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Experimental In Vitro Exposure Methods for Studying the Effects of Inhalable Compounds

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Cigarette Smoke and Oxidative Stress

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References

  • Aerts C, Wallaert B, Voisin C (1992) In vitro hyperoxia on alveolar type II pneumocytes: inhibition of glutathione synthesis increases hyperoxic cell injury. Exp Lung Res 18: 845–861

    PubMed  CAS  Google Scholar 

  • Adler KB, Schwarz JE, Whitcutt MJ, Wu R (1987) A new chamber system for maintaining differentiated guinea pig respiratory epithelial cells between air and liquid phases. Biotech 5:462–465

    Google Scholar 

  • Aufderheide M, Mohr U (1999) CULTEX—a new system and technique for the cultivation and exposure of cells at the air/liquid interface. Exp Toxic Pathol 51:489–490

    CAS  Google Scholar 

  • Aufderheide M, Mohr U (2000) CULTEX—an alternative technique for the cultivation and exposure of cells of the respiratory tract to airborne pollutants at the air/liquid interface. Exp Toxic Pathol 52:265–270

    CAS  Google Scholar 

  • Aufderheide M, Ritter D, Knebel JW, Scherer G (2001) A method for in vitro analysis of the biological activity of complex mixtures such as sidestream cigarette smoke. Exp Toxic Pathol 53141–152

    Google Scholar 

  • Baker FD, Tumasonis CF (1971) Modified roller drum apparatus for analysing effects of pollutant gases on tissue culture systems. Atmos Environ 5891–893

    Google Scholar 

  • Boland S, Bonvallot V, Fournier T, Baeza-Squiban A, Aubier M, Marano F (2000) Mechanisms of GM-CSF increase by diesel exhaust particles in human airway epithelial cells. Am J Physiol 278:L25–L32

    CAS  Google Scholar 

  • Bolton DC, Tarkington BK, Zee YC, Osebold JW (1982) An in vitro system for studying the effects of ozone on mammalian cell cultures and viruses. Environ Res 27:466–475

    Article  PubMed  CAS  Google Scholar 

  • Bombick DW, Bombick BR, Ayres PH, Putnam K, Avalos J, Borgerding MF, Doolittle DJ (1997) Evaluation of the genotoxic and cytotoxic potential of mainstream whole smoke and smoke condensate from a cigarette containing a novel carbon filter. Fundam Appl Toxicol 39:11–17

    Article  PubMed  CAS  Google Scholar 

  • Cardile V, Jiang X, Russo A, Casella F, Renis M, Bindoni M (1995) Effects of ozone on some biological activities of cells in vitro. Cell Biol Toxicol 11:11–21

    PubMed  CAS  Google Scholar 

  • Comhair SA, Erzurum SC (2002) Antioxidant responses to oxidant-mediated lung diseases. Am J Physiol 283:L246–L255

    CAS  Google Scholar 

  • Dickinson DA, Moellering DR, Iles KE, Patel RP, Levonen AL, Wigley A, Darley-Usmar VM, Forman H (2003) Cytoprotection against oxidative stress and the regulation of glutathione synthesis. Biol Chem 384:527–537

    Article  PubMed  CAS  Google Scholar 

  • Don Porto Carero A, Hoet PH, Verschaeve L, Schoeters G, Nemery B (2001) Genotoxic effects of carbon black particles, diesel exhaust particles, and urban air particulates and their extracts on a human alveolar epithelial cell line (A549) and a human monocytic cell line (THP-1). Environ Mol Mutagen 37:155–163

    Article  PubMed  CAS  Google Scholar 

  • Fischer GL, Placke ME (1987) In vitro models of lung toxicity. Toxicology 47:71–93

    Article  Google Scholar 

  • Friedman M, Madden MC, Saunders DS, Gammon K, White GC II, Kwock L (1985) Ozone inhibits prostacyclin synthesis in pulmonary epithelium. Prostaglandins 30:1069–1085

