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Multispectral Optoacoustic Tomography of Brown Adipose Tissue

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Brown Adipose Tissue

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 251))

Abstract

MSOT has revolutionized biomedical imaging because it allows anatomical, functional, and molecular imaging of deep tissues in vivo in an entirely noninvasive, label-free, and real-time manner. This imaging modality works by pulsing light onto tissue, triggering the production of acoustic waves, which can be collected and reconstructed to provide high-resolution images of features as deep as several centimeters below the body surface. Advances in hardware and software continue to bring MSOT closer to clinical translation. Most recently, a clinical handheld MSOT system has been used to image brown fat tissue (BAT) and its metabolic activity by directly resolving the spectral signatures of hemoglobin and lipids. This opens up new possibilities for studying BAT physiology and its role in metabolic disease without the need to inject animals or humans with contrast agents. In this chapter, we overview how MSOT works and how it has been implemented in preclinical and clinical contexts. We focus on our recent work using MSOT to image BAT in resting and activated states both in mice and humans.

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Author notes

  1. Angelos Karlas and Josefine Reber contributed equally to this work.

Authors and Affiliations

  1. Chair of Biological Imaging, Technical University Munich, Munich, Germany

    Angelos Karlas, Josefine Reber, Evangelos Liapis, Korbinian Paul-Yuan & Vasilis Ntziachristos

  2. Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany

    Angelos Karlas, Josefine Reber, Evangelos Liapis, Korbinian Paul-Yuan & Vasilis Ntziachristos

Authors
  1. Angelos Karlas
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  2. Josefine Reber
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  3. Evangelos Liapis
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  4. Korbinian Paul-Yuan
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  5. Vasilis Ntziachristos
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Corresponding author

Correspondence to Vasilis Ntziachristos .

Editor information

Editors and Affiliations

  1. Institute of Pharmacology & Toxicology, University Hospital Bonn, University of Bonn, Bonn, Germany

    Alexander Pfeifer

  2. Institute of Molecular Nutritional Medicine, TU München, Freising, Bayern, Germany

    Martin Klingenspor

  3. Institute for Diabetes and Cancer IDC, Helmholtz Center München, Neuherberg, Germany

    Stephan Herzig

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Karlas, A., Reber, J., Liapis, E., Paul-Yuan, K., Ntziachristos, V. (2018). Multispectral Optoacoustic Tomography of Brown Adipose Tissue. In: Pfeifer, A., Klingenspor, M., Herzig, S. (eds) Brown Adipose Tissue. Handbook of Experimental Pharmacology, vol 251. Springer, Cham. https://doi.org/10.1007/164_2018_141

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