Skip to main content
SpringerLink
Log in

Preparation and properties of gel entrapped enzymes

  • Conference paper
  • First Online:
Advances in Biochemical Engineering, Volume 4

Part of the book series: Advances in Biochemical Engineering ((ABE,volume 4))

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

D:

diffusion coefficient of substrate in gel

[E]gel :

active enzyme concentration per unit volume of gel

F:

function defined by Eq. (3)

H:

fractional extent of hydiation of gel

k ′c :

catalytic constant of enzyme in gel

K ′m :

Michaelis constant of enzyme in gel

l:

membrane thickness

M:

molecular weight of substrate

R:

radius of spherical gel particles

[S]:

substrate concentration in gel

υ ′ :

rate of enzyme catalyzed reaction per unit volume of gel

α:

defined by Eq. (4)

β:

Damköhler number =α 2 l 2, or =α 2 R 2

η:

effectiveness factor, i. e.: rate catalyzed by gel entrapped enzyme relative to rate of free enzyme under equivalent conditions

φ:

Thiele modulus (Eq. 6)

References

  1. “Immobilized Enzymes: A Compendium of References...” Corning Glass Works, Corning, N. Y., 1972.

    Google Scholar 

  2. Zaborsky, O. R.: “Immobilized Enzymes” CRC Press, Cleveland O., 1973.

    Google Scholar 

  3. Yasuda, H., Peterlin, A., Colton, C. K., Smith, K. A., Merrill, E. W.: Makromol. Chem. 126, 177 (1969).

    Article  CAS  Google Scholar 

  4. Yasuda, H., Lamaze, C. E., Peterling, A.: J. Polymer Sci. A-2, 9 1117 (1971).

    Article  CAS  Google Scholar 

  5. Bernfeld, P., Wan, J.: Science 142, 678, (1963).

    Article  CAS  Google Scholar 

  6. O'Driscoll, K. F., Izu, M., Korus, R.: Biotechnol. Bioeng. 14, 847, (1972).

    Article  Google Scholar 

  7. Miyamura, M., Suzuki, S.: Nippon Kagaku Kaishi, 7, 1274, (1972).

    Google Scholar 

  8. Turkova, J., Hubalkova, O., Krivakova, M., Coupek, J.: Biochim. Biophys. Acta. 322, 1, (1973).

    CAS  Google Scholar 

  9. Gould, F. E., Ronel, S. H.: Germ. Pat. No. 2, 305, 320 (1973).

    Google Scholar 

  10. Degani, Y., Miron, T.: Biochim. Biophys. Acta. 212, 362, (1970).

    CAS  Google Scholar 

  11. Korus, R., Ph. D. Thesis: University of Waterloo, Waterloo, Ontario, (1974).

    Google Scholar 

  12. O'Driscoll, K. F., Kapoulas, A.: unpublished work.

    Google Scholar 

  13. Nilsson, H., Mosbach, R., Mosbach, K.: Biochim. Biophys. Acta 268, 253, (1972).

    CAS  Google Scholar 

  14. Beck, S. R., Rase, H. F.: Ind. Eng. Chem. Prod. Res. Develop. 12, 260, (1973).

    Article  CAS  Google Scholar 

  15. Mosbach, K., Mosbach, R.: Acta Chem. Scand., 20, 2807, (1966).

    Article  CAS  Google Scholar 

  16. Mosbach, K., Larsson, P.-O.: Biotechnol. Bioeng. 12, 19, (1970).

    Article  CAS  Google Scholar 

  17. Slowinski, W., Charm, S. E.: Biotechnol. and Bioeng. 15, 973, (1973).

    Article  CAS  Google Scholar 

  18. Turkova, J., Hubalkova, O., Krivakova, M., Coupek, J.: Biochim. Biophys. Acta. 322, 1, (1973).

    CAS  Google Scholar 

  19. Dobo, J.: Acta. Chim. Acad. Sci. Hung. 63, 453, (1970) through CA 72: 96979 m (1970).

    CAS  Google Scholar 

  20. Maeda, H., Yamauchi, A., Suzuki, H.: Biochim. Biophys. Acta. 315, 18, (1973).

    CAS  Google Scholar 