    Article  PubMed  CAS  Google Scholar 

  • Friedman M, Madden MC, Samet JM, Koren HS (1992) Effects of ozone exposure on lipid metabolism in human alveolar macrophages. Environ Health Perspect. 97:95–101

    Article  PubMed  CAS  Google Scholar 

  • Guerrero RR, Rounds DE, Booher J, Olson RS, Hackney JD (1979a) Ozone sensitivity in aging WI-38 cells based on acid phosphatase content. Arch Environ Health 34:407–412

    PubMed  CAS  Google Scholar 

  • Guerrero RR, Rounds DE, Booher J, Olson RS, Hackney JD (1979b) Mutagenic effects of ozone on human cells exposed in vivo and in vitro based on sister chromatid exchange analysis. Environ Res 18:336–346

    Article  PubMed  CAS  Google Scholar 

  • Hagar WL, Sweet WE, Sweet F (1981) An in vitro system for assessing lung cell response to ozone. J Air Pollut Control Assoc 31:993–995

    CAS  Google Scholar 

  • Hoffmann D, Wynder EL (1986) Chemical constituents and bioactivity of tobacco smoke. IARC Sci Pub 74:145–165

    Google Scholar 

  • Hukkanen J, Lassila A, Paivarinta K, Valanne S, Sarpo S, Hakkola J, Pelkonen O, Raunio H (2000) Induction and regulation of xenobiotic-metabolizing cytochrome P450s in the human A549 lung adenocarcinoma cell line. Am J Respir Cell Mol Biol 22:360–366

    PubMed  CAS  Google Scholar 

  • Iwanari M, Nakajima M, Kizu R, Hayakawa K, Yokoi T (2002) Induction of CYP1A1, CYP1A2, and CYP1B1 mRNAs by nitropolycyclic aromatic hydrocarbons in various human tissue-derived cells: chemical-, cytochrome P450 isoform-, and cell-specific differences. Arch Toxicol 76:287–298

    Article  PubMed  CAS  Google Scholar 

  • Janic B, Umstead TM, Phelps DS, Floros J (2003) An in vitro model system for the study of the effects of ozone and other gaseous agents on phagocytic cells. J Immunol Methods 272:125–134

    Article  PubMed  CAS  Google Scholar 

  • Leikauf G, Driscoll K (1993) Cellular approaches in respiratory tract toxicology. In Gardner DE et al. (eds) Toxicology of the lung, 2nd ed. Raven, New York, pp 335–370

    Google Scholar 

  • Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G (1976) A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer 17:62–70

    Article  PubMed  CAS  Google Scholar 

  • Le Prieur E, Vaz E, Bion A, Dionnet F, Morinn J-P (2000) Toxicity of diesel engine exhaust in an in vitro model of lung slices in biphasic organotypic culture: induction of a proinflammatory and apoptotic response. Arch Toxicol 74:460–466

    Article  PubMed  Google Scholar 

  • Li XY, Donaldson K, Rahman I, MacNee W (1994) An investigation of the role of glutathione in increased epithelial permeability induced by cigarette smoke in vivo and in vitro. Am J Respir Crit Care Med 149:1518–1525

    PubMed  CAS  Google Scholar 

  • Madden MC, Eling TE, Dailey LA, Friedman M (1991) The effect of ozone exposure on rat alveolar macrophage arachidonic acid metabolism. Exp Lung Res 17:47–63

    PubMed  CAS  Google Scholar 

  • Mendelson CR, Boggaram V (1991) Hormonal control of the surfactant system in fetal lung. Annu Rev Physiol 53:415–440

    Article  PubMed  CAS  Google Scholar 

  • Morin J-P, Fouquet F, Monteil C, Le Prieur E, Dionnet F (1999) Development of a new in vitro system for continuous in vitro exposure of lung tissue to complex atmospheres: Application to diesel exhaust toxicology. Cell Biol Toxicol 15:143–152

