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Biological Nitrogen Fixation: A Key Process for the Response of Grassland Ecosystems to Elevated Atmospheric [CO2]

  • Ueli A. Hartwig
  • Michael J. Sadowsky
Part of the Ecological Studies book series (ECOLSTUD, volume 187)

18.7 Conclusion

Data from our 10 years of studies done at the Swiss FACE site, along with numerous process studies done by ourselves and by others, clarifies the role that biological (symbiotic) N2 fixation plays in a CO2-rich world.
  • Under e[CO2], symbiotic N2 fixation increases as a result of increased plant growth (N demand) and not due to direct CO2 stimulation leading to greater photosynthate availability.

  • Under fertile soil conditions, e[CO2] apparently caused changes in soil processes and nitrogen status; and, as a result, increases in total symbiotic N2 fixation (N sink-driven) and changes in the population structure of N2-fixing soil micro-organisms were detected.

  • Under fertile soil conditions and ample water availability, e[CO2] apparently caused a pronounced N limitation in the initial periods of CO2 enhancement. However, within a few years, a new N balance was apparently reached (progressive N saturation); but only under conditions of high-N input and not under a low-N input. This was accompanied by a readjustment of symbiotic N2 fixation capacity in legumes and by further shifts in the population of N2-fixing soil micro-organisms to a structure seen previous to CO2 enrichment.

  • The integrated nature and interdependence of photosynthesis and biological (symbiotic) N2 fixation was confirmed from the Swiss FACE experiment.

