Abstract
A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 °C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 °C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.
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© 2008 Springer-Verlag Berlin Heidelberg
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González-Pérez, A., Bulut, S., Olsson, U., Lindman, B. (2008). Temperature Induced DNA Compaction in a Nonionic Lamellar Phase. In: Hórvölgyi, Z.D., Kiss, É. (eds) Colloids for Nano- and Biotechnology. Progress in Colloid and Polymer Science, vol 135. Springer, Berlin, Heidelberg. https://doi.org/10.1007/2882_2008_106
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DOI: https://doi.org/10.1007/2882_2008_106
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