Abstract
As the largest RNA virus, coronavirus replication employs complex mechanisms and involves various viral and cellular proteins. The first open reading frame of the coronavirus genome encodes a large polyprotein, which is processed into a number of viral proteins required for viral replication directly or indirectly. These proteins include the RNA-dependent RNA polymerase (RdRp), RNA helicase, proteases, metal-binding proteins, and a number of other proteins of unknown function. Genetic studies suggest that most of these proteins are involved in viral RNA replication. In addition to viral proteins, several cellular proteins, such as heterogeneous nuclear ribonucleoprotein (hnRNP) A1, polypyrimidine-tract-binding (PTB) protein, poly(A)-binding protein (PABP), and mitochondrial aconitase (m-aconitase), have been identified to interact with the critical cis-acting elements of coronavirus replication. Like many other RNA viruses, coronavirus may subvert these cellular proteins from cellular RNA processing or translation machineries to play a role in viral replication.
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Keywords
- Mouse Hepatitis Virus
- Equine Arteritis Virus
- Murine Coronavirus
- Mouse Hepatitis Virus Strain
- Coronavirus Replication
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Ahola T, den Boon JA, Ahlquist P (2000) Helicase and capping enzyme active site mutations in brome mosaic virus protein 1a cause defects in template recruitment, negative-strand RNA synthesis, and viral RNA capping. J Virol 74:8803–8811
Baker SC, Lai MM (1990) An in vitro system for the leader-primed transcription of coronavirus mRNAs. EMBO J 9:4173–4179
Baker SC, Shieh CK, Soe LH, Chang MF, Vannier DM, Lai MM (1989) Identification of a domain required for autoproteolytic cleavage of murine coronavirus gene A polyprotein. J Virol 63:3693–3699
Baker SC, Yokomori K, Dong S, Carlisle R, Gorbalenya AE, Koonin EV, Lai MM (1993) Identification of the catalytic sites of a papain-like cysteine proteinase of murine coronavirus. J Virol 67:6056–6063
Baker TA, Bell SP (1998) Polymerases and the replisome: machines within machines. Cell 92:295–305
Baric RS, Fu K, Schaad MC, Stohlman SA (1990a) Establishing a genetic recombination map for murine coronavirus strain A59 complementation groups. Virology 177:646–656
Baric RS, Nelson GW, Fleming JO, Deans RJ, Keck JG, Casteel N, Stohlman SA (1988) Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription. J Virol 62:4280–4287
Baric RS, Schaad MC, Wei T, Fu KS, Lum K, Shieh C, Stohlman SA (1990b) Murine coronavirus temperature sensitive mutants. Adv Exp Med Biol 276:349–356
Behrens SE, Tomei L, De Francesco R (1996) Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus. EMBO J 15:12–22
Beinert H, Kennedy MC (1993) Aconitase, a two-faced protein: enzyme and iron regulatory factor. FASEB J 7:1442–1449
Ben-David Y, Bani MR, Chabot B, De Koven A, Bernstein A (1992) Retroviral insertions downstream of the heterogeneous nuclear ribonucleoprotein A1 gene in erythroleukemia cells: evidence that A1 is not essential for cell growth. Mol Cell Biol 12:4449–4455
Bienz K, Egger D, Pfister T (1994) Characteristics of the poliovirus replication complex. Arch Virol Suppl 9:147–157
Bilodeau PS, Domsic JK, Mayeda A, Krainer AR, Stoltzfus CM (2001) RNA splicing at human immunodeficiency virus type 1 3′ splice site A2 is regulated by binding of hnRNP A/B proteins to an exonic splicing silencer element. J Virol 75:8487–8497
Black AC, Luo J, Chun S, Bakker A, Fraser JK, Rosenblatt JD (1996) Specific binding of polypyrimidine tract binding protein and hnRNP A1 to HIV-1 CRS elements. Virus Genes 12:275–285
Black AC, Luo J, Watanabe C, Chun S, Bakker A, Fraser JK, Morgan JP, Rosenblatt JD (1995) Polypyrimidine tract-binding protein and heterogeneous nuclear ribonucleoprotein A1 bind to human T-cell leukemia virus type 2 RNA regulatory elements. J Virol 69:6852–6858
Blackwell JL, Brinton MA (1997) Translation elongation factor-1 alpha interacts with the 3′ stem-loop region of West Nile virus genomic RNA. J Virol 71:6433–6444
Blumenthal T, Carmichael GG (1979) RNA replication: function and structure of Qbeta-replicase. Annu Rev Biochem 48:525–548
Bonilla PJ, Gorbalenya AE, Weiss SR (1994) Mouse hepatitis virus strain A59 RNA polymerase gene ORF 1a: heterogeneity among MHV strains. Virology 198:736–740
Bonilla PJ, Hughes SA, Pinon JD, Weiss SR (1995) Characterization of the leader papain-like proteinase of MHV-A59: identification of a new in vitro cleavage site. Virology 209:489–497
Bonilla PJ, Hughes SA, Weiss SR (1997) Characterization of a second cleavage site and demonstration of activity in trans by the papain-like proteinase of the murine coronavirus mouse hepatitis virus strain A59. J Virol 71:900–909
