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Differentiation of Enantiomers by Capillary Electrophoresis

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Differentiation of Enantiomers I

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 340))

Abstract

Capillary electrophoresis (CE) has matured to one of the major liquid phase enantiodifferentiation techniques since the first report in 1985. This can be primarily attributed to the flexibility as well as the various modes available including electrokinetic chromatography (EKC), micellar electrokinetic chromatography (MEKC), and microemulsion electrokinetic chromatography (MEEKC). In contrast to chromatographic techniques, the chiral selector is mobile in the background electrolyte. Furthermore, a large variety of chiral selectors are available that can be easily combined in the same separation system. In addition, the migration order of the enantiomers can be adjusted by a number of approaches. In CE enantiodifferentiations the separation principle is comparable to chromatography while the principle of the movement of the analytes in the capillary is based on electrophoretic phenomena. The present chapter will focus on mechanistic aspects of CE enantioseparations including enantiomer migration order and the current understanding of selector–selectand structures. Selected examples of the basic enantioseparation modes EKC, MEKC, and MEEKC will be discussed.

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Abbreviations

CD:

Cyclodextrin

Dns:

Dansyl

EKC:

Electrokinetic chromatography

EOF:

Electroosmotic flow

id:

Inner diameter

MEKC:

Micellar electrokinetic chromatography

MEEKC:

Microemulsion electrokinetic chromatography

(+)-18C6H4:

(+)-(18-Crown-6)-2,3,11,12-tetracarboxylic acid

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Authors and Affiliations

  1. Department of Pharmaceutical/Medicinal Chemistry, Friedrich Schiller University Jena, Philosophenweg 14, 07743, Jena, Germany

    Gerhard K. E. Scriba

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  1. Gerhard K. E. Scriba
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Correspondence to Gerhard K. E. Scriba .

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  1. Institute of Organic Chemistry, University of Tübingen, Tübingen, Germany

    Volker Schurig

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Scriba, G.K.E. (2013). Differentiation of Enantiomers by Capillary Electrophoresis. In: Schurig, V. (eds) Differentiation of Enantiomers I. Topics in Current Chemistry, vol 340. Springer, Cham. https://doi.org/10.1007/128_2013_438

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