Summary
NF-κB is a nuclear transcription factor involved in the control of fundamental cellular functions including regulation of cell survival. We investigated NF-κB activation induced by two opposing modulators of cell viability: IL-1β and glutamate. We found that IL-1β activated p50, p65 and c-Rel subunits of NF-κB, while glutamate activated only p50 and p65 proteins. Cell stimulation by glutamate, correlated with expression of the pro-apoptotic genes Caspase-3, Caspase-2L and Bax. Conversely, IL-1β induced the expression of the short anti-apoptotic isoform of Caspase-2. Finally, we analysed the effect of the inhibition of IκBα degradation on glutamate-induced toxicity by using BAY 11-7082, a selective inhibitor of IκBα phosphorylation. Our results suggest that BAY 11-7082 preserves neuron viability from the glutamate-mediated injury.
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Pizzi, M., Sarnico, I., Boroni, F., Benetti, A., Benarese, M., Spano, P.F. (2005). Inhibition of IκBα phosphorylation prevents glutamate-induced NF-κB activation and neuronal cell death. In: von Wild, K.R.H. (eds) Re-Engineering of the Damaged Brain and Spinal Cord. Acta Neurochirurgica Supplementum, vol 93. Springer, Vienna. https://doi.org/10.1007/3-211-27577-0_8
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DOI: https://doi.org/10.1007/3-211-27577-0_8
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-24150-9
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