Bost AG, Carnahan RH, Lu XT, Denison MR (2000) Four proteins processed from the replicase gene polyprotein of mouse hepatitis virus colocalize in the cell periphery and adjacent to sites of virion assembly. J Virol 74:3379–3387
Bost AG, Prentice E, Denison MR (2001) Mouse hepatitis virus replicase protein complexes are translocated to sites of M protein accumulation in the ERGIC at late times of infection. Virology 285:21–29
Bothwell AL, Ballard DW, Philbrick WM, Lindwall G, Maher SE, Bridgett MM, Jamison SF, Garcia-Blanco MA (1991) Murine polypyrimidine tract binding protein. Purification, cloning, and mapping of the RNA binding domain. J Biol Chem 266:24657–24663
Brayton PR, Lai MM, Patton CD, Stohlman SA (1982) Characterization of two RNA polymerase activities induced by mouse hepatitis virus. J Virol 42:847–853
Brayton PR, Stohlman SA, Lai MM (1984) Further characterization of mouse hepatitis virus RNA-dependent RNA polymerases. Virology 133:197–201
Bredenbeek PJ, Pachuk CJ, Noten AF, Charite J, Luytjes W, Weiss SR, Spaan WJ (1990) The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism. Nucleic Acids Res 18:1825–1832
Brierley I, Boursnell ME, Binns MM, Bilimoria B, Blok VC, Brown TD, Inglis SC (1987) An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV. EMBO J 6:3779–3785
Buck KW (1996) Comparison of the replication of positive-stranded RNA viruses of plants and animals. Adv Virus Res 47:159–251
Burd CG, Dreyfuss G (1994) Conserved structures and diversity of functions of RNA-binding proteins. Science 265:615–621
Caputi M, Mayeda A, Krainer AR, Zahler AM (1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J 18:4060–4067
Caputi M, Zahler AM (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J 21:845–855
Chambers TJ, Hahn CS, Galler R, Rice CM (1990) Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44:649–688
Chang RY, Brian DA (1996) cis Requirement for N-specific protein sequence in bovine coronavirus defective interfering RNA replication. J Virol 70:2201–2207
Chang RY, Hofmann MA, Sethna PB, Brian DA (1994) A cis-acting function for the coronavirus leader in defective interfering RNA replication. J Virol 68:8223–8231
Chung RT, Kaplan LM (1999) Heterogeneous nuclear ribonucleoprotein I (hnRNP-I/PTB) selectively binds the conserved 3′ terminus of hepatitis C viral RNA. Biochem Biophys Res Commun 254:351–362
Cologna R, Spagnolo JF, Hogue BG (2000) Identification of nucleocapsid binding sites within coronavirus-defective genomes. Virology 277:235–249
Compton SR, Rogers DB, Holmes KV, Fertsch D, Remenick J, McGowan JJ (1987) In vitro replication of mouse hepatitis virus strain A59. J Virol 61:1814–1820
Craig AW, Haghighat A, Yu AT, Sonenberg N (1998) Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation. Nature 392:520–523
Das T, Mathur M, Gupta AK, Janssen GM, Banerjee AK (1998) RNA polymerase of vesicular stomatitis virus specifically associates with translation elongation factor-1αβγ for its activity. Proc Natl Acad Sci USA 95:1449–1454
De BP, Lesoon A, Banerjee AK (1991) Human parainfluenza virus type 3 transcription in vitro: role of cellular actin in mRNA synthesis. J Virol 65:3268–3275
de la Cruz J, Kressler D, Linder P (1999) Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci 24:192–198
Denison M, Perlman S (1987) Identification of putative polymerase gene product in cells infected with murine coronavirus A59. Virology 157:565–568
Denison MR, Hughes SA, Weiss SR (1995a) Identification and characterization of a 65-kDa protein processed from the gene 1 polyprotein of the murine coronavirus MHV-A59. Virology 207:316–320
Denison MR, Kim JC, Ross T (1995b) Inhibition of coronavirus MHV-A59 replication by proteinase inhibitors. Adv Exp Med Biol 380:391–397
Denison MR, Spaan WJ, van der Meer Y, Gibson CA, Sims AC, Prentice E, Lu XT (1999) The putative helicase of the coronavirus mouse hepatitis virus is processed from the replicase gene polyprotein and localizes in complexes that are active in viral RNA synthesis. J Virol 73:6862–6871
Denison MR, Zoltick PW, Hughes SA, Giangreco B, Olson AL, Perlman S, Leibowitz JL, Weiss SR (1992) Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events. Virology 189:274–284
Dennis DE, Brian DA (1982) RNA-dependent RNA polymerase activity in coronavirus-infected cells. J Virol 42:153–164
Diez J, Ishikawa M, Kaido M, Ahlquist P (2000) Identification and characterization of a host protein required for efficient template selection in viral RNA replication. Proc Natl Acad Sci USA 97:3913–3918
Dong S, Baker SC (1994) Determinants of the p28 cleavage site recognized by the first papain-like cysteine proteinase of murine coronavirus. Virology 204:541–549
