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Polymer Complexes in Biological Applications

  • Jana Hedrich
  • Yuzhou Wu
  • Seah Ling Kuan
  • Frauke Kuehn
  • Eweline Pietrowski
  • Mike Sahl
  • Sandra Muth
  • Klaus Müllen
  • Heiko J. Luhmann
  • Tanja Weil
  • Manfred SchmidtEmail author
Chapter
Part of the Advances in Polymer Science book series (POLYMER, volume 260)

Abstract

This chapter summarizes the influence of polyelectrolyte topology on biological functions and biomedical applications such as cell uptake, drug delivery, and gene transfection. Polyelectrolytes utilized are spherical structures derived from dendrimers and albumin or cylindrical brushes, all of which are decorated with various polypeptide chains.

First, experiments are described that address the role of polyelectrolyte interactions on endosomal uptake and release, followed by a discussion of the novel design of albumin-based nanocarriers for anticancer drugs like doxorubicin. Finally, we describe how efficient gene transfection was accomplished with both albumin-based polycations and with cylindrical brushes having poly-l-lysine side chains.

Keywords

Brain Cylindrical brush polymer Dendritic and protein polyelectrolytes Endothelial cell Flow cytometry Gene transfer Polycation Transfection 

Abbreviations

AML

Acute myeloid leukemia

ATRP

Atom transfer radical polymerization

cBSA

Cationized bovine serum albumin

DHSA

Dendronized human serum albumin

DMF

Dimethyl formamide

DOX

Doxorubicin

ECM

Extracellular matrix

EPR

Enhanced retention and permeation

GFP

Green fluorescent protein

HSA

Human serum albumin

LiBr

Lithium bromide

MRI

Magnetic resonance imaging

Mw

Weight-average molecular weight

NaCl

Sodium chloride

PAMAM

Polyamidoamine

PBMEC

Porcine microvascular endothelial cells

PDI

Perylene-3,4,9,10-tetracarboxdiimide

PEG

Poly(ethylene glycol)

PEI

Poly(ethylene imine)

PEO

Poly(ethylene oxide)

PLL

Poly-l-lysine

Pn

Number-average degree of polymerization

Pw

Weight-average degree of polymerization

Rg

Radius of gyration

Rh

Hydrodynamic radius

TEM

Transmission electron microscopy

wDNA

Weight fraction of DNA

z+/z

Molar charge ratio polycation/polyanion

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jana Hedrich
    • 1
  • Yuzhou Wu
    • 2
  • Seah Ling Kuan
    • 2
  • Frauke Kuehn
    • 3
  • Eweline Pietrowski
    • 1
  • Mike Sahl
    • 3
  • Sandra Muth
    • 3
  • Klaus Müllen
    • 4
  • Heiko J. Luhmann
    • 1
  • Tanja Weil
    • 2
  • Manfred Schmidt
    • 3
    Email author
  1. 1.Institute of Physiology and PathophysiologyUniversity Medical CenterMainzGermany
  2. 2.Institute of Organic Chemistry IIIUniversity of UlmUlmGermany
  3. 3.Institute of Physical ChemistryJohannes Gutenberg UniversityMainzGermany
  4. 4.Max Planck Institute for Polymer ResearchMainzGermany

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