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Functional genetics of Yarrowia lipolytica

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Functional Genetics of Industrial Yeasts

Part of the book series: Topics in Current Genetics ((TCG,volume 2))

Abstract

The yeast Yarrowia lipolytica has been used for industrial bioconversions since the late 1960’s, mainly by the food and chemical industries. The most important process is presently the production of citric acid from rapeseed oil, but Y. lipolytica has also been considered for various other applications, ranging from single cell protein to heterologous proteins production. Several of these procedures have received a GRAS status, which facilitates further applications of this yeast in the food and pharmaceutical industries. This review focuses on the acquisition of basic knowledge on Y. lipolytica, which is physiologically and taxonomically very distant from Saccharomyces cerevisiae. Thanks to the development of advanced genetic tools for both classical approaches and recombinant DNA technology, significant progresses have been achieved in several aspects of its unusual biology. Particular attention has been paid to the study of early steps of protein secretion, peroxisome biogenesis, carbon metabolism and utilisation of hydrophobic substrates, mitochondrial biology, morphogenetic control, and proteae regulation. An international effort relayed by the Genoscope will permit access to its full genome sequence by the end of 2003, thus facilitating further developments of its functional genetics.

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References

  • Ahlers PM, Garofano A, Kerscher SJ, Brandt U (2000) Application of the obligate aerobic yeast Yarrowia lipolytica as a eucaryotic model to analyse Leigh syndrome mutations in the complex I core subunits PSST and TYKY. Biochim Biophys Acta 1459:258–65.

    Article  CAS  Google Scholar 

  • Albracht SP (1993) Intimate relationships of the large and the small subunits of all nickel hydrogenases with two nuclear-encoded subunits of mitochondrial NADH:ubiquinone oxidoreductase. Biochim Biophys Acta 1144:221–224

    Article  CAS  Google Scholar 

  • Arthur H, Watson K (1976) Thermal adaptation in yeast:growth temperatures, membrane lipid, and cytochrome composition of psychrophilic, mesophilic, and thermophilic yeasts. J Bacteriol 128:56–68

    CAS  Google Scholar 

  • Arzumanov TE, Shishkanova NV, Finogenova TV (2000) Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol. Appl Microbiol Biotechnol 53:525–529

    Article  CAS  Google Scholar 

  • Augstein A, Barth K, Gentsch M, Kohlwein SD, Barth G (2003) Characterisation, localisation and functional analysis of Gprlp, a protein effecting sensitivity to acetic acid in the yeast Yarrowia lipolytica. Microbiology In press

    Google Scholar 

  • Bakker BM, Bro C, Kotter P, Luttik MA, van Dijken JP, Pronk JT (2000) The mitochondrial alcohol dehydrogenase Adh3p is involved in a redox shuttle in Saccharomyces cerevisiae. J Bacteriol 182:4730–4737

    Article  CAS  Google Scholar 

  • Barns SM, Lane DJ, Sogin ML, Bibeau C, Weisburg WG (1991) Evolutionary relationships among pathogenic Candida species and relatives. J Bacteriol 173:2250–2255

    CAS  Google Scholar 

  • Barth G, Gaillardin C (1996) Yarrowia lipolytica. In:Wolf K (ed) Non-Conventional Yeasts in Biotechnology. A Handbook Springer Verlag, Heidelberg, Germany, pp 313–388

    Google Scholar 

  • Barth G, Gaillardin C (1997) Physiology and genetics of the dimorphic fungus Yarrowia lipolytica. FEMS Microbiol Rev 19:219–237

    Article  CAS  Google Scholar 

  • Barth G, Kunkel W (1979) Alcohol dehydrogenase (ADH) in yeasts. II NAD+-and NADP+-dependent alcohol dehydrogenase s in Saccharomycopsis lipolytica. Z Allg Mikrobiol 19:381–390

    Article  CAS  Google Scholar 

  • Barth G, Scheuber T (1993) Cloning of the isocitrate lyase gene (ICL1) from Yarrowia lipolytica and characterization of the deduced protein. Mol Gen Genet 241:422–430

    Article  CAS  Google Scholar 

  • Barth G, Weber H (1985) Improvement of sporulation in the yeast Yarrowia lipolytica. Antonie Van Leeuwenhoek 51:167–177

    Article  CAS  Google Scholar 

  • Barth G, Weber H (1987) Genetic analysis of the gene ICL1 of the yeast Yarrowia lipolytica. Yeast 3:255–262

    Article  CAS  Google Scholar 

  • Bassel J, Mortimer RK (1985) Identification of mutations preventing n-hexadecane uptake among non-utilizing mutants of Yarrowia lipolytica. Curr Genet 9:579–586

    Article  CAS  Google Scholar 

  • Bassel JB, Mortimer EK (1982) Genetic and biochemical studies on n-alkane non-utilizing mutants of Saccharomycopsis lipolytica. Curr Genet 5:77–88

    Article  CAS  Google Scholar 

  • Berninger G, Schmidtchen R, Casel G, Knorr A, Rautenstrauss K, Kunau WH, Schweizer E (1993) Structure and metabolic control of the Yarrowia lipolytica peroxisomal 3-oxoacyl-CoA-thiolase gene. Eur J Biochem 216:607–613

    Article  CAS  Google Scholar 

  • Blazquez MA, Gancedo JM, Gancedo C (1994) Use of Yarrowia lipolytica hexokinase for the quantitative determination of trehalose 6-phosphate. FEMS Microbiol Lett 121:223–227

    Article  CAS  Google Scholar 

  • Boisrame A, Beckerich JM, Gaillardin C (1996) Slslp, an endoplasmic reticulum component, is involved in the protein translocation process in the yeast Yarrowia lipolytica. J Biol Chem 271:11668–11675

    Article  CAS  Google Scholar 

  • Boisrame A, Beckerich JM, Gaillardin C (1999) A mutation in the secretion pathway of the yeast Yarrowia lipolytica that displays synthetic lethality in combination with a mutation affecting the signal recognition particle. Mol Gen Genet 261:601–609

    Article  CAS  Google Scholar 

  • Boisrame A, Chasles M, Babour A, Beckerich JM, Gaillardin C (2002) Sbhlp, a subunit of the Sec61 translocon, interacts with the chaperone calnexin in the yeast Yarrowia lipolytica. J Cell Sci 115:4947–4956

    Article  CAS  Google Scholar 

  • Boisrame A, Kabani M, Beckerich JM, Hartmann E, Gaillardin C (1998) Interaction of Kar2p and Slslp is required for efficient co-translational translocation of secreted proteins in the yeast Yarrowia lipolytica. JBiol Chem 273:30903–30908

    Article  CAS  Google Scholar 

  • Bon E, Casaregola S, Blandin G, Llorente B, Neuveglise C, Munsterkotter M, Guldener U, Mewes HW, Van Helden J, Dujon B, Gaillardin C (2003) Molecular Evolution of Eukaryotic Genomes:Hemiascomycetous Yeast Spliceosomal Introns. Nucl Acids Res 31:in press.