Dreyfuss G, Matunis MJ, Pinol-Roma S, Burd CG (1993) hnRNP proteins and the biogenesis of mRNA. Annu Rev Biochem 62:289–321
Froshauer S, Kartenbeck J, Helenius A (1988) Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. J Cell Biol 107:2075–2086
Fu K, Baric RS (1994) Map locations of mouse hepatitis virus temperature-sensitive mutants: confirmation of variable rates of recombination. J Virol 68:7458–7466
Furuya T, Lai MMC (1993) Three different cellular proteins bind to complementary sites on the 5′-end-positive and 3′ end-negative strands of mouse hepatitis virus RNA. J Virol 67:7215–7222
Gallie DR (1998) A tale of two termini: a functional interaction between the termini of an mRNA is a prerequisite for efficient translation initiation. Gene 216:1–11
Gamarnik AV, Andino R (1997) Two functional complexes formed by KH domain containing proteins with the 5′ noncoding region of poliovirus RNA. RNA 3:882–892
Gamarnik AV, Andino R (1998) Switch from translation to RNA replication in a positive-stranded RNA virus. Genes Dev 12:2293–2304
Gao HQ, Schiller JJ, Baker SC (1996) Identification of the polymerase polyprotein products p72 and p65 of the murine coronavirus MHV-JHM. Virus Res 45:101–109
Gontarek RR, Gutshall LL, Herold KM, Tsai J, Sathe GM, Mao J, Prescott C, Del Vecchio AM (1999) hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3′NTR of the HCV RNA genome. Nucleic Acids Res 27:1457–1463
Gorbalenya AE, Blinov VM, Donchenko AP, Koonin EV (1989a) An NTP-binding motif is the most conserved sequence in a highly diverged monophyletic group of proteins involved in positive strand RNA viral replication. J Mol Evol 28:256–268
Gorbalenya AE, Koonin EV (1989) Viral proteins containing the purine NTP-binding sequence pattern. Nucleic Acids Res 17:8413–8440
Gorbalenya AE, Koonin EV (1993) Helicases: amino acid comparisons and structure-function relationships. Curr Opin Struct Biol 3:419–429
Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM (1988) A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination. FEBS Lett 235:16–24
Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM (1989b) Coronavirus genome: prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis. Nucleic Acids Res 17:4847–4861
Gorbalenya AE, Koonin EV, Lai MM (1991) Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi-and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha-and coronaviruses. FEBS Lett 288:201–205
Gorlach M, Burd CG, Dreyfuss G (1994) The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity. Exp Cell Res 211:400–407
Grotzinger C, Heusipp G, Ziebuhr J, Harms U, Suss J, Siddell SG (1996) Characterization of a 105-kDa polypeptide encoded in gene 1 of the human coronavirus HCV 229E. Virology 222:227–235
Gutierrez-Escolano AL, Brito ZU, del Angel RM, Jiang X (2000) Interaction of cellular proteins with the 5′ end of Norwalk virus genomic RNA. J Virol 74:8558–8562
Hahm B, Kim YK, Kim JH, Kim TY, Jang SK (1998) Heterogeneous nuclear ribonucleoprotein L interacts with the 3′ border of the internal ribosomal entry site of hepatitis C virus. J Virol 72:8782–8788
Hamilton BJ, Burns CM, Nichols RC, Rigby WFC (1997) Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation. J Biol Chem 272:28732–28741
Hamilton BJ, Nagy E, Malter JS, Arrick BA, Rigby WFC (1993) Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences. J Biol Chem 268:8881–8887
Harris KS, Xiang W, Alexander L, Lane WS, Paul AV, Wimmer E (1994) Interaction of poliovirus polypeptide 3CDpro with the 5′ and 3′ termini of the poliovirus genome. Identification of viral and cellular cofactors needed for efficient binding. J Biol Chem 269:27004–27014
Hellen CU, Pestova TV, Litterst M, Wimmer E (1994) The cellular polypeptide p57 (pyrimidine tract-binding protein) binds to multiple sites in the poliovirus 5′ nontranslated region. J Virol 68:941–950
Henics T, Sanfridson A, Hamilton BJ, Nagy E, Rigby WFC (1994) Enhanced stability of interleukin-2 mRNA in MLA 144 cells. Possible role of cytoplasmic AU-rich sequence-binding proteins. J Biol Chem 269:5377–5383
Hentze MW, Kuhn LC (1996) Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitric oxide, and oxidative stress. Proc Natl Acad Sci USA 93:8175–8182
Herold J, Gorbalenya AE, Thiel V, Schelle B, Siddell SG (1998) Proteolytic processing at the amino terminus of human coronavirus 229E gene 1-encoded polyproteins: identification of a papain-like proteinase and its substrate. J Virol 72:910–918
Herold J, Siddell SG (1993) An ‘elaborated′ pseudoknot is required for high frequency frameshifting during translation of HCV 229E polymerase mRNA. Nucleic Acids Res 21:5838–5842
Herold J, Siddell SG, Gorbalenya AE (1999) A human RNA viral cysteine proteinase that depends upon a unique Zn2+-binding finger connecting the two domains of a papain-like fold. J Biol Chem 274:14918–14925
