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Mass Spectrometry — a Key Technology in Proteom Research

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Proteomics of Microorganisms

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 83))

Abstract

The rapid developments in the field of mass spectrometry have transformed it into a key technology in proteome research.

Increased sensitivity in mass spectrometry, as a result of more efficient ionisation techniques and better detectors, has allowed the stepwise reduction of protein quantity for analysis. Protein spots of 2D-PAGE separated samples are now quantitatively sufficient for an unequivocal identification of a protein by mass spectrometry. In addition to protein identification a closer look at posttranslational modifications is now also possible. It is speculated that modifications like phosphorylation or glycosylation exist on every second protein and that they are important for the protein function.

This review highlights the different mass spectrometric methods and gives a brief overview of strategies and methods used to identify modifications.

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Author information

Authors and Affiliations

  1. Rudolf-Virchow-Zentrum, DFG Forschungszentrum für Experimentelle Biomedizin, Versbacher Strasse 9, Raum 411, 97078, Würzburg, Germany

    Albert Sickmann

  2. PROT@GEN AG, Emil-Figge-Strasse 76A, 44227, Dortmund, Germany

    Marcus Mreyen

  3. Medical Proteom Center, Ruhr University of Bochum, ZKF E. 143, 44780, Bochum, Germany

    Helmut E. Meyer

Authors
  1. Albert Sickmann
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  2. Marcus Mreyen
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  3. Helmut E. Meyer
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Editors and Affiliations

  1. Greifswald Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität, Friedrich-Ludwig-Jahn-Straße 15, 17487, Greifswald, Germany

    M. Hecker

  2. Fundamenta Capital AG, Bergische Landstrasse 67, 51375, Leverkusen, Germany

    Stefan Müllner (Senior Vice President Life Sciences) (Senior Vice President Life Sciences)

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© 2003 Springer-Verlag Berlin Heidelberg

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Sickmann, A., Mreyen, M., Meyer, H.E. (2003). Mass Spectrometry — a Key Technology in Proteom Research. In: Hecker, M., et al. Proteomics of Microorganisms. Advances in Biochemical Engineering/Biotechnology, vol 83. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-36459-5_6

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  • DOI: https://doi.org/10.1007/3-540-36459-5_6

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-00546-9

  • Online ISBN: 978-3-540-36459-7

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