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Hyphenation of Electrochemistry with Mass Spectrometry for Bioanalytical Studies

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Advances in Chemical Bioanalysis

Part of the book series: Bioanalytical Reviews ((BIOREV,volume 1))

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Abstract

Hyphenation of electrochemistry (EC) and mass spectrometry (MS) is a growing research field with particular importance for bioanalytical applications. It opens the way for studying reaction mechanisms and metabolic pathways of biological compounds and drugs. Electrochemical conversion of sample molecules prior to MS analysis gives rise to short-lived intermediates and products naturally occurring in biological systems, which leads to better understanding of physiological processes. Numerous interesting and attractive studies in this field have been published so far demonstrating potential of EC–MS coupling. The combination with separation system such as liquid chromatography or capillary electrophoresis widens the scope of application providing additional information about compounds of interest. The combination of EC with liquid chromatography has been the most frequently used hyphenated system due to the simplicity of coupling to mass spectrometric detection. In terms of bioanalytical applications capillary electrophoresis offers some advantages and is a complementary technique to liquid chromatography. This review summarizes recent developments in this field from both instrumental and application perspectives. A rather new approach of coupling electrochemistry–capillary electrophoresis–mass spectrometry and its potential for bioanalysis is presented.

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Abbreviations

APAP:

Acetaminophen

AQ:

Amodiaquine

Arg:

Arginine

BDD:

Boron-doped diamond

CE:

Capillary electrophoresis

CEM:

Chain ejection model

CLZ:

Clozapine

CRM:

Charge residue model

DEMS:

Differential electrochemical mass spectrometry

DESI:

Desorption electrospray ionization

DNA:

Deoxyribonucleic acid

EAI:

Electrochemically assisted injection

EC:

Electrochemistry

ESI:

Electrospray ionization

FTICR:

Fourier transform ion cyclotron resonance

GAL:

Galantamine

GMP:

Guanosine monophosphate

GSH:

Glutathione

ICP–MS:

Inductively coupled plasma mass spectrometry

IDA:

Interdigitated array

IEM:

Ion evaporation model

IR:

Infrared

LC:

Liquid chromatography

LYC:

Lycorine

MALDI:

Matrix-assisted laser desorption/ionization

MEKC:

Micellar electrokinetic chromatography

MS:

Mass spectrometry

NACE:

Nonaqueous capillary electrophoresis

NMR:

Nuclear magnetic resonance

RNA:

Ribonucleic acid

SECM:

Scanning electrochemical microscopy

SPE:

Screen-printed electrode

TCC:

Triclocarban

TOF:

Time-of-flight

Trp:

Tryptophan

Tyr:

Tyrosine

β-LGA:

β-lactoglobulin

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Acknowledgments

This work was supported by the European Union under Grant Agreement number 264772 (ITN CHEBANA).

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  1. Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040, Regensburg, Germany

    Marija Cindric & Frank‐Michael Matysik

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  1. Marija Cindric
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  2. Frank‐Michael Matysik
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Correspondence to Frank‐Michael Matysik .

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  1. Institute of Analytical Chemistry, University of Regensburg, Regensburg, Germany

    Frank-Michael Matysik

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Cindric, M., Matysik, F. (2013). Hyphenation of Electrochemistry with Mass Spectrometry for Bioanalytical Studies. In: Matysik, FM. (eds) Advances in Chemical Bioanalysis. Bioanalytical Reviews, vol 1. Springer, Cham. https://doi.org/10.1007/11663_2013_6

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