Hong Y, Hunt AG (1996) RNA polymerase activity catalyzed by a potyvirus-encoded RNA-dependent RNA polymerase. Virology 226:146–151
Hsue B, Hartshorne T, Masters PS (2000) Characterization of an essential RNA secondary structure in the 3′ untranslated region of the murine coronavirus genome. J Virol 74:6911–6921
Hsue B, Masters PS (1997) A bulged stem-loop structure in the 3′ untranslated region of the genome of the coronavirus mouse hepatitis virus is essential for replication. J Virol 71:7567–7578
Huang P, Lai MM (2001) Heterogeneous nuclear ribonucleoprotein a1 binds to the 3′-untranslated region and mediates potential 5′-3′ end cross talks of mouse hepatitis virus RNA. J Virol 75:5009–5017
Huang P, Lai MMC (1999) Polypyrimidine tract-binding protein binds to the complementary strand of the mouse hepatitis virus 3′ untranslated region, thereby altering RNA conformation. J Virol 73:9110–9116
Huang YT, Romito RR, De BP, Banerjee AK (1993) Characterization of the in vitro system for the synthesis of mRNA from human respiratory syncytial virus. Virology 193:862–867
Hughes SA, Bonilla PJ, Weiss SR (1995) Identification of the murine coronavirus p28 cleavage site. J Virol 69:809–813
Imataka H, Gradi A, Sonenberg N (1998) A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation. EMBO J 17:7480–7489
Ito T, Lai MMC (1997) Determination of the secondary structure of and cellular protein binding to the 3′-untranslated region of the hepatitis C virus RNA genome. J Virol 71:8698–8706
Jackson RJ, Kaminski A (1995) Internal initiation of translation in eukaryotes: the picornavirus paradigm and beyond. RNA 1:985–1000
Jankowsky E, Gross CH, Shuman S, Pyle AM (2000) The DExH protein NPH-II is a processive and directional motor for unwinding RNA. Nature 403:447–451
Jendrach M, Thiel V, Siddell S (1999) Characterization of an internal ribosome entry site within mRNA 5 of murine hepatitis virus. Arch Virol 144:921–933
Joshi RL, Ravel JM, Haenni AL (1986) Interaction of turnip yellow mosaic virus Val-RNA with eukaryotic elongation factor EF-1α. Search for a function. EMBO J 5:1143–1148
Kadare G, Haenni AL (1997) Virus-encoded RNA helicases. J Virol 71:2583–2590
Kaminski A, Hunt SL, Patton JG, Jackson RJ (1995) Direct evidence that polypyrimidine tract binding protein (PTB) is essential for internal initiation of translation of encephalomyocarditis virus RNA. RNA 1:924–938
Kanjanahaluethai A, Baker SC (2000) Identification of mouse hepatitis virus papainlike proteinase 2 activity. J Virol 74:7911–7921
Kennedy MC, Mende-Mueller L, Blondin GA, Beinert H (1992) Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein. Proc Natl Acad Sci USA 89:11730–11734
Kim HY, LaVaute T, Iwai K, Klausner RD, Rouault TA (1996) Identification of a conserved and functional iron-responsive element in the 5′-untranslated region of mammalian mitochondrial aconitase. J Biol Chem 271:24226–24230
Kim JC, Spence RA, Currier PF, Lu X, Denison MR (1995) Coronavirus protein processing and RNA synthesis is inhibited by the cysteine proteinase inhibitor E64d. Virology 208:1–8
Kim KH, Makino S (1995a) Two murine coronavirus genes suffice for viral RNA synthesis. J Virol 69:2313–2321
Kim YN, Jeong YS, Makino S (1993) Analysis of cis-acting sequences essential for coronavirus defective interfering RNA replication. Virology 197:53–63
Kim YN, Makino S (1995b) Characterization of a murine coronavirus defective interfering RNA internal cis-acting replication signal. J Virol 69:4963–4971
Klausner RD, Rouault TA, Harford JB (1993) Regulating the fate of mRNA: the control of cellular iron metabolism. Cell 72:19–28
Koolen MJ, Osterhaus AD, Van Steenis G, Horzinek MC, Van der Zeijst BA (1983) Temperature-sensitive mutants of mouse hepatitis virus strain A59: isolation, characterization and neuropathogenic properties. Virology 125:393–402
Koonin EV, Dolja VV (1993) Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28:375–430
Kuhn LC, Hentze MW (1992) Coordination of cellular iron metabolism by post-transcriptional gene regulation. J Inorg Biochem 47:183–195
Lai MMC (1998) Cellular factors in the transcription and replication of viral RNA genomes: a parallel to DNA-dependent RNA transcription. Virology 244:1–12
Lai MMC, Cavanagh D (1997) The molecular biology of coronaviruses. Adv Virus Res 48:1–100
Landers TA, Blumenthal T, Weber K (1974) Function and structure in ribonucleic acid phage Q beta ribonucleic acid replicase. The roles of the different subunits in transcription of synthetic templates. J Biol Chem 249:5801–5808