    Article  PubMed  CAS  Google Scholar 

  • Muckter H, Zwing M, Bader S, Marx T, Doklea E, Liebl B, Fichtl B, Georgieff M (1998) A novel apparatus for the exposure of cultured cells to volatile agents. J Pharmacol Toxicol Methods 40:63–69

    Article  PubMed  CAS  Google Scholar 

  • Nardone LL, Andrews SB (1979) Cell line A549 as a model of type II pneumocytes. Phospholipid biosynthesis from native and organometallic precursors. Biochim Biophys Acta 573:276–295

    PubMed  CAS  Google Scholar 

  • Nikula KJ, Wilson DW, Dungworth DL, Plopper CG (1988) In vitro evidence of cellular adaptation to ozone toxicity in the rat trachea. Toxicol Appl Pharmacol 93:394–402

    Article  PubMed  CAS  Google Scholar 

  • Nikula KJ, Wilson DW (1990) Response of rat tracheal epithelium to ozone and oxygen exposure in vitro. Fund Appl Toxicol 15:121–131

    Article  CAS  Google Scholar 

  • Pace DM, Landolt PA, Aftonomos BT (1969) Effects of ozone on cells in vitro. Arch Environ Health 18:165–170

    PubMed  CAS  Google Scholar 

  • Pace DM, Thompson JR, Aftonomos BT (1961) The effects of NO2 and salts of NO2 upon established cell lines. Can J Biochem Physiol 39:1247–1255

    PubMed  CAS  Google Scholar 

  • Park EM, Park YM, Gwak YS (1998) Oxidative damage in tissues of rats exposed to cigarette smoke. Free Radical Biol Med 25:79–86

    Article  CAS  Google Scholar 

  • Piperi C, Pouli AE, Katerelos NA, Hatzinikolaou DG, Stavridou A, Psallidopoulus AC (2003) Study of the mechanisms of cigarette smoke gas phase cytotoxicity. Anticancer Res 23:2185–2190

    PubMed  CAS  Google Scholar 

  • Pouli AE, Hatzinikolaou DG, Piperi C, Stavridou A, Psallidopoulos MC, Stavrides (2003) The cytotoxic effects of volatile organic compounds of the gas phase of cigarette smoke on lung epithelial cells. Free Radical Biol Med 34:345–355

    Article  CAS  Google Scholar 

  • Pryor WA (1992) Biological effects of cigarette smoke, wood smoke, and the smoke from plastics: the use of electron spin resonance. Free Radical Biol Med 13:659–676

    Article  CAS  Google Scholar 

  • Pryor WA (1997) Cigarette smoke radicals and the role of free radicals in chemical carcinogenicity. Environ Health Perspect 105:S875–S882

    Article  Google Scholar 

  • Rahman I, Li XY, Donaldson K, Harrison DJ, MacNee W (1995) Glutathione homeostasis in alveolar epithelial cells in vitro and lung in vivo under oxidative stress. Am J Physiol 269(3 Pt 1): L285–L292

    PubMed  CAS  Google Scholar 

  • Rahman I, Smith CA, Lawson MF, Harrison DJ, MacNee W (1996) Induction of gamma-glutamylcysteine synthetase by cigarette smoke is associated with AP-1 in human alveolar epithelial cells. FEBS Lett 396:21–25

    Article  PubMed  CAS  Google Scholar 

  • Rahman I, MacNee W (1999) Lung glutathione and oxidative stress: implications in cigarette smoke-induced airway disease. Am J Physiol 277(6 Pt 1):L1067–L1088

    PubMed  CAS  Google Scholar 

  • Rasmussen RE, Crocker TT (1982) Lung cells grown on cellulose membrane filters as an in vitro model of the respiratory epithelium. In: Tice RR, Costa DL, Schaich KM (eds) Genotoxic effects of airborne agents. Plenum, New York, pp 105–120

