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Prion Seeded Conversion and Amplification Assays

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Prion Proteins

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 305))

Abstract

The conversion of the normal prion protein (PrPC) into its misfolded, aggregation-prone and infectious (prion) isoform is central to the progression of transmissible spongiform encephalopathies (TSEs) or prion diseases. Since the initial development of a cell free PrP conversion reaction, striking progress has been made in the development of much more continuous prion-induced conversion and amplification reactions. These studies have provided major insights into the molecular underpinnings of prion propagation and enabled the development of ultra-sensitive tests for prions and prion disease diagnosis. This chapter will provide an overview of such reactions and the practical and fundamental consequences of their development.

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Acknowledgements

We thank Dr. Jason M. Wilham for designing the figures and Anita Mora for graphics assistance.

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Authors and Affiliations

  1. Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, 59840, USA

    Christina D. Orrú & Byron Caughey

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  1. Christina D. Orrú
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  2. Byron Caughey
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Correspondence to Byron Caughey .

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Editors and Affiliations

  1. Adolf-Butenandt-Institute, Abt. Biochemie, Universität München, Schillerstr. 44, München, 80336, Germany

    Jörg Tatzelt

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Orrú, C.D., Caughey, B. (2011). Prion Seeded Conversion and Amplification Assays. In: Tatzelt, J. (eds) Prion Proteins. Topics in Current Chemistry, vol 305. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_184

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