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Peptide Fragmentation Products in Mass Spectrometry Probed by Infrared Spectroscopy

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Gas-Phase IR Spectroscopy and Structure of Biological Molecules

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 364))

Abstract

Vibrational spectroscopy offers detailed insights, by virtue of diagnostic infrared bands, into the chemical structures and moieties which are formed during peptide fragmentation inside mass spectrometers. Over the past few years, IRMPD spectroscopy has led to a greatly improved understanding of the chemistry that takes place during collision-induced dissociation (CID) of protonated peptides. For instance, the rearrangement chemistry of b- and a-type ions, which is relevant in sequence scrambling pathways, has been directly confirmed with the technique. In this chapter, we provide a brief background on peptide fragmentation chemistry, and give an overview of areas where vibrational spectroscopy has been successfully implemented, such as CID of protonated and de-protonated peptides. We also discuss the potential of the technique for elucidating lesser-studied radical dissociation processes, such as electron capture dissociation (ECD), electron transfer dissociation (ETD), and laser photodissociation.

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Acknowledgements

Some of this work was supported by the U.S. National Science Foundation under grant number CHE-0845450. Student travel support to the Netherlands for measurements with the free electron laser FELIX was provided by the NSF-PIRE (grant number OISE-0730072). This material is based upon work supported by the National Science Foundation Graduate Research Fellowship (A.P.) under Grant No. (DGE-1315138).

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Authors and Affiliations

  1. Department of Chemistry, University of Florida, Buckman Drive, 117200, Gainesville, FL, 32611, USA

    Amanda L. Patrick & Nicolas C. Polfer

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  1. Amanda L. Patrick
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  2. Nicolas C. Polfer
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Correspondence to Nicolas C. Polfer .

Editor information

Editors and Affiliations

  1. Molecular and Biophysics, FELIX Laboratory, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands

    Anouk M. Rijs

  2. Molecular Structure and Dynamics, FELIX Laboratory, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands

    Jos Oomens

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Patrick, A.L., Polfer, N.C. (2014). Peptide Fragmentation Products in Mass Spectrometry Probed by Infrared Spectroscopy. In: Rijs, A., Oomens, J. (eds) Gas-Phase IR Spectroscopy and Structure of Biological Molecules. Topics in Current Chemistry, vol 364. Springer, Cham. https://doi.org/10.1007/128_2014_576

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