Keywords

Plant Soil White Clover Arbuscular Mycorrhiza Perennial Ryegrass Grassland Ecosystem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ainsworth EA, Rogers A, Blum H, Nösberger J, Long SP (2003) Variation in acclimation of photosynthesis in Trifolium repens after eight years of exposure to free air CO2 enrichment (FACE). J Exp Bot 54:2769–2774PubMedCrossRefGoogle Scholar
  2. Almeida JFP, Lüscher A, Frehner M, Oberson A, Nösberger J (1999) Partitioning of P and the activity of root acid phosphatase in white clover (Trifolium repens L) are modified by increased atmospheric CO2 and P fertilisation. Plant Soil 210:159–166CrossRefGoogle Scholar
  3. Almeida JPF, Hartwig UA, Frehner M, Nösberger J, Lüscher A (2000) Evidence that P deficiency induces N feedback regulation of symbiotic N2 fixation in white clover (Trifolium repens L). J Exp Bot 51:1289–1297PubMedCrossRefGoogle Scholar
  4. Arnone JA III (1997) Indices of plant N availability in an alpine grassland under elevated CO2. Plant Soil 190:61–66CrossRefGoogle Scholar
  5. Arnone JA III (1999) Symbiotic N2 fixation in a high Alpine grassland: effects of four growing seasons of elevated CO2. Funct Ecol 13:383–387CrossRefGoogle Scholar
  6. Baggs EM, Richter M, Cadisch G, Hartwig UA (2003a) Denitrification in grass swards is increased under elevated atmospheric CO2. Soil Biol Biochem 35:729–732CrossRefGoogle Scholar
  7. Baggs EM, Richter M, Hartwig UA, Cadisch G (2003b) Nitrous oxide emissions from grass swards during eight years of elevated atmospheric pCO2 (Swiss FACE). Global Change Biol 9:1214–1222CrossRefGoogle Scholar
  8. Barnard R, Barthes L, Le Roux X, Harmens H, Raschi A, Soussana J-F, Winkler B, Leadley PW (2004) Atmospheric CO2 elevation has little effect on nitrifying and denitrifying activity in four European grasslands. Global Change Biol 10:488–497CrossRefGoogle Scholar
  9. Cadisch G, Sylvester-Bradley R, Boller BC, Nösberger J (1993) Effects of phosphorus and potassium on N2 fixation (15N-dilution) on field-grown Centrosema acutifolium and C. macrocarpum. Field Crop Res 31:329–340CrossRefGoogle Scholar
  10. Casella E, Soussana J-F, Loiseau P (1996) Long-term effects of CO2 enrichment and temperature increase on temperate grass sward. Plant Soil 182:83–99CrossRefGoogle Scholar
  11. Daepp M, Suter D, Almeida JPF, Isopp H, Hartwig UA, Frehner M, Blum H, Nösberger J, Lüscher A (2000) Yield response of Lolium perenne swards to free air CO2 enrichment increased over six years in a high-N input system. Global Change Biol 6:805–816CrossRefGoogle Scholar
  12. Daepp M, Nösberger J, Lüscher A (2001) Nitrogen fertilisation and development stage affect the response of yield, biomass partitioning and morphology of Lolium perenne L swards to elevated pCO2. New Phytol 150:347–358CrossRefGoogle Scholar
  13. Deiglmayr K, Philippot L, Hartwig UA, Kandeler E (2004) Structure and activity of the nitrate reducing community in the rhizosphere of Lolium perenne and Trifolium repens under long-term elevated atmospheric pCO2. FEMS Microbiol Ecol 49:445–454CrossRefPubMedGoogle Scholar
  14. Diaz S, Grime JP, Harris J, McPherson E (1993) Evidence of a feedback mechanism limiting plant response to elevated carbon dioxide. Nature 364:616–617CrossRefGoogle Scholar
  15. Dong Z, Layzell DB (2002) H2 oxidation, O2 uptake and CO2 fixation in hydrogen treated soils. Plant Soil 229:1–12CrossRefGoogle Scholar
  16. Feng Z, Cyckmans J, Flessa H (2004) Effects of elevated carbon dioxide concentration on growth and N2 fixation of young Rhobinia pseudoacacia. Tree Physiol 24:323–330PubMedGoogle Scholar
  17. Gamper H, Peter M, Jansa J, Lüscher A, Hartwig UA, Leuchtmann A (2004) Arbuscular micorrhizal fungi benefit from seven years of free air CO2 enrichment in well-fertilized grass and legume monocultures. Global Change Biol 10:189–199CrossRefGoogle Scholar
  18. Gamper H, Hartwig UA, Leuchtmann A (2005) Mycorrhizas improve nitrogen nutrition of Trifolium repens after 8 yr of selection under elevated atmospheric CO2 partial pressure. New Phytol (in press)Google Scholar
  19. Gifford RM (1992) Interaction of carbon dioxide with growth-limiting environmental factors in vegetation productivity, implications for the global carbon cycle. In: Desjardins RL, Gifford RM, Nilson T, and Greenwood EAN (eds) Advances in bioclimatology, vol 1. Springer, Berlin Heidelberg New York, pp 24–58Google Scholar
  20. Gloser V, Jezikova M, Lüscher A, Frehner M, Blum H, Nösberger J, Hartwig UA (2000) Soil mineral nitrogen availability was unaffected by elevated atmospheric pCO2 in a four years old field experiment (Swiss FACE). Plant Soil 227:291–299CrossRefGoogle Scholar
