Phospholipide reduzieren die intraperitoneale Tumorzelladhäsion in Ratten

Abstract

Introduction: Adhesion of tumor cells to the peritoneum is a pivotal step in developing peritoneal dissemination after resection of colorectal cancer. Adhesion is promoted by integrins. We assessed the inhibition of cell attachment by local treatment with phospholipids to the peritoneum in rats as well as the integrin expression on tumor cells. Methods: In 40 BD-IX rats colinic cancer cells DHD/ K12-TRb were injected intraperitoneally suspended in either normal saline (controls) or phospholipid 9% (PL). After 30 days the extent of peritoneal carcinosis (mm², ml) and the Peritoneal Cancer Index were evaluated. Additionally the expression of alpha3beta1 Integrin (collagenlaminin-receptor) on the surface of tumor cells was analysed after treatment with phospholipids by Flowcytometry (mean fluorescence/400.000 cells). Wilcoxon-Test was used for statistical assessment. Results: The amount of peritoneal cancer after 30 days could be reduced significantly after treating the tumor cells with PL 9% (p < 0.001) (¤ Table 1). The mean survival was prolonged in the treatment group (50.1 ± 17.4 days compared to controls (43.9 ± 4.8 days). Expression of alpha3-integrin (beta1-integrin) on the surface of tumor cells was reduced by PL 9% to an amount of 123 ± 48 (568 ± 176) compared to 252 ± 64 (698 ± 229) in the control group. Conclusion: Phospholipids may be an efficacious tool to reduce peritoneal tumour cell adhesion and consecutively the development of peritoneal carcinosis caused by free intraperitoneal tumor cells after resection of colorectal cancer.