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Periplasmic Binding Proteins in Biosensing Applications

  • Felix S. Grünewald
Chapter
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Part of the Bioanalytical Reviews book series (BIOREV, volume 1)

Abstract

Periplasmic binding proteins (PBPs) of gram-negative bacteria have been widely used as recognition elements for the development of biosensors for small molecule analytes owing to their intrinsically high selectivity and affinity towards their cognate ligands. Analyte binding is accompanied by a large hinge motion that can readily be transduced to a detectable signal. While fundamental work demonstrating the versatility of PBPs as scaffolds for biosensors dates back to the 1990s, recent years have seen more subtle improvements in detection strategies. Measurement of cellular metabolites with PBP-based biosensors has allowed significant contributions to basic research, and a first functional sensor for continuous blood glucose monitoring with glucose-binding protein as biological recognition element was tested in preclinical trials. In this chapter, strategies and applications of biosensors using PBPs as specifiers will be reviewed.

Keywords

Periplasmic binding proteins Affinity based biosensors Solvatochromism Fluorescence biosensors 

Abbreviations

[Ru(bpy)3]2+

Ruthenium tris(2,2′-bipyridine)dichloride

5-IAF

5-Iodoacetamidofluorescein

ABP

Allose-binding protein

Acrylodan

6-Acryloyl-2-(dimethylamino)naphthalene

AF488

Alexa Fluor 488

AF680

Alexa Fluor 680

AF750

Alexa Fluor 750

ANS

2-(4′-Iodoacetamidoanilino)naphtalene-6-sulphonic acid

BADAN

2-Bromo-1-[6-(dimethylamino)-2-naphthalenyl]-ethanone

BLA

β-Lactamase

BP

Binding protein

BRET

Bioluminescence resonance energy transfer

cpGFP

Circularly permuted GFP

ECFP

Enhanced cyan fluorescent protein

EDC

1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide

EYFP

Enhanced yellow fluorescent protein

FLIP

Fluorescent indicator proteins

FP

Fluorescent protein

FRET

Förster resonance energy transfer

GBP

Glucose-/galactose-binding protein

GFP

Green fluorescent protein

GlnBP

Glutamine-binding protein

IAEDANS

5-(Iodoacetamidoethyl)aminonaphthalene-1-sulfonic acid

IANBD

4-[N-(2-(iodoacetoxy)ethyl)-N-methylamino]-7-nitrobenz-2-oxa-1,3-diazole

Kd

Dissociation constant

LIVBP

Branched-chain (leucine, isoleucine, valine) amino acid-binding protein

MBP

Maltose-binding protein

MDCC

N-[2-(l-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide

NBD

7-Nitrobenz-2-oxa-1,3-diazole

NHS

N-Hydroxysuccinimide

Ni-NTA

Nickel nitrilotriacetic acid

NIR

Near infrared

NMR

Nuclear magnetic resonance

PBP

Periplasmic binding protein

PEG

Polyethylene glycol

PEGDMA:MAA

PEG-dimethacrylate/methacrylic acid copolymer

PRE

Paramagnetic resonance enhancement

PDB

Protein Data Bank

RBP

Ribose-binding protein

RR

Rhodamine red

S/N

Signal-to-noise

SWV

Squarewave voltammetry

TMR

Tetramethyl rhodamine

ΔF

Fluorescence intensity change

τ

Fluorescence lifetime

Notes

Acknowledgments

I would like to thank Stacy DuVall for helpful discussions on electrochemical topics and critical reading of the draft. Thomas Meier is acknowledged for valuable suggestions to the draft. This work was promoted by the European Union under grant agreement number 264772 (ITN CHEBANA).

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Copyright information

© Springer International Publishing Switzerland 2013

Authors and Affiliations

  • Felix S. Grünewald
    • 1
    • 2
  1. 1.Department of Chemistry DevelopmentRoche Diabetes Care, Roche Diagnostics GmbHMannheimGermany
  2. 2.Department of Biotechnology DevelopmentRoche Professional Diagnostics, Roche Diagnostics GmbHPenzbergGermany

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