    Google Scholar 

  • Brandt U (1997) Proton-translocation by membrane-bound NADH:ubiquinone-oxidoreductase (complex I) through redox-gated ligand conduction. Biochim Biophys Acta 1318:79–91

    Article  CAS  Google Scholar 

  • Brandt U (1999) Proton translocation in the respiratory chain involving ubiquinone-a hypothetical semiquinone switch mechanism for complex I. Biofactors 9:95–101

    CAS  Google Scholar 

  • Broughton J, Swennen D, Wilkinson BM, Joyet P, Gaillardin C, Stirling CJ (1997) Cloning of SEC61 homologues from Schizosaccharomyces pombe and Yarrowia lipolytica reveals the extent of functional conservation within this core component of the ER translocation machinery. J Cell Sci 110:2715–2727

    CAS  Google Scholar 

  • Bruyn J (1954) An intermediate product in the oxidation of hexadecene-1 by Candida lipolytica. Koninkl. Ned. Acad. Wetenschap. Proc Ser C 57:41–44

    Google Scholar 

  • Buschges R, Bahrenberg G, Zimmermann M, Wolf K (1994) NADH:ubiquinone oxidoreduetase in obligate aerobic yeasts. Yeast 10:475–479

    Article  CAS  Google Scholar 

  • Cardillo R, Fronza G, Fuganti C, Grasseli P, Mele A, Pizzi D (1991) Stereochemistry of the microbial generation of delta-decanolide, gamma-dodecanolide and gamma-nonanolide from C18 13-hydroxy, C18 10-hydroxy, and C19 14-hydroxy unsaturated fatty acids. J Org Chem 56:5237–5239

    Article  CAS  Google Scholar 

  • Casaregola S, Feynerol C, Diez M, Fournier P, Gaillardin C (1997) Genomic organization of the yeast Yarrowia lipolytica. Chromosoma 106:380–390

    Article  CAS  Google Scholar 

  • Casaregola S, Neuveglise C, Bon E, Gaillardin C (2002) Ylli, a non-LTR retrotransposon LI family in the dimorphic yeast Yarrowia lipolytica. Mol Biol Evol 19:664–677

    CAS  Google Scholar 

  • Casaregola S, Neuveglise C, Lepingle A, Bon E, Feynerol C, Artiguenave F, Wincker P, Gaillardin C (2000) Genomic exploration of the hernias corny cetous yeasts:17. Yarrowia lipolytica. FEBS Lett 487:95–100

    Article  CAS  Google Scholar 

  • Chen DC, Beckerich JM, Gaillardin C (1997a) One-step transformation of the dimorphic yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 48:232–235

    Article  CAS  Google Scholar 

  • Chen S, Brockenbrough JS, Dove JE, Aris JP (1997b) Homocitrate synthase is located in the nucleus in the yeast Saccharomyces cerevisiae. J Biol Chem 272:10839–10846

    Article  CAS  Google Scholar 

  • Chernyavskaya OG, Shishkanova NV, Il’chenko AP, Finogenova TV (2000) Synthesis of alpha-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol. Appl Microbiol Biotechnol 53:152–158

    Article  CAS  Google Scholar 

  • Cirigliano MC, Carman GM (1984) Isolation of a bioemulsifier from Candida lipolytica. Appl Environ Microbiol 48:747–750

    CAS  Google Scholar 

  • Cirigliano MC, Carman GM (1985) Purification and characterization of liposan, a bioemulsifier from Candida lipolytica. Appl Environ Microbiol 50:846–850

    CAS  Google Scholar 

  • Cordero Otero R, Gaillardin C (1996) Efficient selection of hygromycin-B-resistant Yarrowia lipolytica transformants. Appl Microbiol Biotechnol 46:143–148

    Article  CAS  Google Scholar 

  • Darrouzet E, Issartel JP, Lunardi J, Dupuis A (1998) The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of piericidin and rotenone, two quinone-related inhibitors. FEBS Lett 431:34–38

    Article  CAS  Google Scholar 

  • De Duve C, Baudhuin P (1966) Peroxisomes (microbodies and related particles). Physiol Rev 46:323–357

    Google Scholar 

  • de Vries S, Grivell LA (1988) Purification and characterization of a rotenone-insensitive NADH:Q6 oxidoreductase from mitochondria of Saccharomyces cerevisiae. Eur J Biochem 176:377–384

    Article  Google Scholar 

  • De Vries S, Van Witzenburg R, Grivell LA, Marres CA (1992) Primary structure and import pathway of the rotenone-insensitive NADH-ubiquinone oxidoreductase of mitochondria from Saccharotnyces cerevisiae. Eur J Biochem 203:587–592

    Article  Google Scholar 

  • Delaisse JM, Martin P, Verheyen-Bouvy MF, Nyns EJ (1981) Subcellular distribution of enzymes in the yeast Saccharomycopsis lipolytica, grown on n-hexadecane, with special reference to the omega-hydroxylase. Biochim Biophys Acta 676:77–90

    CAS  Google Scholar 

  • Dell’Angelica EC, Ermacora MR, Santome JA(1996) Purification and partial characterization of a fatty acid-binding protein from the yeast, Yarrowia lipolytica. Biochem Mol Biol Int 39:439–445

    CAS  Google Scholar 

  • Dell’Angelica EC, Stella CA, Ermacora MR, Ramos EH, Santome JA (1992) Study on fatty acid binding by proteins in yeast. Dissimilar results in Saccharotnyces cerevisiae and Yarrowia lipolytica. Comp Biochem Physiol B 102:261–265

    Article  CAS  Google Scholar 

  • Djafarzadeh R, Kerscher S, Zwicker K, Radermacher M, Lindahl M, Schagger H, Brandt U (2000) Biophysical and structural characterization of proton-translocating NADH-dehydrogenase (complex I) from the strictly aerobic yeast Yarrowia lipolytica. Biochim Biophys Acta 1459:230–238

    Article  CAS  Google Scholar 

  • Dominguez A, Ferminan E, Sanchez M, Gonzalez FJ, Perez-Campo FM, Garcia S, Herrero AB, San Vicente A, Cabello J, Prado M, Iglesias FJ, Choupina A, Burguillo FJ, Fernandez-Lago L, Lopez MC (1998) Non-conventional yeasts as hosts for heterologous protein production. Int Mi crobiol 1:131–142

    CAS  Google Scholar 

  • Ellis RJ, Haiti FU (1999) Principles of protein folding in the cellular environment. Curr Opin Struct Biol 9:102–110

    Article  CAS  Google Scholar 

  • Ercoli B, Fuganti C, Grasselli P, Servi S, Allegrone G (1992) Stereochemistry of the biogeneration of C-10 and C-12 gamma lactones in Yarrowia lipolytica and Pichia ohtnen. Biotechnol Lett 14:665–668

    Article  CAS  Google Scholar 

  • Fearnley IM, Carroll J, Shannon RJ, Runswick MJ, Walker JE, Hirst J (2001) GRIM-19, a cell death regulatory gene product, is a subunit of bovine mitochondrial NADH:ubiquinone oxidoreductase (complex I). J Biol Chem 276:38345–38348