    Google Scholar 

  • Rasmussen RE (1984) In vitro system for exposure of lung cells to NO2 and O3. J Toxicol Environ Health 13:397–411

    PubMed  CAS  Google Scholar 

  • Ritter D, Knebel JW, Aufderheide M (2003) Exposure of human lung cells to inhalable substances: a novel test strategy involving clean air exposure periods using whole diluted cigarette mainstream smoke. Inhalation Toxicol 15:67–84

    Article  CAS  Google Scholar 

  • Ritter D, Knebel JW, Aufderheide M (2004) Comparative assessment of toxicities of mainstream smoke from commercial cigarettes. Inhal Toxicol 16:691–700

    Article  PubMed  CAS  Google Scholar 

  • Rusznak C, Devalia JL, Sapsford RJ, Davies RJ (1996) Ozone-induced mediator release from human bronchial epithelial cells in vitro and the influence of nedocromil sodium. Eur Respir J 9:2298–2305

    Article  PubMed  CAS  Google Scholar 

  • Samuelson GS, Rasmussen RE, Nair BK, Crocker TT (1978) Novel culture and exposure system for measurement of effects of airborne pollutants on mammalian cells. Environ Sci Technol 12:426–430

    Article  Google Scholar 

  • Smith BT (1977) Cell line A549: a model system for the study of alveolar type II cell function. Am Rev Respir Dis 115:285–293

    PubMed  CAS  Google Scholar 

  • Smith FB, Kikkawa Y, Diglio CA, Dalen RC (1982) Increased saturated phospholipid in cultured cells grown with linoleic acid. In Vitro 18:331–338

    PubMed  CAS  Google Scholar 

  • Sun W, Wu R, Last JA (1995) Effects of exposure to environmental tobacco smoke on a human tracheobronchial epithelial cell line. Toxicol 100:163–174

    Article  CAS  Google Scholar 

  • Tarkington BK, Wu R, Sun W-M, Nikula KJ, Wilson DW, Last JA (1994) In vitro exposure of tracheobronchial epithelial cells and of tracheal explants to ozone. Toxicol 88:51–68

    Article  CAS  Google Scholar 

  • Teague SV, Pinkerton KE, Goldsmith M, Gebremichael A, Chang S, Jenkins RA, Moneylun JH (1994) A sidestream cigarette smoke generation and exposure system for environmental tobacco smoke studies. Inhal Toxicol 6:79–93

    CAS  Google Scholar 

  • Umstead TM, Wang G, Phelps DS, Floros J, Tarkington BK (2002) In vitro exposure of proteins to ozone. Toxicol Mech Methods 12:1–16

    Article  CAS  Google Scholar 

  • Uejima Y, Fukuchi Y, Nagase T, Matsuse T, Yamaoka M, Tabata R, Orimo H (1990) Influence of inhaled tobacco smoke on the senescence accelerated mouse (SAM). Eur Respir J 3:1029–1036

    PubMed  CAS  Google Scholar 

  • Urani C, Doldi M, Crippa S, Camatini M (1998) Human-derived cell lines to study xenobiotic metabolism. Chemosphere 37(14–15):2785–2795

    Article  PubMed  CAS  Google Scholar 

  • Valentine R (1985) An in vitro system for exposure of lung cells to gases: effects of ozone on rat macrophages. J Toxicol Environ Health 16:115–126

    Article  PubMed  CAS  Google Scholar 

  • Van der Zee J, Tijssen Christianse K, Dubbelman TM, Van Steveninck J (1987) The influence of ozone on human red blood cells. Comparison with other mechanisms of oxidative stress. Biochim Biophys Acta 924:111–118

    PubMed  Google Scholar 

  • Voisin C, Aerts C, Houdret JL, Tonnel AB (1974) Effect of NO2 on alveolar macrophages. In: Voisin C (ed) Réactions bronchopulmonaires aux pollutants atmosphes [in French]. INSERM, Paris, pp 273–288