  21. Dickey, F. H.: J. Phys. Chem., 59, 695, (1955).

    Article  CAS  Google Scholar 

  22. Johnson, P., Whateley, T. L.: J. Colloid Interface Sci. 37, 557, (1971).

    Article  CAS  Google Scholar 

  23. Brown, H. D., Patel, A. B., Chattopadhyay, S. K.: J. Biomed. Mater. Res. 2, 231, (1968).

    Article  CAS  Google Scholar 

  24. Pennington, S. N., Brown, H. D., Patel, A. B., Knowles, C. O.: Biochim. Biophys. Acta. 167, 479, (1968).

    CAS  Google Scholar 

  25. Pennington, S. N., Brown, H. D., Patel, A. B., Chattopadhyay, S. K.: J. Biomed. Mater. Res. 2, 443,(1968).

    Article  CAS  Google Scholar 

  26. Guilbault, G. G., Das, J.: Anal. Biochem. 33, 341, (1970).

    Article  CAS  Google Scholar 

  27. Bauman, E. K., Goodson, L. H., Guilbault, G. G., Kramer, D. N.: Anal. Chem. 37, 1378 (1965).

    Article  CAS  Google Scholar 

  28. Guilbault, G. G., Kramer, D. N.: Anal. Chem., 37, 1675, (1965).

    Article  CAS  Google Scholar 

  29. Maeda, H., Suzuki, H., Yamauchi, A.: Biotechnol. Bioeng. 15, 607, 827, (1973).

    Article  CAS  Google Scholar 

  30. Horvath, C, Sovak, M.: Biochim. Biophys. Acta. 298, 850, (1973).

    Article  CAS  Google Scholar 

  31. Wang, S. S., Vieth, W. R.: Biotechnol. Bioeng. 15, 93, (1973).

    Article  CAS  Google Scholar 

  32. Brown, G., Thomas, D., Domurado, D., Berjonneau, A., Guillon, G.: Biotechnol. Bioeng. 15, 359,(1973).

    Article  Google Scholar 

  33. Chang, T. M. S.: Science 146, 524 (1964).

    Article  CAS  Google Scholar 

  34. Chang, T. M. S.: “Artificial Cells”, C. C. Thomas Publ., Springfield III., (1972).

    Google Scholar 

  35. Gregoriadis, G., Leathwood, P. D., Ryman, B. E.: FEBS Letters 14, 95, (1971).

    Article  CAS  Google Scholar 

  36. Dinish, K. N., Shivaram, K. N., Stegeman, H.: Z. Anal. Chem. 263, 27, (1973).

    Article  CAS  Google Scholar 

  37. Dinelli, D.: Process Biochemistry 7(8), 13, (1972).

    Google Scholar 

  38. Kobayashi, T., Laidler, K. J.: Biotechnol. Bioeng. 16, 77, (1974).

    Article  CAS  Google Scholar 

  39. Smith, N. L., Amundson, N. R.: Ind. and Eng. Chem. 43, 2156, (1951).

    Article  CAS  Google Scholar 

  40. Hamilton, B. K., Gardner, C. R., Colton, C. K.: A. E. Ch. E. J. 20, 503, (1974).

    Article  CAS  Google Scholar 

  41. Goldman, R., Kedem, O., Katchalski, E.: Biochem. 7, 4518, (1968).

    Article  CAS  Google Scholar 

  42. Sundaram, P. V., Tweedale, A., Laidler, K. J.: Can. J. Chem. 48, 1498, (1970).

    Article  CAS  Google Scholar 

  43. O'Driscoll, K. F., Hinberg, I., Korus, R., Kapoulas, A.: J. Polymer Sci. C46, 227 (1974).

    Google Scholar 

  44. Moo-Young, M., Kobayashi, T.: Can. J. Chem. Eng. 50, 162, (1972).

    Article  CAS  Google Scholar 

  45. Fink, D. J., Na., T. Y., Schultz, J. S.: Biotechnol Bioeng. 15, 879, (1973).

    Article  CAS  Google Scholar 

  46. Engasser, J. M., Horvath, C: J. Theor. Biol. 42, 137, (1973).

    Article  CAS  Google Scholar 

  47. O'Driscoll, K. F., Korus, R.: Can. J. Chem. Eng. 52, N 775 (1974).

    Google Scholar 

  48. Goldman, R., Goldstein, L., Katchalski, E.: “Biochemical Aspects of Reaction on Solid Supports”, G. R. Stark, Ed., Chap. 1, Acad. Press, N. Y. (1971).