    Article  CAS  Google Scholar 

  • Finel M (1998) Organization and evolution of structural elements within complex I. Biochim Biophys Acta 1364:112–121

    Article  CAS  Google Scholar 

  • Finogenova TV, Kamzolova SV, Dedyukhina EG, Shishkanova NV, H’chenko AP, Morgunov IG, Chernyavskaya OG, Sokolov AP (2002) Biosynthesis of citric and isocitric acids from ethanol by mutant Yarrowia lipolytica N 1 under continuous cultivation. Appl Microbiol Biotechnol 59:493–500

    Article  CAS  Google Scholar 

  • Flores CL, Rodriguez C, Petit T, Gancedo C (2000) Carbohydrate and energy-yielding metabolism in non-conventional yeasts. FEMS Microbiol Rev 24:507–529

    CAS  Google Scholar 

  • Förster A (2001) Untersuchungen zur heterologen Expression von steroidwandelnden Cytochrom P450 Systemen in der Hefe Yarrowia lipolytica. Diploma thesis, Dresden Technical University

    Google Scholar 

  • Fournier P, Abbas A, Chasles M, Kudla B, Ogrydziak DM, Yaver D, Xuan JW, Peito A, Ribet AM, Feynerol C, et al. (1993) Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica. Proc Natl Acad Sci USA 90:4912–4916

    Article  CAS  Google Scholar 

  • Fournier P, Gaillardin C, Persuy MA, Klootwijk J, van Heerikhuizen H (1986) Heterogeneity in the ribosomal family of the yeast Yarrowia lipolytica:gtnormc organization and segregation studies. Gene 42:273–282

    Article  CAS  Google Scholar 

  • Fournier P, Guyaneux L, Chasles M, Gaillardin C (1991) Scarcity of ars sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytica. Yeast 7:25–36

    Article  CAS  Google Scholar 

  • Friedrich T, Scheide D (2000) The respiratory complex I of bacteria, archaea and eukarya and its module common with membrane-bound multisubunit hydrogenases. FEBS Lett 479:1–5

    Article  CAS  Google Scholar 

  • Gaillardin C, Heslot H (1979) Evidence for mutations in the structural gene for homocitrate synthase in Saccharomycopsis lipolytica. Mol Gen Genet 172:185–192

    Article  CAS  Google Scholar 

  • Gimeno CJ, Fink GR (1994) Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol Cell Biol 14:2100–2112

    CAS  Google Scholar 

  • Gonzalez-Lopez CI, Szabo R, Blanchin-Roland S, Gaillardin C (2002) Genetic control of extracellular protease synthesis in the yeast Yarrowia lipolytica. Genetics 160:417–427

    CAS  Google Scholar 

  • Goodwin TJ, Ormandy JE, Poulter RT (2001) Ll-like non-LTR retrotransposons in the yeast Candida albicans. Curr Genet 39:83–91

    Article  CAS  Google Scholar 

  • Guerzoni ME, Lanciotti R, Vannini L, Galgano F, Favati F, Gardini F, Suzzi G (2001) Variability of the lipolytic activity in Yarrowia lipolytica and its dependence on environmental conditions. Int J Food Microbiol 69:79–89.

    Article  CAS  Google Scholar 

  • Hagihara T, Mishina M, Tanaka A, Fukui S (1977) Utilization of pristane by the yeast Candida lipolytica. Fatty acid composition of pristane-grown cells. Agric Biol Chem 41:1745–1748

    CAS  Google Scholar 

  • He F, Beckerich JM, Gaillardin C (1992) A mutant of 7SL RNA in Yarrowia lipolytica affecting the synthesis of a secreted protein. J Biol Chem 267:1932–1937

    CAS  Google Scholar 

  • Henry MF, Verheyen-Bouvy MF, Nyns EJ (1978) Biogenesis of coupling site I in the yeast Saccharomycopsis lipolytica. Arch Int Physiol Biochim 86:862–863.

    CAS  Google Scholar 

  • Herrero AB, Lopez MC, Fernandez-Lago L, Dominguez A (1999) Candida albicans and Yarrowia lipolytica as alternative models for analysing budding patterns and germ tube formation in dimorphic fungi. Microbiology 145:2727–2737

    CAS  Google Scholar 

  • Heslot H (1990) Genetics and genetic engineering of the industrial yeast Yarrowia lipolytica. Adv Biochem Eng Biotechnol 43:43–73

    CAS  Google Scholar 

  • Hirai M, Ohtani E, Tanaka A, Fukui S (1977) Glucose-phosphorylating enzymes of Candida yeasts and their regulation in vivo. Biochim Biophys Acta 480:357–366

    CAS  Google Scholar 

  • Hoenes I, Simon M, Weber H, lipolytica. Coilfty Y (1991) Characterization of isocitrate lyase from the yeast Yarrowia lipolytica. J Basic Microbiol 31:251–258

    Article  CAS  Google Scholar 

  • Hull CM, Johnson AD (1999) Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans. Science 285:1271–1275

    Article  CAS  Google Scholar 

  • Hurtado CA, Beckerich JM, Gaillardin C, Rachubinski RA (2000) A rac homolog is required for induction of hyphal growth in the dimorphic yeast Yarrowia lipolytica. J Bacteriol 182:2376–2386

    Article  CAS  Google Scholar 

  • Hurtado CA, Rachubinski RA (1999) MHY1 encodes a C2H2-type zinc finger protein that promotes dimorphic transition in the yeast Yarrowia lipolytica. J Bacteriol 181:3051–3057

    CAS  Google Scholar 

  • Hurtado CA, Rachubinski RA (2002a) Isolation and characterization of YlBEM1, a gene required for cell polarization and differentiation in the dimorphic yeast Yarrowia lipolytica. Eukaryot Cell 1:526–537

    Article  CAS  Google Scholar 

  • Hurtado CA, Rachubinski RA (2002b) YlBMH1 encodes a 14-3-3 protein that promotes filamentous growth in the dimorphic yeast Yarrowia lipolytica. Microbiology 148:3725–3735

    CAS  Google Scholar 

  • Iida T, Ohta A, Takagi M (1998) Cloning and characterization of an n-alkane-inducible cytochrome P450 gene essential for n-decane assimilation by Yarrowia lipolytica. Yeast 14:1387–1397

    Article  CAS  Google Scholar 

  • Iida T, Sumita T, Ohta A, Takagi M (2000) The cytochrome P450ALK multigene family of an n-alkane-assimilating yeast, Yarrowia lipolytica: cloning and characterization of genes coding for new CYP52 family members. Yeast 16:1077–1087

    Article  CAS  Google Scholar 

  • Il’chenko AP, Cherniavskaia OG, Shishkanova NV, Finogenova TV (2001) [Metabolic characteristics of the Yarrowia lipolytica strain producing alpha-ketoglutaric and citric acids from ethanol and the effect of [NH4+] and [02] on yeast respiration and biosynthesis]. Mikrobiologiia 70:189–195