  21. Granhall U (1981) Biological nitrogen fixation in relation to environmental factors and functioning of natural ecosystems. In: Clark FE, Rosswall T (eds) Terrestrial nitrogen cycles, processes, ecosystem strategies and management impacts, vol 33. SCOPE Ecological Bulletins, Stockholm, pp 131–144Google Scholar
  22. Hartwig UA (1998) The regulation of symbiotic N2 fixation: a conceptual model of N feedback from the ecosystem to the gene expression level. Perspect Plant Ecol Evol Syst 1:92–120CrossRefGoogle Scholar
  23. Hartwig UA, Zanetti S, Hebeisen T, Lüscher A, Frehner M, Fischer B, Van Kessel C, Hendrey GR, Blum H, Nösberger J (1996) Symbiotic nitrogen fixation: one key to understand the response of temperate grassland ecosystems to elevated CO2? In: Körner C, Bazazz F (eds) Carbon dioxide, populations, and communities. Academic Press, San Diego, pp 253–264Google Scholar
  24. Hartwig UA, Lüscher A, Daepp M, Blum H, Soussana J-F, Nösberger J (2000) Due to symbiotic N2 fixation, five years of elevated atmospheric pCO2 had no effect on litter N concentration in a fertile grassland ecosystem. Plant Soil 224:43–50CrossRefGoogle Scholar
  25. Hartwig UA, Lüscher A, Nösberger J, Kessel C van (2002a) Nitrogen-15 budget in model ecosystems of white clover and perennial ryegrass exposed for four years at elevated atmospheric pCO2. Global Change Biol 8:194–202CrossRefGoogle Scholar
  26. Hartwig UA, Wittmann P, Braun R, Hartwig-Räz B, Jansa J, Mozafar A, Lüscher A, Leuchtmann A, Frossard E, Nösberger J (2002b) Arbuscular mycorrhiza infection enhances the growth response of Lolium perenne to elevated atmospheric pCO2. J Exp Bot 53:1207–1213PubMedCrossRefGoogle Scholar
  27. Hebeisen T, Lüscher A, Zanetti S, Fischer BU, Hartwig UA, Frehner M, Hendrey GR, Blum H, Nösberger J (1997) Growth response of Trifolium repens L. and Lolium perenne L. as monocultures and bi-species mixture to free air CO2 enrichment and management. Global Change Biol 3:149–160CrossRefGoogle Scholar
  28. Ineson P, Coward PA, Hartwig UA (1998) Soil gas fluxes of N2O, CH4 and CO2 beneath Lolium perenne under elevated CO2: the Swiss free air carbon dioxide enrichment experiment. Plant Soil 198:89–95CrossRefGoogle Scholar
  29. Isopp H, Frehner M, Long SP, Nösberger J (2000) Sucrose-phosphate synthase responds differently to source-sink relations and to photosynthetic rates: Lolium perenne L growth at elevated pCO2 in the field. Plant Cell Environ 23:597–607CrossRefGoogle Scholar
  30. Jackson RB, Sala OE, Field CB, Mooney HA (1994) CO2 alters water use, carbon gain, and yield for the dominant species in a natural grassland. Oecologia 98:257–262CrossRefGoogle Scholar
  31. Jacot KA, Lüscher A, Nösberger J, Hartwig UA (2000a) Symbiotic N2 fixation of various legume species along an altitudinal gradient in the Swiss Alps. Soil Biol Biochem 32:1043–1052CrossRefGoogle Scholar
  32. Jacot KA, Lüscher A, Nösberger J, Hartwig UA (2000b) The relative contribution of symbiotic N2 fixation and other nitrogen sources to grassland ecosystems along an altitudinal gradient in the Alps. Plant Soil 225:201–211CrossRefGoogle Scholar
  33. Jongen MB, Jones MB, Hebeisen T, Blum H, Hendrey GR (1995) The effects of elevated CO2 concentrations on the root growth of Lolium perenne and Trifolium repens grown in a FACE system. Global Change Biol 1:361–372CrossRefGoogle Scholar
  34. Lüscher A, Hendrey GR, Nösberger J (1998) Long term responsiveness to free air CO2 enrichment of functional types, species and genotypes of perennial grassland. Oecologia 113:37–45Google Scholar
  35. Lüscher A, Hartwig UA, Suter D, Nösberger J (2000) Direct evidence that in fertile grassland symbiotic N2 fixation is an important trait for a strong response of plants to elevated atmospheric CO2. Global Change Biol 6:655–662CrossRefGoogle Scholar
  36. Montealegre CM, Kessel C van, Blumenthal JM, Hur H-G, Hartwig UA, Sadowsky MJ (2000) Elevated atmospheric CO2 alters microbial population structure in a pasture ecosystem. Global Change Biol 6:475–482CrossRefGoogle Scholar
  37. Montealegre CM, Kessel C van, Russelle MP, Sadowsky MJ (2002) Changes in microbial activity and composition in a pasture ecosystem exposed to elevated atmospheric carbon dioxide. Plant Soil 243:197–207CrossRefGoogle Scholar
  38. Newton PCD, Clark H, Bell CC, Glasgow EM, Campbell BD (1994) Effects of elevated CO2 and simulated seasonal-changes in temperature on the species composition and growth-rate in pasture turves. Ann Bot 73:53–59CrossRefGoogle Scholar
  39. Niklaus PA, Leadley PW, Stöcklin J, Körner C (1998a) Nutrient relations in calcareous grassland under elevated CO2. Oecologia 116:67–75CrossRefGoogle Scholar
  40. Niklaus PA, Spinnler D, Körner C (1998b) Soil moisture dynamics of calcareous grassland under elevated CO2. Oecologia 111:201–208CrossRefGoogle Scholar
  41. Richter M (2003) Influence of elevated atmospheric CO2 concentration on symbiotic N2 fixation and availability of nitrogen in grassland ecosystems. PhD thesis 15185, ETH, Zurich, 125 ppGoogle Scholar