    Google Scholar 

  49. Hinberg, I., Kapoulas, A., Korus, R., O'Driscoll, K.: Biotechnol. Bioeng. 16, 159, (1974).

    Article  CAS  Google Scholar 

  50. Ollis, D. F.: Biotechnol. Bioeng. 14, 871, (1972).

    Article  CAS  Google Scholar 

  51. Horvath, C, Engasser, J. M: Ind. Eng. Chem. Fundam. 12, 229, (1973).

    Article  CAS  Google Scholar 

  52. Regan, D. L., Dunnill, P., Lilly, M. D.: Biotechnol. Bioeng. 16, 333 (1974).

    Article  CAS  Google Scholar 

  53. Brown, H. D., Patel, A. B., Chattopadhyay, S. K.: J. Chromatogr., 35, 103, (1968).

    Article  CAS  Google Scholar 

  54. Broun, G., Thomas, D., Selegny, E.: J. Membrane Biol. 8, 313, (1972).

    Article  CAS  Google Scholar 

  55. Mosbach, K., Mattiasson, B.: Acta Chem. Scand. 24, 2093, (1970).

    Article  CAS  Google Scholar 

  56. Brown, H. D., Patel, A. B., Chattopadhyay, S. K.: J. Chromatogr. 35, 103, (1968).

    Article  CAS  Google Scholar 

  57. Mattiason, B., Mosbach, K.: Biochim. Biophys. Acta 235, 253, (1971).

    Google Scholar 

  58. Gestrelius, S., Mattiasson, B., Mosbach, K.: Biochim. Biophys. Acta 276, 339, (1972).

    CAS  Google Scholar 

  59. Gestrelius, S., Mattiasson, B., Mosbach, K.: Euro. J. Biochem 36, 89, (1973).

    Article  CAS  Google Scholar 

  60. Srere, P. A., Mattiasson, B., Mosbach, K.: Proc. Nat. Acad. Sci. U.S.A. 70, 2534, (1973).

    Article  CAS  Google Scholar 

  61. Lawrence, R. L., Okay, V.: Biotechn. Bioeng. 15, 217, (1973).

    Article  CAS  Google Scholar 

  62. von der Ploeg, M., van Duijn, P.: J. Roy. Microscop. Soc. 83, 415, (1964).

    Google Scholar 

  63. von Duijn, P., Pascoe, E., von der Ploeg, M.: J. Histochem. Cytochem. 15, 631, (1967).

    Google Scholar 

  64. Hicks, G. P., Updike, S. J.: Anal. Chem. 38, 762, (1966), “also U. S. Patent 3, 788, 950 (1974).”

    Article  Google Scholar 

  65. Gough, D. A., Amdrake, J. D.: Science 180, 380, (1973).

    Article  CAS  Google Scholar 

  66. Towshend, A.: Process Biochemistry, 8 (3), 23, (1973).

    Google Scholar 

  67. “Enzyme Therapy in Genetic Diseases”, D. Bergsma Ed., National Foundation — March of Dimes, White Plains, N. Y., (1973).

    Google Scholar 

  68. Chang, T. M. S.: Enzyme 14, 95, (1972/73).

    Google Scholar 

  69. Ohnuma, T., O'Driscoll, K., Korus, R., Wolczak, I.: J. Pharm. Exper. Therap. 195, 382 (1975).

    Google Scholar 

  70. Updike, S., Prieve, C, Magnuson, J.: Enzyme 14, 77, (1972/73).

    Google Scholar 

  71. Rubin, D. H.: “A Technology Assessment Methodology: Enzymes (Industrial)”, National Technical Information Service, Springfield, Va., (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada

    F. O'Driscoll

Authors
  1. F. O'Driscoll
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1976 Springer-Verlag

About this paper

Cite this paper

O'Driscoll, F. (1976). Preparation and properties of gel entrapped enzymes. In: Advances in Biochemical Engineering, Volume 4. Advances in Biochemical Engineering, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-07747-2_8

Download citation

  • DOI: https://doi.org/10.1007/3-540-07747-2_8

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-07747-3

  • Online ISBN: 978-3-540-38240-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation