5 Concluding Remarks
Several analytical methods may be used for metabolomic profiling of plants; however, 1H NMR spectra offers a wealth of information of metabolites compared to other methods. Decoupled NMR spectra (J-resolved) provide even more information since metabolites can be accurately integrated and it can exclude broad resonances from macromolecules and spin-spin coupling data. In addition, combinations of two dimensional NMR methods are quite helpful to identify the metabolites in plant extract.
To be able to compare all the data generated from NMR from different experiments and different laboratories, a large database is required. However, that requires a high degree of reproducibility, which can be achieved by using a standardized method for sample preparation and data acquisition.
So far, NMR has been successfully used for the metabolomic fingerprinting and profiling of plants and is successfully applied in quality control of among others, food and botanicals. The use of NMR metabolomics in functional genomics will be the challenge for the coming year.
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Kim, H.K., Choi, Y.H., Verpoorte, R. (2006). Metabolomic Analysis of Catharanthus roseus Using NMR and Principal Component Analysis. In: Saito, K., Dixon, R.A., Willmitzer, L. (eds) Plant Metabolomics. Biotechnology in Agriculture and Forestry, vol 57. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-29782-0_19
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