    CAS  Google Scholar 

  • H’chenko AP, Mauersberger S, Matyashova RN, Lozinov ABM, 452–458. (1980) Induction of cytochrome P-450 in the course of yeast growth on different substrates. Mikrobiologija 49:452–458

    Google Scholar 

  • Ishikura T, Forster JW (1961) Incorporation of molecular oxygen during microbial utilization of olefins. Nature 192:892–893

    Article  CAS  Google Scholar 

  • Jimenez-Bremont JF, Ruiz-Herrera J, A. D (2001) Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica. FEMS Yeast Research 1:195–204

    Article  CAS  Google Scholar 

  • Joseph-Horne T, Hollomon DW, Wood PM (2001) Fungal respirations fusion of standard and alternative components. Biochim Biophys Acta 1504:179–195

    Article  CAS  Google Scholar 

  • Juretzek T (2000) Comparison of promoters suitable for regulated overexpression of b-galactosidase in the alkane-utilizing yeast Yarrowia lipolytica. Biotechnol Bioprocess Eng 5:320–326

    Article  CAS  Google Scholar 

  • Juretzek T, Le Dall M, Mauersberger S, Gaillardin C, Barth G, Nicaud J (2001) Vectors for gene expression and amplification in the yeast Yarrowia lipolytica. Yeast 18:97–113

    Article  CAS  Google Scholar 

  • Kabani M, Beckerich JM, Brodsky JL (2002) Nucleotide exchange factor for the yeast Hsp70 molecular chaperone Ssalp. Mol Cell Biol 22:4677–4689

    Article  CAS  Google Scholar 

  • Kabani M, Beckerich JM, Gaillardin C (2000) Slslp stimulates Sec63p-mediated activation of Kar2p in a conformation-dependent manner in the yeast endoplasmic reticulum. Mol Cell Biol 20:6923–6934

    Article  CAS  Google Scholar 

  • Kamiryo T, Nishikawa Y, Mishina M, Terao M, Numa S (1979) Involvement of long-chain acyl coenzyme A for lipid synthesis in repression of acetyl-coenzyme A carboxylase in Candida lipolytica. Proc Natl Acad Sci USA 76:4390–4394

    Article  CAS  Google Scholar 

  • Kashani-Poor N, Kerscher S, Zickermann V, Brandt U (2001a) Efficient large scale purification of his-tagged proton translocating NADH:ubiquinone oxidoreductase (complex I) from the strictly aerobic yeast Yarrowia lipolytica. Biochim Biophys Acta 1504:363–370

    Article  CAS  Google Scholar 

  • Kashani-Poor N, Zwicker K, Kerscher S, Brandt U (2001b) A central functional role for the 49-kDa subunit within the catalytic core of mitochondrial complex I. J Biol Chem 276:24082–24087

    Article  CAS  Google Scholar 

  • Kemp GD, Dickinson FM, Ratledge C (1990) Light sensitivity of the n-alkane-induced fatty alcohol oxidase from Candida tropicahs and Yarrowia lipolytica. Appl Microbiol Biotechnol 32:461–464

    Article  CAS  Google Scholar 

  • Kerscher S, Drose S, Zwicker K, Zickermann V, Brandt U (2002) Yarrowia lipolyticaf a yeast genetic system to study mitochondrial complex I. Biochim Biophys Acta 1555:83–91

    Article  CAS  Google Scholar 

  • Kerscher S, Durstewitz G, Casaregola S, Gaillardin C, Brandt U (2001a) The complete mitochondrial genome of Yarrowia lipolytica. Comp. Funct Genom 2:80–90

    Article  CAS  Google Scholar 

  • Kerscher S, Kashani-Poor N, Zwicker K, Zickermann V, Brandt U (2001b) Exploring the catalytic core of complex I by Yarrowia lipolytica yeast genetics. J Bioenerg Biomembr 33:187–196

    Article  CAS  Google Scholar 

  • Kerscher SJ (2000) Diversity and origin of alternative NADH:ubiquinone oxidoreductases. Biochim Biophys Acta 1459:274–283

    Article  CAS  Google Scholar 

  • Kerscher SJ, Eschemann A, Okun PM, Brandt U (2001c) External alternative NADH:ubiquinone oxidoreductase redirected to the internal face of the mitochondrial inner membrane rescues complex I deficiency in Yarrowia lipolytica. J Cell Sci 114:3915–3921

    CAS  Google Scholar 

  • Kerscher SJ, Okun JG, Brandt U (1999) A single external enzyme confers alternative NADH:ubiquinone oxidoreductase activity in Yarrowia lipolytica. J Cell Sci 112:2347–2354

    CAS  Google Scholar 

  • Kim J, Cheon SA, Park S, Song Y, Kim JY (2000) Serum-induced hypha formation in the dimorphic yeast Yarrowia lipolytica. FEMS Microbiol Lett 190:9–12

    Article  CAS  Google Scholar 

  • Kim K, Ogrydziak DM (2000) Yarrowia lipolytica SEP receptor alpha-subunit. Yeast 16:1243–1252

    Article  CAS  Google Scholar 

  • Kitano H, Sekito T, Ishitomi H, Yoshida K (1995) The distribution of ND genes in yeast mitochondrial genomes and the mitochondrial DNA structure of Pichia membranae-facens. Nucleic Acids Symp Ser 34:23–24

    CAS  Google Scholar 

  • Klug MJ, Markovetz AJ (1967) Degradation of hydrocarbons by members of the genus Candida. II. Oxidation of n-alkanes and 1-alkenes by Candida lipolytica. J Bacteriol 93:1847–1852

    CAS  Google Scholar 

  • Kohlwein SD, Paltauf F (1984) Uptake of fatty acids by the yeasts, Saccharotnyces uvarum and Sacchar omycopsis lipolytica. Biochim Biophys Acta 792:310–317

    CAS  Google Scholar 

  • Kottig H, Rottner G, Beck KF, Schweizer M, Schweizer E (1991) The pentafunctional FAS1 genes of Saccharotnyces cerevisiae and Yarrowia lipolytica are co-linear and considerably longer than previously estimated. Mol Gen Genet 226:310–314

    Article  CAS  Google Scholar 

  • Kujau M, Weber H, Barth G (1992) Characterization of mutants of the yeast Yarrowia lipolytica defective in acetyl-coenzyme A synthetase. Yeast 8:193–203

    Article  CAS  Google Scholar 

  • Kurischko C, Schilhabel MB, Kunze I, Franzi E (1999) The MATA locus of the dimorphic yeast Yarrowia lipolytica consists of two divergently oriented genes. Mol Gen Genet 262:180–188

    Article  CAS  Google Scholar 

  • Lazarow PB, Fujiki Y (1985) Biogenesis of peroxisomes. Annu Rev Cell Biol 1:489–530

    Article  CAS  Google Scholar 

  • Le Dall M, Nicaud J, Treton BY, Gaillardin CM (1996) The 3-phosphogly cerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates. Curr Genet 29:446–456

    Article  Google Scholar 

  • Le Dali MT, Nicaud JM, Gaillardin C (1994) Multiple-copy integration in the yeast Yarrowia lipolytica. Curr Genet 26:38–44

