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Periplasmic Binding Proteins in Biosensing Applications

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Advances in Chemical Bioanalysis

Part of the book series: Bioanalytical Reviews ((BIOREV,volume 1))

Abstract

Periplasmic binding proteins (PBPs) of gram-negative bacteria have been widely used as recognition elements for the development of biosensors for small molecule analytes owing to their intrinsically high selectivity and affinity towards their cognate ligands. Analyte binding is accompanied by a large hinge motion that can readily be transduced to a detectable signal. While fundamental work demonstrating the versatility of PBPs as scaffolds for biosensors dates back to the 1990s, recent years have seen more subtle improvements in detection strategies. Measurement of cellular metabolites with PBP-based biosensors has allowed significant contributions to basic research, and a first functional sensor for continuous blood glucose monitoring with glucose-binding protein as biological recognition element was tested in preclinical trials. In this chapter, strategies and applications of biosensors using PBPs as specifiers will be reviewed.

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Abbreviations

[Ru(bpy)3]2+ :

Ruthenium tris(2,2′-bipyridine)dichloride

5-IAF:

5-Iodoacetamidofluorescein

ABP:

Allose-binding protein

Acrylodan:

6-Acryloyl-2-(dimethylamino)naphthalene

AF488:

Alexa Fluor 488

AF680:

Alexa Fluor 680

AF750:

Alexa Fluor 750

ANS:

2-(4′-Iodoacetamidoanilino)naphtalene-6-sulphonic acid

BADAN:

2-Bromo-1-[6-(dimethylamino)-2-naphthalenyl]-ethanone

BLA:

β-Lactamase

BP:

Binding protein

BRET:

Bioluminescence resonance energy transfer

cpGFP:

Circularly permuted GFP

ECFP:

Enhanced cyan fluorescent protein

EDC:

1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide

EYFP:

Enhanced yellow fluorescent protein

FLIP:

Fluorescent indicator proteins

FP:

Fluorescent protein

FRET:

Förster resonance energy transfer

GBP:

Glucose-/galactose-binding protein

GFP:

Green fluorescent protein

GlnBP:

Glutamine-binding protein

IAEDANS:

5-(Iodoacetamidoethyl)aminonaphthalene-1-sulfonic acid

IANBD:

4-[N-(2-(iodoacetoxy)ethyl)-N-methylamino]-7-nitrobenz-2-oxa-1,3-diazole

K d :

Dissociation constant

LIVBP:

Branched-chain (leucine, isoleucine, valine) amino acid-binding protein

MBP:

Maltose-binding protein

MDCC:

N-[2-(l-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide

NBD:

7-Nitrobenz-2-oxa-1,3-diazole

NHS:

N-Hydroxysuccinimide

Ni-NTA:

Nickel nitrilotriacetic acid

NIR:

Near infrared

NMR:

Nuclear magnetic resonance

PBP:

Periplasmic binding protein

PEG:

Polyethylene glycol

PEGDMA:MAA:

PEG-dimethacrylate/methacrylic acid copolymer

PRE:

Paramagnetic resonance enhancement

PDB:

Protein Data Bank

RBP:

Ribose-binding protein

RR:

Rhodamine red

S/N:

Signal-to-noise

SWV:

Squarewave voltammetry

TMR:

Tetramethyl rhodamine

ΔF:

Fluorescence intensity change

τ :

Fluorescence lifetime

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Acknowledgments

I would like to thank Stacy DuVall for helpful discussions on electrochemical topics and critical reading of the draft. Thomas Meier is acknowledged for valuable suggestions to the draft. This work was promoted by the European Union under grant agreement number 264772 (ITN CHEBANA).

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Authors and Affiliations

  1. Department of Chemistry Development, Roche Diabetes Care, Roche Diagnostics GmbH, Sandhofer Str. 116, 68305, Mannheim, Germany

    Felix S. Grünewald

  2. Department of Biotechnology Development, Roche Professional Diagnostics, Roche Diagnostics GmbH, Nonnenwald 2, 82377, Penzberg, Germany

    Felix S. Grünewald

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  1. Felix S. Grünewald
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  1. Institute of Analytical Chemistry, University of Regensburg, Regensburg, Germany

    Frank-Michael Matysik

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Grünewald, F.S. (2013). Periplasmic Binding Proteins in Biosensing Applications. In: Matysik, FM. (eds) Advances in Chemical Bioanalysis. Bioanalytical Reviews, vol 1. Springer, Cham. https://doi.org/10.1007/11663_2013_7

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