    Google Scholar 

  • Voisin C, Aerts C, Jakubsczak E, Houdret JL, Tonnel AB (1977a) Effets du bioxyde d’azote sur les macrophages alvéolaires en survie en phase gazeuse: un nouveau modèle expérimental pour l’étude in vitro de la cytotoxicité des gaz nocifs [in French]. Bull Eur Physiopath Respir 13:137–144

    CAS  Google Scholar 

  • Voisin C, Aerts C, Jakubsczak E, Tonnel AB (1977b) La culture cellulaire en phase gazeuse. Un nouveau modèle expérimental d’étude in vitro des activités des macrophages [in French]. Bull Eur Physiopath Respir 13:69–82

    CAS  Google Scholar 

  • Voisin C, Aerts C, Tonnel AB (1975) Mise en survie en phase gazeuse et reconstitution in vitro du microenvironnement naturel des macrophages alvéolaires [in French]. Pathol Biol 23:453–459

    PubMed  CAS  Google Scholar 

  • Wallaert B, Coursier JM, Gosset Ph, Saison JY, Tonnel AB (1998) Effects of urban air solution on cytokines secretion by alveolar macrophages in vitro. Eur Resp J 12:260S

    Google Scholar 

  • Wallaert B, Fahy O, Tsicopoulos A, Gosset Ph, Saison JY, Tonnel AB (2000) Experimental systems for mechanistic studies of toxicant induced lung inflammation. Toxicol Letters 112–113:157–163

    Article  Google Scholar 

  • Wallaert B, Gosset Ph, Boitelle A, Tonnel AB (1996) In vitro assessment of environmental toxicology using alveolar cells as a target. Cell Biol Toxicol 12:251–256

    Article  PubMed  CAS  Google Scholar 

  • Wenzel DG, Wrobel WJ, Kotek JJ, Morgan DL (1979) An ozone-generating system and chamber for testing injury to cultured cells. Arch Environ Health 34:454–460

    PubMed  CAS  Google Scholar 

  • Wenzel DG, Morgan DL (1982) Role of in vitro factors in ozone toxicity for cultured rat lung fibroblasts. Drug Chem Toxicol 5:201–217

    PubMed  CAS  Google Scholar 

  • Whitcutt MJ, Adler KB, Wu R (1988) A biphasic chamber system for maintaining polarity of differentiation of cultured respiratory tract epithelial cells. In vitro Cell Develop Biol 24:420–428

    Article  CAS  Google Scholar 

  • Young PP, Mendelson CR (1997) A GT box element is essential for basal and cyclic adenosine 3′,5′-monophosphate regulation of the human surfactant protein A2 gene in alveolar type II cells: evidence for the binding of lung nuclear factors distinct from Sp1. Mol Endocrinol 11:1082–1093

    Article  PubMed  CAS  Google Scholar 

  • Zamora PO, Gregory RE, Li AP, Brooks AL (1986) An in vitro model for the exposure of lung alveolar epithelial cells to toxic gases. J Environ Pathol Toxicol Oncol 7:159–168

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Fraunhofer Institute of Toxicology and Aerosol Research Drug Research and Clinical Inhalation, Nikolai-Fuchs-Strasse 1, 30625, Hannover, Germany

    Michaela Aufderheide

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  1. Michaela Aufderheide
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  1. Pharmacology Group, University of London King’s College, Manresa Road, London, SW3 6LX, UK

    Barry B. Halliwell

  2. Dept. of Clinical Pharmalogy, Rigshospitalet, University Hospital Copenhagen, Tagensvej 20, 2200, Copenhagen N, Denmark

    Henrik E. Poulsen

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Aufderheide, M. (2006). Experimental In Vitro Exposure Methods for Studying the Effects of Inhalable Compounds. In: Halliwell, B.B., Poulsen, H.E. (eds) Cigarette Smoke and Oxidative Stress. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-32232-9_10

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  • DOI: https://doi.org/10.1007/3-540-32232-9_10

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