    Article  Google Scholar 

  • Lee IH, Ogrydziak DM (1997) Yarrowia lipolytica SRP54 homolog and translocation of Kar2p. Yeast 13:499–513

    Article  CAS  Google Scholar 

  • Lengeler KB, Davidson RC, D’Souza C, Harashima T, Shen WC, Wang P, Pan X, Waugh M, Heitman J (2000) Signal transduction cascades regulating fungal development and virulence. Microbiol Mol Biol Rev 64:746–785

    Article  CAS  Google Scholar 

  • Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, Darnell J (1995) Molecular Cell Biology. Peroxysome biosynthesis. W.H. Freeman and Co., New York, pp 837–839

    Google Scholar 

  • Loeffen J, Smeitink J, Triepels R, Smeets R, Schuelke M, Sengers R, Trijbels F, Hamel B, Mullaart R, van den Heuvel L (1998) The first nuclear-encoded complex I mutation in a patient with Leigh syndrome. Am J Hum Genet 63:1598–608

    Article  CAS  Google Scholar 

  • Lopez MC, Nicaud JM, Skinner HB, Vergnolle C, Kader JC, Bankaitis VA, Gaillardin C (1994) A phosphatidylinositol/phosphatidylcholine transfer protein is required for differentiation of the dimorphic yeast Yarrowia lipolytica from the yeast to the mycelial form. J Cell Biol 125:113–127

    Article  CAS  Google Scholar 

  • Luo YS, Nicaud JM, Van Veldhoven PP, Chardot T (2002) The acyl-CoA oxidases from the yeast Yarrowia lipolytica: characterization of Aox2p. Arch Biochem Biophys 407:32–38

    Article  CAS  Google Scholar 

  • Luo YS, Wang HJ, Gopalan KV, Srivastava DK, Nicaud JM, Chardot T (2000) Purification and characterization of the recombinant form of Acyl CoA oxidase 3 from the yeast Yarrowia lipolytica. Arch Biochem Biophys 384:1–8

    Article  CAS  Google Scholar 

  • Luttik MA, Overkamp KM, Kotter P, de Vries S, van Dijken JP, Pronk JT (1998) The Saccharomyces cerevisiae NDE1 and NDE2 genes encode separate mitochondrial NADH dehydro genas es catalyzing the oxidation of cytosolic NADH. J Biol Chem 273:24529–24534

    Article  CAS  Google Scholar 

  • Madzak C, Treton B, Blanchin-Roland S (2000) Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. J Mol Microbiol Biotechnol 2:207–216

    CAS  Google Scholar 

  • Madzak M, Gaillardin C, Beckerich JM (2003) Hetrologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica. J. Biotech. In press

    Google Scholar 

  • Mamoun CB, Beckerich JM, Gaillardin C (1996) The TSR1 gene of Yarrowia lipolytica is involved in the signal recognition particle-dependent translocation pathway of secretory proteins. J Biol Chem 271:23895–23901

    Article  CAS  Google Scholar 

  • Mamoun CB, Beckerich JM, Gaillardin C (1997) Yarrowia lipolytica TSR1 gene product. A novel endoplasmic reticulum membrane component involved in the signal recognition particle-dependent translocation pathway. J Biol Chem 272:24594–24598

    Article  CAS  Google Scholar 

  • Mamoun CB, Beckerich JM, Gaillardin C, Kepes F (1999) Disruption of YHC8, a member of the TSR1 gene family, reveals its direct involvement in yeast protein translocation. J Biol Chem 274:11296–112302

    Article  CAS  Google Scholar 

  • Marres CA, de Vries S, Grivell LA (1991) Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH:ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. Eur J Biochem 195:857–62.

    Article  CAS  Google Scholar 

  • Mathiesen C, Hagerhall C (2002) Transmembrane topology of the NuoL, M and N subunits of NADH:quinone oxidoreductase and their homologues among membrane-bound hydrogenases and bona fide antiporters. Biochim Biophys Acta 1556:121–132

    Article  CAS  Google Scholar 

  • Matoba S, Fukayama J, Wing RA, Ogrydziak DM (1988) Intracellular precursors and secretion of alkaline extracellular protease of Yarrowia lipolytica. Mol Cell Biol 8:4904–4916

    CAS  Google Scholar 

  • Matsuoka M, Himeno T, Aiba S (1984) Characterization of Saccharomycopsis lipolytica mutants that express temperature-sensitive synthesis of isocitrate lyase. J Bacteriol 157:899–908

    CAS  Google Scholar 

  • Matsuoka M, Matsubara M, Inoue J, Kakehi M, Imanaka T (1994a) Organization and transcription of the mitochondrial ATP synthase genes in the yeast Yarrowia lipolytica. Curr Genet 26:382–389

    Article  CAS  Google Scholar 

  • Matsuoka M, Matsubara M, Kakehi M, Imanaka T (1994b) Homologous maturase-like proteins are encoded within the group I introns in different mitochondrial genes specifying Yarrowia lipolytica cytochrome c oxidase subunit 3 and Saccharomyces cerevisiae apocytochrome b. Curr Genet 26:377–381

    Article  CAS  Google Scholar 

  • Matsuoka M, Uchida K, Aiba S (1982) Cytoplasmic transfer of oligomycin resistance during protoplast fusion of Saccharomycopsis lipolytica. J Bacteriol 152:530–3.

    CAS  Google Scholar 

  • Matsuoka M, Ueda Y, Aiba S (1980) Role and control of isocitrate lyase in Candida lipolytica. J Bacteriol 144:692–697

    CAS  Google Scholar 

  • Mauersberger S (1991) Mutants of alkane oxidation in the yeasts Yarrowia lipolytica and Candida maltosa. In:Finogenova TV, Sharyshev AA (eds) Alkane metabolism and oversynthesis of metabolites by microorganisms. Centre for Biological Research USSR Academy of Sciences., Pushchino (Russia)

    Google Scholar 

  • Mauersberger S, Drechsler H, Oehme G, Muller H-G (1992) Substrate specificity and stereoselectivity of fatty alcohol oxidase from the yeast Candida maltosa. Appl. Microbiol. Biotechnol. 37:66–73

    Article  CAS  Google Scholar 

  • Mauersberger S, Matiashova RN (1980) [Cytochrome P-450 content in yeast cells during growth on hexadecane]. Mikrobiologiia 49:571–577

    CAS  Google Scholar 

  • Mauersberger S, Wang HJ, Gaillardin C, Barth G, Nicaud JM (2001) Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization. J Bacteriol 183:5102–5109

    Article  CAS  Google Scholar 

  • Medentsev AG, Akimenko VK (1999) Development and activation of cyanide-resistant respiration in the yeast Yarrowia lipolytica. Biochemistry (Mosc) 64:945–951

    CAS  Google Scholar 

  • Meuer J, Bartoschek S, Koch J, Kunkel A, Hedderich R (1999) Purification and catalytic properties of Ech hydrogenase from Methanosarcina barken. Eur J Biochem 265:325–335

    Article  CAS  Google Scholar 

  • Montet Y, Amara P, Volbeda A, Vernede X, Hatchikian EC, Field MJ, Frey M, Fontecilla-Camps JC (1997) Gas access to the active site of Ni-Fe hydrogenases probed by X-ray crystallography and molecular dynamics. Nat Struct Biol 4:523–526

    Article  CAS  Google Scholar 

  • Murphy GL, Perry JJ (1984) Assimilation of chlorinated alkanes by hydrocarbon-utilizing fungi. J Bacteriol 160:1171–4.