Le H, Tanguay RL, Balasta ML, Wei CC, Browning KS, Metz AM, Goss DJ, Gallie DR (1997) Translation initiation factors eIF-iso4G and eIF-4B interact with the poly(A)-binding protein and increase its RNA binding activity. J Biol Chem 272:16247–16255
Lee HJ, Shieh CK, Gorbalenya AE, Koonin EV, La Monica N, Tuler J, Bagdzhadzhyan A, Lai MM (1991) The complete sequence (22 kilobases) of murine coronavirus gene 1 encoding the putative proteases and RNA polymerase. Virology 180:567–582
Leibowitz JL, DeVries JR (1988) Synthesis of virus-specific RNA in permeabilized murine coronavirus-infected cells. Virology 166:66–75
Leibowitz JL, DeVries JR, Haspel MV (1982) Genetic analysis of murine hepatitis virus strain JHM. J Virol 42:1080–1087
Li HP, Huang P, Park S, Lai MMC (1999) Polypyrimidine tract-binding protein binds to the leader RNA of mouse hepatitis virus and serves as a regulator of viral transcription. J Virol 73:772–777
Li HP, Zhang X, Duncan R, Comai L, Lai MMC (1997) Heterogeneous nuclear ribonucleoprotein A1 binds to the transcription-regulatory region of mouse hepatitis virus RNA. Proc Natl Acad Sci USA 94:9544–9549
Liao CL, Lai MMC (1994) Requirement of the 5′ end genomic sequence as an upstream cis-acting element for coronavirus subgenomic mRNA transcription. J Virol 68:4727–4737
Lim KP, Liu DX (1998) Characterization of the two overlapping papain-like proteinase domains encoded in gene 1 of the coronavirus infectious bronchitis virus and determination of the C-terminal cleavage site of an 87-kDa protein. Virology 245:303–312
Lim KP, Ng LF, Liu DX (2000) Identification of a novel cleavage activity of the first papain-like proteinase domain encoded by open reading frame 1a of the coronavirus avian infectious bronchitis virus and characterization of the cleavage products. J Virol 74:1674–1685
Lin YJ, Lai MM (1993) Deletion mapping of a mouse hepatitis virus defective interfering RNA reveals the requirement of an internal and discontiguous sequence for replication. J Virol 67:6110–6118
Lin YJ, Liao CL, Lai MM (1994) Identification of the cis-acting signal for minus-strand RNA synthesis of a murine coronavirus: implications for the role of minus-strand RNA in RNA replication and transcription. J Virol 68:8131–8140
Lin YJ, Zhang X, Wu RC, Lai MMC (1996) The 3′ untranslated region of coronavirus RNA is required for subgenomic mRNA transcription from a defective interfering RNA. J Virol 70:7236–7240
Linder P, Daugeron MC (2000) Are DEAD-box proteins becoming respectable helicases? Nat Struct Biol 7:97–99
Liu DX, Brierley I, Tibbles KW, Brown TD (1994) A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products. J Virol 68:5772–5780
Liu DX, Brown TD (1995) Characterisation and mutational analysis of an ORF 1a-encoding proteinase domain responsible for proteolytic processing of the infectious bronchitis virus 1a/1b polyprotein. Virology 209:420–427
Liu DX, Shen S, Xu HY, Wang SF (1998) Proteolytic mapping of the coronavirus infectious bronchitis virus 1b polyprotein: evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products. Virology 246:288–297
Liu Q, Johnson RF, Leibowitz JL (2001) Secondary structural elements within the 3′ untranslated region of mouse hepatitis virus strain JHM genomic RNA. J Virol 75:12105–12113
Liu Q, Yu W, Leibowitz JL (1997) A specific host cellular protein binding element near the 3′ end of mouse hepatitis virus genomic RNA. Virology 232:74–85
Lohman TM, Bjornson KP (1996) Mechanisms of helicase-catalyzed DNA unwinding. Annu Rev Biochem 65:169–214
Lohmann V, Korner F, Herian U, Bartenschlager R (1997) Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity. J Virol 71:8416–8428
Lu X, Lu Y, Denison MR (1996) Intracellular and in vitro-translated 27-kDa proteins contain the 3C-like proteinase activity of the coronavirus MHV-A59. Virology 222:375–382
Lu XT, Sims AC, Denison MR (1998) Mouse hepatitis virus 3C-like protease cleaves a 22-kilodalton protein from the open reading frame 1a polyprotein in virus-infected cells and in vitro. J Virol 72:2265–2271
Lu Y, Denison MR (1997) Determinants of mouse hepatitis virus 3C-like proteinase activity. Virology 230:335–342
Lu Y, Lu X, Denison MR (1995) Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59. J Virol 69:3554–3559
Luytjes W, Bredenbeek PJ, Noten AF, Horzinek MC, Spaan WJ (1988) Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus. Virology 166:415–422
Ma AS, Moran-Jones K, Shan J, Munro TP, Snee MJ, Hoek KS, Smith R (2002) hnRNP A3, a novel RNA trafficking response element binding protein. J Biol Chem 8:8
Mahy BW, Siddell S, Wege H, ter Meulen V (1983) RNA-dependent RNA polymerase activity in murine coronavirus-infected cells. J Gen Virol 64:103–111
Makino S, Joo M, Makino JK (1991) A system for study of coronavirus mRNA synthesis: a regulated, expressed subgenomic defective interfering RNA results from intergenic site insertion. J Virol 65:6031–6041
