Abstract
The membranes of eukaryotic and prokaryotic cells contain proteins which function as ion channels. The activity of these ion-conducting enzymes is regulated by a variety of physical and chemical factors. In this sense these proteins are ideal components for bio-sensing devices, because they are able to convert the presence of analytes into electrical signals. The present article presents some conceptional and technical aspects on the application of ion channels in bio-sensing. These aspects are discussed in the context of recent advances in the molecular understanding of ion channel structure and function.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Hille B (2001) Ion channels of excitable membranes. 3rd ed, Sinauer Associates, Sunderland
Ashcroft FM (2000) Ion channels and disease. Academic Press, San Diego
Very AA, Sentenac H (2002) Cation channels in the Arabidopsis plasma membrane. Trends Plant Sci 7: 168–175
DiFrancesco D (1993) Pacemaker mechanisms in cardiac tissue. Ann Rev Physiol 55: 455–472
Santoro B, Tibbs GR (1999) The HCN gene family: molecular basis of the hyperpolarization-activated pacemaker channels” Ann NY Acad Sci 868: 741–64
Neher E, Sakmann B (1992) The patch clamp technique. Sci Am 266: 44–51
Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391: 85–100
Colquhoun D, Hawkes AG (1981) On the stochastic properties of single ion channels. Proc R Soc London B 211: 205–235
Miller C (1985) Ion channel reconstitution. Plenum Press, New York
Fertig N, George M, Klau M, Meyer C, Tilke A, Sobotta C, Blick RH, Behrends JC (2003) Microstructured apertures in planar glass substrates for ion channel research. Receptors Channels 9: 29–40
Borisenko V, Lougheed T, Hesse J, Fureder-Kitzmuller E, Fertig N, Behrends JC, Woolley GA, Schutz GJ (2003) Simultaneous optical and electrical recording of single gramicidin channels. Biophys J 84: 612–622
Michalke A et al. (2001) Channel activity of a phytotoxin of Clavibacter michiganense ssp. nebraskense in tethered membranes. Eur Biophys J 3: 421–429
Ide T, Takeuchi Y, Aoki T, Yanagida T (2002) Simultaneous optical and electrical recording of a single ion-channel. Jpn J Physiol 52: 429–434
Ide T, Yanagida T (1999) An artificial lipid bilayer formed on an agarose-coated glass for simultaneous electrical and optical measurement of single ion channels. Biochem Biophys Res Commun 265: 595–599
Lühring H (1999) pH-sensitive gating kinetics of the maxi-K channel in the tonoplast of Chara australis. J Membr Biol 168: 47–61
Papazian DM, Bezanilla F (1999) Voltage-dependent activation of ion channels. Adv Neurol 79: 481–491
Story GM, Peier AM, Reeve AJ, Eid SR, Mosbacher J, Hricik TR, Earley TJ, Hergarden AC, Andersson DA, Hwang SW, McIntyre P, Jegla T, Bevan S, Patapoutian A (2003) ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell 112: 819–829
Liu B, Hui K, Qin F (2003) Thermodynamics of Heat Activation of Single Capsaicin Ion Channels VR1. Biophys J 85: 2988–3006
Kuniyasu A, Kaneko K, Kawahara K, Nakayama H (2003) Molecular assembly and subcellular distribution of ATP-sensitive potassium channel proteins in rat hearts. FEBS Lett 552: 259–263
Dabrowski M, Trapp S, Ashcroft FM (2003) Pyridine nucleotide regulation of the KATP channel Kir6.2/SUR1 expressed in Xenopus oocytes. J Physiol 550: 357–363
Robinson RB, Siegelbaum SA (2003) Hyperpolarization-activated cation currents: from molecules to physiological function. Ann Rev Physiol 65: 453–480
DiFrancesco D, Tortora P (1991): Direct activation of cardiac pacemaker channels by intracellular cyclic AMP. Nature 351: 145–147
DiFrancesco D, Mangoni M (1994) Modulation of single hyperpolarization-activated channels (i(f)) by cAMP in the rabbit sino-atrial node. J Physiol 474:473–482
Sigworth FJ (2003) Life's transistors. Nature 423: 21–22
Stühmer W, Conti F, Suzuki H, Wang XD, Noda M, Yahagi M, Kubo H, Numa S (1989) Structural parts involved in activation and inactivation of the sodium channel. Nature 339: 597–603
Miller AG, Aldrich RW (1996) Conversion of a delayed rectifier K+ channel to a voltage-gated inward rectifier K+ channel by three amino acid substitutions. Neurone 16: 853–858