    CAS  Google Scholar 

  • Neuveglise C, Feldmann H, Bon E, Gaillardin C, Casaregola S (2002) Genomic evolution of the long terminal repeat retrotransposons in hernias cornycetous yeasts. Genome Res 12:930–943

    Article  CAS  Google Scholar 

  • Neuveglise C, Nicauda JM, Ross-Macdonald P, Gaillardin C (1998) A shuttle mutagenesis system for tagging genes in the yeast Yarrowia lipolytica. Gene 213:37–46

    Article  CAS  Google Scholar 

  • Nosek J, Fukuhara H (1994) NADH dehydrogenase subunit genes in the mitochondrial DNA of yeasts. J Bacteriol 176:5622–5630

    CAS  Google Scholar 

  • Nyns EJ, Auquiere JP, Chiang N, Wiaux AL (1967) Comparative growth of Candida lipolytica on glucose and n-hexadecane. Nature 215:177–178

    Article  CAS  Google Scholar 

  • Ogrydaak D, Bassel J, Mortimer R (1982) Development of the genetic map of the yeast Saccharomycopsis lipolytica. Mol Gen Genet 188:179–183

    Article  Google Scholar 

  • Ohnishi T (1998) Iron-sulfur clusters/semiquinones in complex I. Biochim Biophys Acta 1364:186–206

    Article  CAS  Google Scholar 

  • Overkamp KM, Bakker BM, Kotter P, van Tuijl A, de Vries S, van Dijken JP, Pronk JT (2000) In vivo analysis of the mechanisms for oxidation of cytosolic NADH by Saccharomyces cerevisiae mitochondria. J Bacteriol 182:2823–2830

    Article  CAS  Google Scholar 

  • Pagot Y, Le Clainche A, Nicaud JM, Wache Y, Belin JM (1998) Peroxisomal beta-oxidation activities and gamma-decalactone production by the yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 49:295–300

    Article  CAS  Google Scholar 

  • Papanikolaou S, Chevalot I, Komaitis M, Marc I, Aggelis G (2002a) Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures. Appl Microbiol Biotechnol 58:308–312

    Article  CAS  Google Scholar 

  • Papanikolaou S, Muniglia L, Chevalot I, Aggelis G, Marc I (2002b) Yarrowia lipolytica as a potential producer of citric acid from raw glycerol. J Appl Microbiol 92:737–744

    Article  CAS  Google Scholar 

  • Perez-Campo FM, Dominguez A (2001) Factors affecting the morphogenetic switch in Yarrowia lipolytica. Curr Microbiol 43:429–433

    Article  CAS  Google Scholar 

  • Perez-Campo FM, Nicaud JM, Gaillardin C, Dominguez A (1996) Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica. Yeast 12:1459–1469

    Article  CAS  Google Scholar 

  • Petit T, Gancedo C (1999) Molecular cloning and characterization of the gene HXK1 encoding the hexokinase from Yarrowia lipolytica. Yeast 15:1573–1584

    Article  CAS  Google Scholar 

  • Pignede G, Wang H, Fudalej F, Gaillardin C, Seman M, Nicaud JM (2000a) Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolytica. J Bacteriol 182:2802–28

    Article  CAS  Google Scholar 

  • Pignede G, Wang HJ, Fudalej F, Seman M, Gaillardin C, Nicaud JM (2000b) Autocloning and amplification of LIP2 in Yarrowia lipolytica. Appl Environ Microbiol 66:3283–3288

    Article  CAS  Google Scholar 

  • Purdue PE, Lazarow PB (2001) Peroxisome biogenesis. Annu Rev Cell Dev Biol 17:701–752

    Article  CAS  Google Scholar 

  • Queiroz-Claret C, Jolivet P, Chardot T, Bergeron E, Meunier JC (2000) Time-co-ordinated control of glycogen synthase, protein phosphatase 2A and protein kinase CK2 during culture growth in Yarrowia lipolytica in relation to glycogen metabolism. C R Acad Sci 111323:257–266

    Google Scholar 

  • Ramon AM, Gil R, Burgal M, Sentandreu R, Valentin E (1996) A novel cell wall protein specific to the mycelial form of Yarrowia lipolytica. Yeast 12:1535–1548

    Article  CAS  Google Scholar 

  • Ramon AM, Montero M, Sentandreu R, Valentin E (1999) Yarrowia lipolytica cell wall architecture: interaction of Ywp1, a mycelial protein, with other wall components and the effect of its depletion. Res Microbiol 150:95–103

    Article  CAS  Google Scholar 

  • Rasmussen T, Scheide D, Brors B, Kintscher L, Weiss H, Friedrich T (2001) Identification of two tetranuclear FeS clusters on the ferredoxin-type subunit of NADH:ubiquinone oxidoreductase (complex I). Biochemistry 40:6124–61

    Article  CAS  Google Scholar 

  • Richard M, Quijano RR, Bezzate S, Bordon-Pallier F, Gaillardin C (2001) Tagging morphogenetic genes by insertional mutagenesis in the yeast Yarrowia lipolytica. J Bacteriol 183:3098–3107

    Article  CAS  Google Scholar 

  • Robinson BH (1998) Human complex I deficiency: clinical spectrum and involvement of oxygen free radicals in the pathogenicity of the defect. Biochim Biophys Acta 1364:271–286

    Article  CAS  Google Scholar 

  • Romer MC, Hammer E, Cazau MC, Arambarri AM (2002) Isolation and characterization of biarylic structure-de grading yeasts: hydroxylation potential of dibenzofuran. Environ Pollut 118:379–382

    Article  Google Scholar 

  • Rycovska A, Szabo R, Tomaska L, Nosek J (2000) The respiratory complex I in yeast:isolation of a gene NUO51 coding for the nucleoti de-binding subunit of NADH:ubiquinone oxidoreductase from the obligately aerobic yeast Yarrowia lipolytica. Folia Microbiol 45:429–433

    Article  CAS  Google Scholar 

  • Sacksteder KA, Gould SJ (2000) The genetics of peroxisome biogenesis. Annu Rev Genet 34:623–652

    Article  CAS  Google Scholar 

  • Sanchez M, Beckerich JM, Gaillardin C, Dominguez A (1997) Isolation and cloning of the Yarrowia lipolytica SEC65 gene, a component of the yeast signal recognition particle displaying homology with the human SRP19 gene. Gene 203:75–84

    Article  CAS  Google Scholar 

  • Sazanov LA, Peak-Chew SY, Fearnley IM, Walker JE (2000) Resolution of the membrane domain of bovine complex I into subcomplexes:implications for the structural organization of the enzyme. Biochemistry 39:7229–7235