Martin JP, Koehren F, Rannou JJ, Kirn A (1988) Temperature-sensitive mutants of mouse hepatitis virus type 3 (MHV-3): isolation, biochemical and genetic characterization. Arch Virol 100:147–160
Mayeda A, Munroe SH, Caceres JF, Krainer AR (1994) Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins. EMBO J 13:5483–5495
Meerovitch K, Svitkin YV, Lee HS, Lejbkowicz F, Kenan DJ, Chan EK, Agol VI, Keene JD, Sonenberg N (1993) La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate. J Virol 67:3798–3807
Michael WM, Siomi H, Choi M, Pinol-Roma S, Nakielny S, Liu Q, Dreyfuss G (1995) Signal sequences that target nuclear import and nuclear export of pre-mRNAbinding proteins. Cold Spring Harb Symp Quant Biol 60:663–668
Miller DJ, Schwartz MD, Ahlquist P (2001) Flock house virus RNA replicates on outer mitochondrial membranes in Drosophila cells. J Virol 75:11664–11676
Molenkamp R, van Tol H, Rozier BC, van der Meer Y, Spaan WJ, Snijder EJ (2000) The arterivirus replicase is the only viral protein required for genome replication and subgenomic mRNA transcription. J Gen Virol 81:2491–2496
Moyer SA, Baker SC, Horikami SM (1990) Host cell proteins required for measles virus reproduction. J Gen Virol 71:775–783
Moyer SA, Baker SC, Lessard JL (1986) Tubulin: a factor necessary for the synthesis of both Sendai virus and vesicular stomatitis virus RNAs. Proc Natl Acad Sci USA 83:5405–5409
Nanda SK, Leibowitz JL (2001) Mitochondrial aconitase binds to the 3′ untranslated region of the mouse hepatitis virus genome. J Virol 75:3352–3362
Nelson GW, Stohlman SA, Tahara SM (2000) High affinity interaction between nucleocapsid protein and leader/intergenic sequence of mouse hepatitis virus RNA. J Gen Virol 81:181–188
Neufeld KL, Richards OC, Ehrenfeld E (1991) Purification, characterization, and comparison of poliovirus RNA polymerase from native and recombinant sources. J Biol Chem 266:24212–24219
Niepmann M (1996) Porcine polypyrimidine tract-binding protein stimulates translation initiation at the internal ribosome entry site of foot-and-mouth-disease virus. FEBS Lett 388:39–42
Niepmann M, Petersen A, Meyer K, Beck E (1997) Functional involvement of polypyrimidine tract-binding protein in translation initiation complexes with the internal ribosome entry site of foot-and-mouth disease virus. J Virol 71:8330–8339
Oglesbee MJ, Liu Z, Kenney H, Brooks CL (1996) The highly inducible member of the 70 kDa family of heat shock proteins increases canine distemper virus polymerase activity. J Gen Virol 77:2125–2135
Osman TA, Buck KW (1997) The tobacco mosaic virus RNA polymerase complex contains a plant protein related to the RNA-binding subunit of yeast eIF-3. J Virol 71:6075–6082
Pardigon N, Strauss JH (1996) Mosquito homolog of the La autoantigen binds to Sindbis virus RNA. J Virol 70:1173–1181
Parsley TB, Towner JS, Blyn LB, Ehrenfeld E, Semler BL (1997) Poly (rC) binding protein 2 forms a ternary complex with the 5′-terminal sequences of poliovirus RNA and the viral 3CD proteinase. RNA 3:1124–1134
Perlman S, Ries D, Bolger E, Chang LJ, Stoltzfus CM (1986) MHV nucleocapsid synthesis in the presence of cycloheximide and accumulation of negative strand MHV RNA. Virus Res 6:261–272
Pinol-Roma S, Dreyfuss G (1992) Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature 355:730–732
Pinon JD, Mayreddy RR, Turner JD, Khan FS, Bonilla PJ, Weiss SR (1997) Efficient autoproteolytic processing of the MHV-A59 3C-like proteinase from the flanking hydrophobic domains requires membranes. Virology 230:309–322
Pinon JD, Teng H, Weiss SR (1999) Further requirements for cleavage by the murine coronavirus 3C-like proteinase: identification of a cleavage site within ORF1b. Virology 263:471–484
Pogue GP, Huntley CC, Hall TC (1994) Common replication strategies emerging from the study of diverse groups of positive-strand RNA viruses. Arch Virol Suppl 9:181–194
Quadt R, Kao CC, Browning KS, Hershberger RP, Ahlquist P (1993) Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase. Proc Natl Acad Sci USA 90:1498–1502
Robb JA, Bond CW (1979) Pathogenic murine coronaviruses. I. Characterization of biological behavior in vitro and virus-specific intracellular RNA of strongly neurotropic JHMVand weakly neurotropic A59V viruses. Virology 94:352–370
Rothstein MA, Richards OC, Amin C, Ehrenfeld E (1988) Enzymatic activity of poliovirus RNA polymerase synthesized in Escherichia coli from viral cDNA. Virology 164:301–308
Sachs AB, Sarnow P, Hentze MW (1997) Starting at the beginning, middle, and end: translation initiation in eukaryotes. Cell 89:831–838
Sawicki SG, Sawicki DL (1986) Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis. J Virol 57:328–334
