Abstract
Clinical biomarker discovery, verification, and validation are facilitated by the latest technological advances in mass spectrometry. It is now possible to analyze simultaneously group of tens or hundreds of biomarkers in a blood sample using multiple reaction monitoring (MRM), a tandem mass spectrometric method. However, these newly-developed methods face new challenges, including standardization, calibration, and the determination of analytical and biological variation. Here we illustrate the background, pre-analytical sample preparation, and biomarker assay development using an MRM-mass spectrometric method. In addition, special attention is given to future standardization methods to enable widespread use of the technology.
Keywords
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- APO4:
-
Apolipoprotein A4
- AUC:
-
Area under the curve
- CE:
-
Collision energy
- CID:
-
Collision-induced dissociation
- CV:
-
Coefficient of variation
- CVD:
-
Cardiovascular disease
- DDA:
-
Data-dependent acquisition
- EDRN:
-
Early Detection Research Network
- EDTA:
-
Ethylenediamine tetraacetic acid
- ESI:
-
Electrospray ionization
- HUPO:
-
Human Proteome Organization
- iMALDI:
-
Immuno-matrix-assisted laser-desorption/ionization
- iTRAQ™:
-
Isobaric tag for relative and absolute quantitation
- LC:
-
Liquid chromatography
- LOD:
-
Limit of detection
- LOQ:
-
Limit of quantitation
- MALDI:
-
Matrix-assisted laser-desorption/ionization
- MMP8:
-
Matrix metalloproteinase-8
- MRM:
-
Multiple reaction monitoring
- MS/MS:
-
Tandem mass spectrometry
- PSA:
-
Prostate specific antigen
- Q1 (Q2, Q3):
-
Quadrupole 1 (quadrupole 2, quadrupole 3)
- QUAD:
-
The Quantitative Assay Database
- ROC:
-
Receiver operator curve (or receiver operator characteristic)
- SIS peptides:
-
Stable-isotope-labeled internal standard peptides
- SISCAPA:
-
Stable isotope standards and capture by anti-peptide antibodies
- SRM:
-
Selected reaction monitoring
- UHPLC:
-
Ultrahigh-performance liquid chromatography
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Parker, C.E. et al. (2012). Mass Spectrometry in High-Throughput Clinical Biomarker Assays: Multiple Reaction Monitoring. In: L.S. Tang, N., Poon, T. (eds) Chemical Diagnostics. Topics in Current Chemistry, vol 336. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2012_353
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DOI: https://doi.org/10.1007/128_2012_353
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