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Yeast functional genomics and metabolic engineering: past, present and future

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Book cover Functional Genetics of Industrial Yeasts

Part of the book series: Topics in Current Genetics ((TCG,volume 2))

Abstract

In recent years, metabolic engineering has been applied successfully for improvement of various fermentation processes. However, even single genetic changes usually result in multigene responses, which make it difficult to predict and understand the effects of introduced genetic changes. This is a direct consequence of complex regulatory systems and of redundancy in the control of pathway fluxes. Challenges in metabolic engineering therefore, involve multiple genetic changes and often engineering of complete regulatory pathways. For this reason metabolic engineering involves studies on cellular physiology and reconstruction of regulatory networks. Analytical techniques employed in functional genomics enable a global, whole-cell view and have thus become invaluable in metabolic engineering strategies. These techniques allow understanding of the complexity of cellular metabolism and insight into the cellular effects of genetic modifications introduced.

Here we review functional genomics and metabolic engineering in yeast, discuss how these research areas interact, and present ideas and examples of the use of functional genomics tools in the process of metabolic engineering.

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Authors
  1. Christoffer Bro
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  2. Birgitte Regenberg
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  3. Jens Nielsen
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Editors and Affiliations

  1. Beyerinck Laboratory DSM Life Sciences, Bakery Ingredients Technology, PO Box 1, 2600 MA, Delft, The Netherlands

    Johannes H. de Winde

  2. Kluyver Laboratory for Biotechnology, Technical University of Delft, Julianalaan 67, 2628 BC, Delft, The Netherlands

    Johannes H. de Winde

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© 2003 Springer-Verlag Berlin Heidelberg

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Bro, C., Regenberg, B., Nielsen, J. (2003). Yeast functional genomics and metabolic engineering: past, present and future. In: de Winde, J.H. (eds) Functional Genetics of Industrial Yeasts. Topics in Current Genetics, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-37003-X_11

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  • DOI: https://doi.org/10.1007/3-540-37003-X_11

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  • Print ISBN: 978-3-540-02489-7

  • Online ISBN: 978-3-540-37003-1

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