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Dislocation and Degradation of Proteins from the Endoplasmic Reticulum pp 41–56Cite as

CPY* and the Power of Yeast Genetics in the Elucidation of Quality Control and Associated Protein Degradation of the Endoplasmic Reticulum

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 300))

Abstract

CPY* is a mutated and malfolded secretory enzyme (carboxypeptidase yscY, Gly255Arg), which is imported into the endoplasmic reticulum but never reaches the vacuole, the destination of its wild type counterpart. Its creation, through mutation, had a major impact on the elucidation of the mechanisms of quality control and associated protein degradation of the endoplasmic reticulum, the eukaryotic organelle, where secretory proteins start the passage to their site of action. The use of CPY* and yeast genetics led to the discovery of a new cellular principle, the retrograde transport of lumenal malfolded proteins across the ER membrane back to their site of synthesis, the cytoplasm. These tools furthermore paved the way for our current understanding of the basic mechanism of malfolded protein discovery in the ER and their ubiquitinproteasome driven elimination in the cytosol (ERQD).

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  1. Institut für Biochemie, Universität Stuttgart, Pfaffenwaldring 55, 70569, Stuttgart, Germany

    D. H. Wolf & A. Schäfer

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  1. D. H. Wolf
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  1. Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333, ZA, Leiden, The Netherlands

    Emmanuel Wiertz Ph. D.  & Marjolein Kikkert Ph. D.  & 

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Wolf, D.H., Schäfer, A. (2006). CPY* and the Power of Yeast Genetics in the Elucidation of Quality Control and Associated Protein Degradation of the Endoplasmic Reticulum. In: Wiertz, E., Kikkert, M. (eds) Dislocation and Degradation of Proteins from the Endoplasmic Reticulum. Current Topics in Microbiology and Immunology, vol 300. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-28007-3_3

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