Grabov A Blatt MR (1997) Parallel control of the inward-rectifier K+ channel by cytosoloic free Ca2+ and pH in Vicia guard cells. Planta 201: 84–95
Hoth S, Hedrich R (1999) Distinct molecular bases for pH sensitivity of the guard cell K+ channels KST1 and KAT1. J Biol Chem 274: 11599–11603
Hoth S, Dreyer I, Dietrich P, Becker D, Muller-Rober B, Hedrich R (1997) Molecular basis of plant-specific acid activation of K+ uptake channels. Proc Natl Acad Sci 94: 4806–4810
Santoro B, Grant SG, Bartsch D, Kandel ER (1997) Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels. Proc Natl Acad Sci 94: 14815–14820
Santoro B, Liu DT, Yao H, Bartsch D, Kandel ER, Siegelbaum SA, Tibbs GR (1998) Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain. Cell 29: 717–729
Ludwig A, Zong X, Stieber J, Hullin R, Hofmann F, Biel M (1998) A family of hyperpolarization-activated mammalian cation channels. Nature 393: 587–591
Shabb JB, Corbin JD (1992) Cyclic nucleotide-binding domains in proteins having diverse functions. J Biol Chem 267: 5723–5726
Wainger BJ et al. (2001) Molecular mechanism of cAMP modulation of HCN pacemaker channels. Nature 411: 805–810
Zagotta WN, Olivier NB, Black KD, Young EC, Olson R, Gouaux E (2003) Structural basis for modulation and agonist specificity of HCN pacemaker channels. Nature 425, 200–205
Chen J, Mitcheson JS, Lin M, Sanguinetti MC (2000) Functional roles of charged residues in the putative voltage sensor of the HCN2 pacemaker channel. J Biol Chem 275: 36465–36471
Vaca L, Stieber J, Zong X, Ludwig A, Hofmann F, Biel M (2000) Mutations in the S4 domain of a pacemaker channel alter its voltage dependence. FEBS Lett. 479: 35–40
Zong X, Stieber J, Ludwig A, Hofmann F, Biel M (2001) A single histidine residue determines the pH sensitivity of the pacemaker channel HCN2. J Biol Chem 276: 6313–6319
Bayley H, Cremer PS (2001) Stochastic sensors inquired by biology. Nature 413: 226–230
Cornell BA et al. (1997) A biosensor that uses ion-channel switches. Nature 387: 580–583
Lucas SW, Harding MM (2000) Detection of DNA via an ion channel switch biosensor. Analyt Biochem 282: 70–79
Movileanu L, Howorka S, Braha O, Bayley H (2000) Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore. Nature Biotechnol 18: 1091–1095
Carrasco L (1995) Modification of membrane permeability by animal viruses. Adv Virus Res 45: 61–112
Fischer WB, Sansom MS (2002) Viral ion channels: structure and function. Biochim Biophys Acta 1561: 27–45
Montal M (2003) Structure-function correlates of Vpu, a membrane protein of HIV-1. FEBS Lett 552: 47–53
Pinto LH, Holsinger LJ, Lamb RA (1992): Influenza virus M2protein has ion channel activity. Cell 69: 517–528
Plugge B, Gazzarrini S, Nelson M, Cerana R, Van Etten JL, Derst C, DiFrancesco D, Moroni A, Thiel G (2000) A potassium channel protein encoded by chlorella virus PBCV-1. Science 287: 1641–1644
Moroni A, Viscomi C, Sangiorgio V, Pagliuca C, Meckel T, Horvath F, Gazzarini S, Valbuzzi P, VanEtten JL, DiFrancesco D, Thiel G (2002) The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv. FEBS Lett 530: 65–69
Gazzarrini S, Severino M, Lombardi M, Morandi M, DiFrancesco D, Van Etten JL, Thiel G, Moroni A (in press) The viral potassium channel Kcv: structural and functional features. FEBS Lett
Gazzarrini S, Van Etten JL, DiFrancesco D, Thiel G, Moroni A. (2002) Voltage-dependence of virus-encoded miniature K+ channel Kcv. J Membrane Biol 187: 15–25
Kang M, Gazzarrini S, Severino M, DiFrancesco D, Thiel G, Van Etten JL, Moroni (submitted) A An insight into domain interactions of a potassium channel pore from natural diversity of viral genes. Molecular Cell Biology
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Thiel, G., Moroni, A. (2005). Ion channels as functional components in sensors of biomedical information. In: Bionik. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26948-7_28
Download citation
DOI: https://doi.org/10.1007/3-540-26948-7_28
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-21890-6
Online ISBN: 978-3-540-26948-9
eBook Packages: Computer Science and Engineering (German Language)