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Enzyme regulation in parsley and soybean cell cultures

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Book cover Advances in Biomedical Engineering, Volume 18

Part of the book series: Advances in Biochemical Engineering ((ABE,volume 18))

Abstract

Cell suspension cultures of parsley (Petroselinum hortense) and soybean (Glycine max) have been used for several years as model systems for enzyme regulation studies. The regulation of nitrate metabolism, of general phenylpropanoid metabolism, and of flavonoid and lignin biosynthesis have been investigated. The results obtained for these metabolic pathways in the two cultures are compared and discussed with regard to changes in various parameters related to the growth cycle of the cultures. The parameters measured include the rate of cell division, DNA, RNA, and protein concentrations, and the rates of protein synthesis and degradation.

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Abbreviations

ACC:

Acetyl-CoA carboxylase (EC 6.4.1.2)

APT:

UDP-Apiose: flavone 7-O-glucoside 2″-O-apiosyltransferase (EC 2.4.2.25)

C4H:

Cinnamate 4-hydroxylase (EC 1.14.13.11)

CHI:

Chalcone isomerase (EC 5.5.1.6)

CHS:

Chalcone synthase (previous name: ‘flavanone synthase’)

4CL:

4-Hydroxycinnamate: CoA ligase (EC 6.2.1.12)

3GT:

UDP-Glucose: flavonol 3-O-glucosyltransferase (EC 2.4.1.-)

7GT:

UDP-Glucose: flavonoid 7-O-glucosyltransferase (EC 2.4.1.82)

MAT:

Malonyl-CoA: flavonoid glycoside malonyltransferase (EC 2.3.1.-)

3'MT:

S-Adenosyl-L-methionine:flavonoid 3'-O-methyltransferase (EC 2.1.1.42)

PAL:

Phenylalanine ammonialyase (EC 4.3.1.5)

RED1:

Cinnamoyl-CoA: NADPH oxidoreductase (EC 1.1.1.-)

RED2:

Cinnamyl alcohol: NADP oxidoreductase (EC 1.1.1.- )

UAS:

UDP-Apiose synthase

kat (katal):

unit of enzyme activity: the amount of activity that converts one mole of substrate per second. According to the Recommendations of the Commission on Biochemical Nomenclature, the specific activity is expressed as kat/kg protein

mho( = S):

conductivity

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Authors and Affiliations

  1. Biologisches Institut II der Universität, Schänzlestr. 1, 7800, Freiburg i.Br., Germany

    Hahlbrock, J. Schroder & J. Vieregge

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  1. Hahlbrock
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  2. J. Schroder
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  3. J. Vieregge
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A. Fiechter

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© 1980 Springer Verlag

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Hahlbrock, Schroder, J., Vieregge, J. (1980). Enzyme regulation in parsley and soybean cell cultures. In: Fiechter, A. (eds) Advances in Biomedical Engineering, Volume 18. Advances in Biochemical Engineering, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-09936-0_2

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  • DOI: https://doi.org/10.1007/3-540-09936-0_2

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  • Print ISBN: 978-3-540-09936-9

  • Online ISBN: 978-3-540-39074-9

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