K.: Molecular cloning, and characterization and expression of dihydrolipoamide acetyltransferase component of murine pyruvate dehydrogenase complex in bile duct cancer cells. J. Gastroenterol., 37, 449–454 (2002)

    Article  PubMed  CAS  Google Scholar 

  75. Yang, D.; Song, J.; Wagenknecht, T.; Roche, T.E.: Assembly and full functionality of recombinantly expressed dihydrolipoyl acetyltransferase component of the human pyruvate dehydrogenase complex. J. Biol. Chem., 272, 6361–6369 (1997)

    Article  PubMed  CAS  Google Scholar 

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(2006). Dihydrolipoamide S-acetyltransferase. In: Schomburg, D., Schomburg, I., Chang, A. (eds) Springer Handbook of Enzymes. Springer Handbook of Enzymes, vol 29. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-37716-6_41

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