    Article  CAS  Google Scholar 

  • Schapira AH (1998) Human complex I defects in neuro de generative diseases. Biochim Biophys Acta 1364:261–270

    Article  CAS  Google Scholar 

  • Schmid-Berger N, Schmid B, Barth G(1994) Yltl, a highly repetitive retrotransposon in the genome of the dimorphic fungus Yarrowia lipolytica. JBacteriol 176:2477–2482

    CAS  Google Scholar 

  • Schon EA (2000) Mitochondrial genetics and disease. Trends Biochem Sci 25:555–560

    Article  CAS  Google Scholar 

  • Schuler F, Yano T, Di Bernardo S, Yagi T, Yankovskaya V, Singer TP, Casida JE (1999) NADH-quinone oxidoreductase:PSST subunit couples electron transfer from ironsulfur cluster N2 to quinone. Proc Natl Acad Sci USA 96:4149–4153

    Article  CAS  Google Scholar 

  • Schweizer E, Kottig H, Regler R, Rottner G (1988) Genetic control of Yarrowia lipolytica fatty acid synthetase biosynthesis and function. J Basic Microbiol 28:283–292

    Article  CAS  Google Scholar 

  • Smeitink J, van den Heuvel L (1999) Human mitochondrial complex I in health and disease. Am J Hum Genet 64:1505–1510

    Article  CAS  Google Scholar 

  • Souciet J, Aigle M, Artiguenave F, Blandin G, Bolotin-Fukuhara M, Bon E, Brottier P, Casaregola S, de Montigny J, Dujon B, Durrens P, Gaillardin C, Lepingle A, Llorente B, Malpertuy A, Neuveglise C, Ozier-Kalogeropoulos O, Potier S, Saurin W, Tekaia F, Toffano-Nioche C, Wesolowski-Louvel M, Wincker P, Weissenbach J (2000) Genomic exploration of the herniascornycetous yeasts: 1. A set of yeast species for molecular evolution studies. FEBS Lett 487:3–12

    Article  Google Scholar 

  • Subramani S (1993) Protein import into peroxisomes and biogenesis of the organelle. Annu Rev Cell Biol 9:445–478

    Article  CAS  Google Scholar 

  • Sumita T, Iida T, Yamagami S, Horiuchi H, Takagi M, Ohta A (2002) Y1ALK1 encoding the cytochrome P450ALK1 in Yarrowia lipolytica is transcriptionally induced by n-alkane through two distinct cis-elements on its promoter. Biochem Biophys Res Commun 294:1071–1078

    Article  CAS  Google Scholar 

  • Swennen D, Joyet P, Gaillardin C (1997) Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene. Curr Genet 31:128–132

    Article  CAS  Google Scholar 

  • Szabo R (1999) Dimorphism in Yarrowia lipolytica:filament fomiation is suppressed by nitrogen starvation and inhibition of respiration. Folia Microbiol. Praha 44:19–24

    Article  CAS  Google Scholar 

  • Szabo R (2001) Cla4 protein kinase is essential for filament formation and invasive growth of Yarrowia lipolytica. Mol Gen Genomics 265:172–179

    Article  CAS  Google Scholar 

  • Szilard RK, Titorenko VI, Veenhuis M, Rachubinski RA (1995) Pay32p of the yeast Yarrowia lipolytica is an intraperoxisomal component of the matrix protein translocation machinery. J Cell Biol 131:1453–1469

    Article  CAS  Google Scholar 

  • Tarn YY, Rachubinski RA (2002) Yarrowia lipolytica cells mutant for the PEX24 gene encoding a peroxisomal membrane peroxin mislocalize peroxisomal proteins and accumulate membrane structures containing both peroxisomal matrix and membrane proteins. Mol Biol Cell 13:2681–2691

    Article  CAS  Google Scholar 

  • Titorenko VI, Chan H, Rachubinski RA (2000a) Fusion of small peroxisomal vesicles in vitro reconstructs an early step in the in vivo multistep peroxisome assembly pathway of Yarrowia lipolytica. J Cell Biol 148:29–44

    Article  CAS  Google Scholar 

  • Titorenko VI, Nicaud JM, Wang H, Chan H, Rachubinski RA (2002) Acyl-CoA oxidase is imported as a heteropentameric, cofactor-containing complex into peroxisomes of Yarrowia lipolytica. J Cell Biol 156:481–94

    Article  CAS  Google Scholar 

  • Titorenko VI, Ogrydziak DM, Rachubinski RA (1997) Four distinct secretory pathways serve protein secretion, cell surface growth, and peroxisome biogenesis in the yeast Yarrowia lipolytica. Mol Cell Biol 17:5210–5226

    CAS  Google Scholar 

  • Titorenko VI, Rachubinski RA (1998a) The endoplasmic reticulum plays an essential role in peroxisome biogenesis. Trends Biochem Sci 23:231–233

    Article  CAS  Google Scholar 

  • Titorenko VI, Rachubinski RA (1998b) Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis. Mol Cell Biol 18:2789–2803

    CAS  Google Scholar 

  • Titorenko VI, Rachubinski RA (2000) Peroxisomal membrane fusion requires two AAA family ATPases, Pex1p and Pex6p. J Cell Biol 150:881–886

    Article  CAS  Google Scholar 

  • Titorenko VI, Rachubinski RA (2001a) Dynamics of peroxisome assembly and function. Trends Cell Biol 11:22–29

    Article  CAS  Google Scholar 

  • Titorenko VI, Rachubinski RA (2001b) The life cycle of the peroxisome. Nat Rev Mol Cell Biol 2:357–368

    Article  CAS  Google Scholar 

  • Titorenko VI, Smith JJ, Szilard RK, Rachubinski RA (1998) Pex20p of the yeast Yarrowia lipolytica is required for the oligomerization of thiolase in the cytosol and for its targeting to the peroxisome. J Cell Biol 142:403–420

    Article  CAS  Google Scholar 

  • Titorenko VI, Smith JJ, Szilard RK, Rachubinski RA (2000b) Peroxisome biogenesis in the yeast Yarrowia lipolytica. Cell Biochem Biophys 32:21–26

    Article  CAS  Google Scholar 

  • Torres-Guzman JC, Dominguez A (1997) HOY1, a homeo gene required for hyphal formation in Yarrowia lipolytica. Mol Cell Biol 17:6283–6293

    CAS  Google Scholar 

  • Torres-Guzman JC, Xoconostle-Cazares B, Guevara-Olvera L, Ortiz L, San-Bias G, Dominguez A, Ruiz-Herrera J (1996) Comparison of fungal ornithine decarboxylases. Curr Microbiol 33:390–392

    Article  CAS  Google Scholar 

  • Travers KJ, Patil CK, Wodicka L, Lockhart DJ, Weissman JS, Walter P (2000) Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation. Cell 101:249–258

    Article  CAS  Google Scholar 

  • Treton B, Blanchin-Roland S, Lambert M, Lepingle A, Gaillardin C (2000) Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus mduians genes palF and palH. Mol Gen Genet 263:505–513

