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Oxidative Stress in Sepsis: Implications on Liver Protein Patterns and Analysis via Modified Proteomics Technology

  • Conference paper
Yearbook of Intensive Care and Emergency Medicine

Part of the book series: Yearbook of Intensive Care and Emergency Medicine ((YEARBOOK,volume 2006))

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Conclusion

Taken together, proteins of the antioxidant defense system can be damaged by oxidative stress, and, in fact, there is evidence that they are even specifically susceptible. The oxidative loss of protein moiety is partially compensated by de novo synthesis. This compensatory mechanism complicates any attempt to relate mRNA profiles assessed by cDNA technology or protein expression profiles assessed by 2D-gel electrophoresis to the functionally active protein content. The dynamic cellular response with sepsis can only be revealed by disentangling the enormously complex response at the protein level. The only method able to deliver appropriate information is a proteomic platform based on differential and quantitative approaches, which is extended by synthesis or turnover measurements.

Our ultimate aim is to use this dynamic approach:

  • to understand the complex interaction between the various elements of the defense system;

  • to define a set of measurements necessary to characterize the various conditions of the system; and

  • to develop a tool box to evaluate the efficacy of therapeutic measures intended to support the defense system.

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Authors and Affiliations

  1. Pathophysiology Section, University Anesthesiology Clinic, Parkstraße 11, 89073, Ulm, Germany

    J. A. Vogt, P. Radermacher & E. Barth

Authors
  1. J. A. Vogt
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  2. P. Radermacher
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  3. E. Barth
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Editors and Affiliations

  1. Department of Intensive Care, Erasme Hospital, Free University of Brussels, Route de Lennik 808, B-1070, Brussels, Belgium

    Jean-Louis Vincent (Head) (Head)

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© 2006 Springer-Verlag Berlin Heidelberg

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Vogt, J.A., Radermacher, P., Barth, E. (2006). Oxidative Stress in Sepsis: Implications on Liver Protein Patterns and Analysis via Modified Proteomics Technology. In: Vincent, JL. (eds) Yearbook of Intensive Care and Emergency Medicine. Yearbook of Intensive Care and Emergency Medicine, vol 2006. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-33396-7_3

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  • DOI: https://doi.org/10.1007/3-540-33396-7_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-30155-4

  • Online ISBN: 978-3-540-33396-8

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