4 Concluding Remarks
Single-cell analysis of BY-2 that can solely be performed with microinjection hasbeendemonstrated. This shows thatmicroinjection is feasible asapractical methodeven for small cellsnogreater than 50µm in diameter. It is expectedthat the spatio-temporal precision of microinjection will become more advanced owing to progress in nanotechnology. Then microinjection would be referred to as nanoinjection. Such an advancement ofmicroinjectionwill surely support the functional analysis of many proteins of gene expression products as well as other associated bioactive compounds.
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Matsuoka, H., Yamada, Y., Matsuoka, K., Saito, M. (2006). High Throughput Microinjection Technology for the Single-Cell Analysis of BY-2 in Vivo. In: Nagata, T., Matsuoka, K., Inzé, D. (eds) Tobacco BY-2 Cells: From Cellular Dynamics to Omics. Biotechnology in Agriculture and Forestry, vol 58. Springer, Berlin, Heidelberg . https://doi.org/10.1007/3-540-32674-X_22
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