Schaad MC, Stohlman SA, Egbert J, Lum K, Fu K, Wei T, Jr., Baric RS (1990) Genetics of mouse hepatitis virus transcription: identification of cistrons which may function in positive and negative strand RNA synthesis. Virology 177:634–645
Schalinske KL, Chen OS, Eisenstein RS (1998) Iron differentially stimulates translation of mitochondrial aconitase and ferritin mRNAs in mammalian cells. Implications for iron regulatory proteins as regulators of mitochondrial citrate utilization. J Biol Chem 273:3740–3746
Schiller JJ, Kanjanahaluethai A, Baker SC (1998) Processing of the coronavirus MHV-JHM polymerase polyprotein: identification of precursors and proteolytic products spanning 400 kilodaltons of ORF1a. Virology 242:288–302
Schmid SR, Linder P (1992) D-E-A-D protein family of putative RNA helicases. Mol Microbiol 6:283–291
Schwartz M, Chen J, Janda M, Sullivan M, den Boon J, Ahlquist P (2002) A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids. Mol Cell 9:505–514
Schwarz B, Routledge E, Siddell SG (1990) Murine coronavirus nonstructural protein ns2 is not essential for virus replication in transformed cells. J Virol 64:4784–4791
Seybert A, Hegyi A, Siddell SG, Ziebuhr J (2000) The human coronavirus 229E superfamily 1 helicase has RNA and DNA duplex-unwinding activities with 5′-to-3′ polarity. RNA 6:1056–1068
Shen X, Masters PS (2001) Evaluation of the role of heterogeneous nuclear ribonucleoprotein A1 as a host factor in murine coronavirus discontinuous transcription and genome replication. Proc Natl Acad Sci USA 98:2717–2722
Shi ST, Huang P, Li HP, Lai MMC (2000) Heterogeneous nuclear ribonucleoprotein A1 regulates RNA synthesis of a cytoplasmic virus. EMBO J 19:4701–4711
Shi ST, Schiller JJ, Kanjanahaluethai A, Baker SC, Oh JW, Lai MM (1999) Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells. J Virol 73:5957–5969
Shi ST, Yu GY, Lai MMC (2003) Multiple type A/B heterogeneous nuclear ribonucleoproteins (hnRNPs) can replace hnRNP A1 in mouse hepatitis virus RNA synthesis. J Virol 11:10584–10593
Sims AC, Ostermann J, Denison MR (2000) Mouse hepatitis virus replicase proteins associate with two distinct populations of intracellular membranes. J Virol 74:5647–5654
Siomi H, Dreyfuss G (1995) A nuclear localization domain in the hnRNP A1 protein. J Cell Biol 129:551–560
Snijder EJ, van Tol H, Roos N, Pedersen KW (2001) Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex. J Gen Virol 82:985–994
Sokolowski M, Schwartz S (2001) Heterogeneous nuclear ribonucleoprotein C binds exclusively to the functionally important UUUUU-motifs in the human papillomavirus type-1 AU-rich inhibitory element. Virus Res 73:163–175
Spagnolo JF, Hogue BG (2000) Host protein interactions with the 3′ end of bovine coronavirus RNA and the requirement of the poly(A) tail for coronavirus defective genome replication. J Virol 74:5053–5065
Spangberg K, Wiklund L, Schwartz S (2000) HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3′ ends of hepatitis C virus RNA of both polarities. Virology 274:378–390
Stalcup RP, Baric RS, Leibowitz JL (1998) Genetic complementation among three panels of mouse hepatitis virus gene 1 mutants. Virology 241:112–121
Stohlman SA, Baric RS, Nelson GN, Soe LH, Welter LM, Deans RJ (1988) Specific interaction between coronavirus leader RNA and nucleocapsid protein. J Virol 62:4288–4295
Svitkin YV, Ovchinnikov LP, Dreyfuss G, Sonenberg N (1996) General RNA binding proteins render translation cap dependent. EMBO J 15:7147–7155
Tahara S, Bergmann C, Nelson G, Anthony R, Dietlin T, Kyuwa S, Stohlman S (1993) Effects of mouse hepatitis virus infection on host cell metabolism. Adv Exp Med Biol 342:111–116
Tahara SM, Dietlin TA, Bergmann CC, Nelson GW, Kyuwa S, Anthony RP, Stohlman SA (1994) Coronavirus translational regulation: leader affects mRNA efficiency. Virology 202:621–630
Tahara SM, Dietlin TA, Nelson GW, Stohlman SA, Manno DJ (1998) Mouse hepatitis virus nucleocapsid protein as a translational effector of viral mRNAs. Adv Exp Med Biol 440:313–318
Tan BH, Fu J, Sugrue RJ, Yap EH, Chan YC, Tan YH (1996) Recombinant dengue type 1 virus NS5 protein expressed in Escherichia coli exhibits RNA-dependent RNA polymerase activity. Virology 216:317–325
Tarun SZ, Jr., Sachs AB (1996) Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G. EMBO J 15:7168–7177
Tarun SZ, Jr., Wells SE, Deardorff JA, Sachs AB (1997) Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation. Proc Natl Acad Sci USA 94:9046–9051
Thiel V, Herold J, Schelle B, Siddell SG (2001) Viral replicase gene products suffice for coronavirus discontinuous transcription. J Virol 75:6676–6681
Thiel V, Siddell SG (1994) Internal ribosome entry in the coding region of murine hepatitis virus mRNA 5. J Gen Virol 75:3041–3046