    Article  CAS  Google Scholar 

  • Triepels EH, van den Heuvel LP, Loeffen JL, Buskens CA, Smeets RJ, Rubio Gozalbo ME, Budde SM, Mariman EC, Wijburg FA, Barth PG, Trijbels JM, Smeitink JA (1999) Leigh syndrome associated with a mutation in the NDUFS7 (PSST) nuclear encoded subunit of complex I. AnnNeurol 45:787–790

    CAS  Google Scholar 

  • Tzschoppe K, Augstein A, Bauer R, Kohlwein SD, Barth G (1999) trans-dominant mutations in the GPR1 gene cause high sensitivity to acetic acid and ethanol in the yeast Yarrowia lipolytica. Yeast 15:1645–1656

    Article  CAS  Google Scholar 

  • Uchiyama H, Ando M, Toyonaka Y, Tabuchi T (1982) Subcellular localization of the methylcitric-acid-cycle enzymes in propionate metabolism of Yarrowia lipolytica. Eur J Biochem 125:523–527

    Article  CAS  Google Scholar 

  • van den Bosch H, Schutgens RB, Wanders RJ, Tager JM (1992) Biochemistry of peroxisomes. Annu Rev Biochem 61:157–197

    Google Scholar 

  • van Heerikhuizen H, Ykema A, Klootwijk J, Gaillardin C, Ballas C, Fournier P (1985) Heterogeneity in the ribosomal RNA genes of the yeast Yarrowia lipolytica; cloning and analysis oftwo size classes ofrepeats. Gene 39:213–222

    Article  Google Scholar 

  • Verna J, Lodder A, Lee K, Vagts A, Ballester R (1997) A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 94:13804–13809

    Article  CAS  Google Scholar 

  • Vernis L, Abbas A, Chasles M, Gaillardin CM, Brun C, Huberman JA, Fournier P (1997) An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica. Mol Cell Biol 17:1995–2004

    CAS  Google Scholar 

  • Vernis L, Chasles M, Pasero P, Lepingle A, Gaillardin C, Fournier P (1999) Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica. Mol Biol Cell 10:757–769

    CAS  Google Scholar 

  • Vernis L, Poljak L, Chasles M, Uchida K, Casaregola S, Kas E, Matsuoka M, Gaillardin C, Fournier P (2001) Only centromeres can supply the partition system required for ARS function in the yeast Yarrowia lipolytica. J Mol Biol 305:203–217

    Article  CAS  Google Scholar 

  • Videira A (1998) Complex I from the fungus Neurospora crassa. Biochim Biophys Acta 1364:89–100

    Article  CAS  Google Scholar 

  • Wache Y, Aguedo M, Choquet A, Gatfield IL, Nicaud JM, Belin JM (2001) Role of beta-oxidation enzymes in gamma-decalactone production by the yeast Yarrowia lipolytica. Appl Environ Microbiol 67:5700–5704

    Article  CAS  Google Scholar 

  • Walker JE (1992) The NADH:ubiquinone oxidoreductase (complex I) of respiratory chains. Q Rev Biophys 25:253–324

    Article  CAS  Google Scholar 

  • Wanders RJ, Tager JM (1998) Lipid metabolism in peroxisomes in relation to human disease. Mol Aspects Med 19:69–154

    Article  CAS  Google Scholar 

  • Wanders RJ, Vreken P, Ferdinandusse S, Jansen GA, Waterham HR, van Roermund CW, Van Grunsven EG (2001) Peroxisomal fatty acid alpha-and beta-oxidation in humans: enzymology, peroxisomal metabolite transporters and peroxisomal diseases. Biochem Soc Trans 29:250–267

    Article  CAS  Google Scholar 

  • Wang H, Le Clainche A, Le Dall MT, Wache Y, Pagot Y, Belin JM, Gaillardin C, Nicaud JM (1998) Cloning and characterization of the peroxisomal acyl CoA oxidase ACO3 gene from the alkane-utilizing yeast Yarrowia lipolytica. Yeast 14:1373–1386

    Article  CAS  Google Scholar 

  • Wang H, Le Dall MT, Wache Y, Laroche C, Belin JM, Nicaud JM (1999a) Cloning, sequencing, and characterization of five genes coding for acyl-CoA oxidase isozymes in the yeast Yarrowia lipolytica. Cell Biochem Biophys 31:165–174

    Article  CAS  Google Scholar 

  • Wang HJ, Le Dall MT, Wach Y, Laroche C, Belin JM, Gaillardin C, Nicaud JM (1999b) Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica. JBacteriol 181:5140–5148

    CAS  Google Scholar 

  • Waterham HR, Titorenko VI, Swaving GJ, Harder W, Veenhuis M (1993) Peroxisomes in the methylotrophic yeast Hansenula polymorpha do not necessarily derive from pre-existing organelles. Embo J 12:4785–4794

    CAS  Google Scholar 

  • Wiederkehr A, Avaro S, Prescianotto-Baschong C, Haguenauer-Tsapis R, Riezman H (2000) The F-box protein Rcylp is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae. J Cell Biol 149:397–410.

    Article  CAS  Google Scholar 

  • Wikstrom M (1984) Pumping of protons from the mitochondrial matrix by cytochrome oxidase. Nature 308:558–560

    Article  CAS  Google Scholar 

  • Yagi T, Yano T, Di Bernardo S, Matsuno-Yagi A (1998) Procaryotic complex I (NDH-1), an overview. Biochim Biophys Acta 1364:125–133

    Article  CAS  Google Scholar 

  • Yamagami S, Iida T, Nagata Y, Ohta A, Takagi M (2001) Isolation and characterization of acetoacetyl-CoA thiolase gene essential for n-decane assimilation in yeast Yarrowia lipolytica. Biochem Biophys Res Commun 282:832–838

    Article  CAS  Google Scholar 

  • Yaver DS, Matoba S, Ogrydziak DM (1992) A mutation in the signal recognition particle 7S RNA of the yeast Yarrowia lipolytica preferentially affects synthesis of the alkaline extracellular protease:in vivo evidence for translataonal arrest. J Cell Biol 116:605–616

    Article  CAS  Google Scholar 

  • Young LY, Lorenz MC, Heitman J (2000) A STE12 homolog is required for mating but dispensable for filamentation in Candida lusitamae. Genetics 155:17–29

    CAS  Google Scholar 

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  1. Gerold Barth
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  2. Jean-Marie Beckerich
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  1. Beyerinck Laboratory DSM Life Sciences, Bakery Ingredients Technology, PO Box 1, 2600 MA, Delft, The Netherlands

    Johannes H. de Winde

  2. Kluyver Laboratory for Biotechnology, Technical University of Delft, Julianalaan 67, 2628 BC, Delft, The Netherlands

    Johannes H. de Winde

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Barth, G. et al. (2003). Functional genetics of Yarrowia lipolytica . In: de Winde, J.H. (eds) Functional Genetics of Industrial Yeasts. Topics in Current Genetics, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-37003-X_8

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