  42. Richter M, Hartwig UA, Frossard E, Cadisch G (2003) Gross fluxes of nitrogen in grassland soil exposed to elevated pCO2 for seven years. Soil Biol Biochem 35:1325–1335CrossRefGoogle Scholar
  43. Rogers A, Fischer BU, Bryant J, Frehner M, Blum H, Raines CA, Long SP (1998) Acclimation of photosynthesis to elevated CO2 under low-nitrogen nutrition is affected by the capacity for assimilate utilization perennial ryegrass under free-air CO2 enrichment. Plant Physiol 118:683–689PubMedCrossRefGoogle Scholar
  44. Schneider MK, Lüscher A, Richter M, Aeschlimann U, Hartwig UA, Blum H, Frossard E, Nösberger J (2004) Ten years of free-air CO2 enrichment altered the mobilisation of N from soil in Lolium perenne L swards. Global Change Biol 10:1377–1388CrossRefGoogle Scholar
  45. Schortemeyer M, Hartwig UA, Hendrey GR, Sadowsky M (1996) Microbial community changes in the rhizospheres of white clover and perennial ryegrass exposed to freeair carbon dioxide enrichment (FACE). Soil Biol Biochem 28:1717–1724CrossRefGoogle Scholar
  46. Schortemeyer M, Atkin OK, McFarlane N, Evans JR (1999) The impact of elevated atmospheric CO2 and nitrate supply on growth, biomass allocation, nitrogen partitioning and N2 fixation of Acacia melanoxylon. Aust J Plant Physiol 26:737–747CrossRefGoogle Scholar
  47. Soussana JF, Hartwig UA (1996) The effects of elevated CO2 on symbiotic N2 fixation: a link between the carbon and nitrogen cycles in grassland ecosystems. Plant Soil 187:321–332CrossRefGoogle Scholar
  48. Soussana JF, Casella E, Loiseau P (1996) Long-term effects of CO2 enrichment and temperature increase on a temperate grass sward II. Plant nitrogen budgets and root fraction. Plant Soil 182:101–114CrossRefGoogle Scholar
  49. Sowerby A, Blum H, Gray TRG, Ball AS (2000) The decomposition of Lolium perenne in soils exposed to elevated CO2: comparisons of mass loss of litter with soil respiration and soil microbial biomass. Soil Biol Biochem 32:1359–1366CrossRefGoogle Scholar
  50. Stöcklin J, Körner C (1999) Interactive effects of elevated CO2, P availability and legume presence on calcareous grassland: results of a glasshouse experiment. Funct Ecol 13:200–209CrossRefGoogle Scholar
  51. Teyssonneyre F, Picon-Cochard C, Falcimagne R, Soussana J-F (2002) Effects of elevated CO2 and cutting frequency on plant community structure in a temperate grassland. Global Change Biol 8:1034–1046CrossRefGoogle Scholar
  52. Thornley JHM, Cannell MGR (1997) Temperate grassland response to climate change: an analysis using the Hurley pasture model. Ann Bot 80:205–221CrossRefGoogle Scholar
  53. Thornley JHM, Cannell MGR (2000) Dynamics of mineral N availability in grassland ecosystems under increased [CO2]: hypothesis evaluated using the Hurley pasture model. Plant Soil 224:153–170CrossRefGoogle Scholar
  54. Van Groeningen KJ, Six J, Harris D, Blum H, Kessel C van (2003) Soil C-13-N-15 dynamics in a N2-fixing clover system under long exposure to elevated atmospheric CO2. Global Change Biol 9:1751–1762CrossRefGoogle Scholar
  55. Vogel CS, Curtis PS, Thomas RB (1997) Growth and nitrogen accretion of dinitrogen-fixing Alnus glutinosa (L) Gaertn. under elevated carbon dioxide. Plant Ecol 130:63–70CrossRefGoogle Scholar
  56. Warwick KR, Taylor G, Blum H (1998) Biomass and compositional changes occur in chalk grassland turves exposed to elevated CO2 for two seasons in FACE. Global Change Biol 4:375–385CrossRefGoogle Scholar
  57. Zanetti S, Hartwig UA (1997) Symbiotic N2 fixation increases under elevated atmospheric pCO2 in the field. Acta Oecol 18:285–290CrossRefGoogle Scholar
  58. Zanetti S, Hartwig U, Lüscher A, Lüscher A, Hebeisen T, Frehner M, Fischer BU, Hendrey GR, Blum H, Nösberger J (1996) Stimulation of symbiotic N2 fixation in Trifolium repens (L) under elevated atmospheric pCO2 in a grassland ecosystem. Plant Physiol 112:575–583PubMedGoogle Scholar
  59. Zanetti S, Hartwig UA, Kessel C van, Lüscher A, Hebeisen T, Frehner M, Fischer BU, Hendrey GR, Blum H, Nösberger J (1997) Does nitrogen nutrition restrict the CO2 response of fertile grassland lacking legumes? Oecologia 112:17–25CrossRefGoogle Scholar
  60. Zanetti S, Hartwig UA, Nösberger J (1998) Elevated atmospheric CO2 does not affect per se the preference for symbiotic nitrogen as opposed to mineral nitrogen of Trifolium repens L. Plant Cell Environ 21:623–630CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • Ueli A. Hartwig
    • 1
  • Michael J. Sadowsky
    • 2
    • 3
  1. 1.Academia EngiadinaSamedanSwitzerland
  2. 2.Department of Soil, Water and ClimateUniversity of MinnesotaSt. PaulUSA
  3. 3.BioTechnology InstituteUniversity of MinnesotaSt. PaulUSA

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