Tijms MA, van Dinten LC, Gorbalenya AE, Snijder EJ (2001) A zinc finger-containing papain-like protease couples subgenomic mRNA synthesis to genome translation in a positive-stranded RNA virus. Proc Natl Acad Sci USA 98:1889–1894
Valcarcel J, Gebauer F (1997) Post-transcriptional regulation: the dawn of PTB. Curr Biol 7:R705–708
van der Meer Y, Snijder EJ, Dobbe JC, Schleich S, Denison MR, Spaan WJ, Locker JK (1999) Localization of mouse hepatitis virus nonstructural proteins and RNA synthesis indicates a role for late endosomes in viral replication. J Virol 73:7641–7657
van Dinten LC, den Boon JA, Wassenaar AL, Spaan WJ, Snijder EJ (1997) An infectious arterivirus cDNA clone: identification of a replicase point mutation that abolishes discontinuous mRNA transcription. Proc Natl Acad Sci USA 94:991–996
van Dinten LC, Rensen S, Gorbalenya AE, Snijder EJ (1999) Proteolytic processing of the open reading frame 1b-encoded part of arterivirus replicase is mediated by nsp4 serine protease and Is essential for virus replication. J Virol 73:2027–2037
van Dinten LC, Wassenaar AL, Gorbalenya AE, Spaan WJ, Snijder EJ (1996) Processing of the equine arteritis virus replicase ORF1b protein: identification of cleavage products containing the putative viral polymerase and helicase domains. J Virol 70:6625–6633
Van Dyke TA, Flanegan JB (1980) Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase. J Virol 35:732–740
Wang Y, Zhang X (1999) The nucleocapsid protein of coronavirus mouse hepatitis virus interacts with the cellular heterogeneous nuclear ribonucleoprotein A1 in vitro and in vivo. Virology 265:96–109
Wang YF, Chen SC, Wu FY, Wu CW (1997) The interaction between human cytomegalovirus immediate-early gene 2 (IE2) protein and heterogeneous ribonucleoprotein A1. Biochem Biophys Res Commun 232:590–594
Weighardt F, Biamonti G, Riva S (1995) Nucleo-cytoplasmic distribution of human hnRNP proteins: a search for the targeting domains in hnRNP A1. J Cell Sci 108:545–555
Williams GD, Chang RY, Brian DA (1995) Evidence for a pseudoknot in the 3′ untranslated region of the bovine coronavirus genome. Adv Exp Med Biol 380:511–514
Wu-Baer F, Lane WS, Gaynor RB (1996) Identification of a group of cellular cofactors that stimulate the binding of RNA polymerase II and TRP-185 to human immunodeficiency virus 1 TAR RNA. J Biol Chem 271:4201–4208
Yokomori K, Banner LR, Lai MM (1991) Heterogeneity of gene expression of the hemagglutinin-esterase (HE) protein of murine coronaviruses. Virology 183:647–657
Yokomori K, Lai MM (1991) Mouse hepatitis virus S RNA sequence reveals that nonstructural proteins ns4 and ns5a are not essential for murine coronavirus replication. J Virol 65:5605–5608
Yu W, Leibowitz JL (1995a) A conserved motif at the 3′ end of mouse hepatitis virus genomic RNA required for host protein binding and viral RNA replication. Virology 214:128–138
Yu W, Leibowitz JL (1995b) Specific binding of host cellular proteins to multiple sites within the 3′ end of mouse hepatitis virus genomic RNA. J Virol 69:2016–2023
Yuan ZH, Kumar U, Thomas HC, Wen YM, Monjardino J (1997) Expression, purification, and partial characterization of HCV RNA polymerase. Biochem Biophys Res Commun 232:231–235
Zhang X, Lai MMC (1995a) Interactions between the cytoplasmic proteins and the intergenic (promoter) sequence of mouse hepatitis virus RNA: correlation with the amounts of subgenomic mRNA transcribed. J Virol 69:1637–1644
Zhang X, Li HP, Xue W, Lai MMC (1999) Formation of a ribonucleoprotein complex of mouse hepatitis virus involving heterogeneous nuclear ribonucleoprotein A1 and transcription-regulatory elements of viral RNA. Virology 264:115–124
Zhang X, Liao CL, Lai MMC (1994) Coronavirus leader RNA regulates and initiates subgenomic mRNA transcription both in trans and in cis. J Virol 68:4738–4746
Zhang XM, Lai MMC (1995b) Regulation of coronavirus RNA transcription is likely mediated by protein-RNA interactions. Adv Exp Med Biol 380:515–521
Ziebuhr J, Siddell SG (1999) Processing of the human coronavirus 229E replicase polyproteins by the virus-encoded 3C-like proteinase: identification of proteolytic products and cleavage sites common to pp1a and pp1ab. J Virol 73:177–185
Ziebuhr J, Snijder EJ, Gorbalenya AE (2000) Virus-encoded proteinases and proteolytic processing in the Nidovirales. J Gen Virol 81:853–879
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Shi, S.T., Lai, M.M.C. (2005). Viral and Cellular Proteins Involved in Coronavirus Replication. In: Enjuanes, L. (eds) Coronavirus Replication and Reverse Genetics. Current Topics in Microbiology and Immunology, vol 287. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-26765-4_4
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