The profile of profilins

  • B. M. JockuschEmail author
  • K. Murk
  • M. Rothkegel
Part of the Reviews of Physiology, Biochemistry and Pharmacology book series (REVIEWS, volume 159)


Profilins are small proteins involved in actin dynamics. In accordance with this function, they are found in all eukaryotes and are structurally highly conserved. However, their precise role in regulating actin-related functions is just beginning to emerge. This article recapitulates the wealth of information on structure, expression and functions accumulated on profilins from many different organisms in the 30 years after their discovery as actin-binding proteins. Emphasis is given to their interaction with a plethora of many different ligands in the cytoplasm as well as in the nucleus, which is considered the basis for their various activities and the significance of the tissue-specific expression of profilin isoforms.


Actin Filament Actin Polymerization Actin Dynamic Bovine Oocyte Spine Head 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This article relies on a wealth of data from many different laboratories. We have cited the appropriate original publications wherever possible, but want to apologize to all authors whose reports could not be cited because of space limitations. Our own research referred to here was supported by the “Deutsche Forschungsgemeinschaft,” the “Deutsche Krebshilfe/Mildred Scheel Stiftung” and the “Fonds der Deutschen Chemischen Industrie.” The neuronal cells used in Fig. 2 were provided by K. Michaelsen (Braunschweig).


  1. Ackermann M, Matus A (2003) Activity-induced targeting of profilin and stabilization of dendritic spine morphology. Nat Neurosci 6:1194–1200PubMedGoogle Scholar
  2. Arasada R, Gloss A, Tunggal B, Joseph JM, Rieger D, et al (2007) Profilin isoforms in Dictyostelium discoideum. Biochim Biophys Acta 1773:631–641PubMedGoogle Scholar
  3. Baatout S (1996) Profilin: an update. Eur J Clin Chem Clin Biochem 34:575–577PubMedGoogle Scholar
  4. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, et al (2000) The Protein Data Bank. Nucleic Acids Res 28:235–242PubMedGoogle Scholar
  5. Birbach A, Verkuyl JM, Matus A (2006) Reversible, activity-dependent targeting of profilin to neuronal nuclei. Exp Cell Res 312:2279–2287PubMedGoogle Scholar
  6. Blasco R, Cole NB, Moss B (1991) Sequence analysis, expression, and deletion of a vaccinia virus gene encoding a homolog of profilin, a eukaryotic actin-binding protein. J Virol 65:4598–4608PubMedGoogle Scholar
  7. Blessing CA, Ugrinova GT, Goodson HV (2004) Actin and ARPs: action in the nucleus. Trends Cell Biol 14:435–442PubMedGoogle Scholar
  8. Boukhelifa M, Moza M, Johansson T, Rachlin A, Parast M, et al (2006) The proline-rich protein palladin is a binding partner for profilin. Febs J 273:26–33PubMedGoogle Scholar
  9. Boyl PP, Di Nardo A, Mulle C, Sassoè-Pognetto M, Panzanelli P, Mele A, Kneussel M, Costantini V, Perlas E, Massimi M, Giustetto M, Rivera HV, Witke W (2007) Profilin2 contributes to synaptic vesicle exocytosis, neuronal excitability and novelty-seeking behavior. EMBO J 26:2991–3002Google Scholar
  10. Braun A, Aszodi A, Hellebrand H, Berna A, Fassler R, Brandau O (2002) Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis. Gene 283:219–225PubMedGoogle Scholar
  11. Breiteneder H, Ebner C (2001) Atopic allergens of plant foods. Curr Opin Allergy Clin Immunol 1:261–267PubMedGoogle Scholar
  12. Buss F, Temm-Grove C, Henning S, Jockusch BM (1992) Distribution of profilin in fibroblasts correlates with the presence of highly dynamic actin filaments. Cell Motil Cytoskeleton 22:51–61PubMedGoogle Scholar
  13. Carlsson L, Nystrom L, Sundkvist I, Markey F, Lindberg U (1976) Profilin, a low-molecular weight protein controlling actin polymerisability. In: Perry SV, Marzella A, Adelstein RS (eds) Contractile systems in non muscle tissues. North Holland Publishing, Amsterdam, pp 39–49Google Scholar
  14. Carlsson L, Nyström NE, Sundkvist I, Markey F, Lindberg U (1977) Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells. J Biochem Mol Biol 115:465–483Google Scholar
  15. Cedergren-Zeppezauer ES, Goonesekere NC, Rozycki MD, Myslik JC, Dauter Z, et al (1994) Crystallization and structure determination of bovine profilin at 2.0 A resolution. J Mol Biol 240:459–475PubMedGoogle Scholar
  16. Chang F, Drubin D, Nurse P (1997) cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin. J Cell Biol 137:169–82PubMedGoogle Scholar
  17. Cooley L, Verheyen E, Ayers K (1992) chickadee encodes a profilin required for intercellular cytoplasm transport during Drosophila oogenesis. Cell 69:173–184PubMedGoogle Scholar
  18. Da Silva JS, Medina M, Zuliani C, Di Nardo A, Witke W, Dotti CG (2003) RhoA/ROCK regulation of neuritogenesis via profilin IIa-mediated control of actin stability. J Cell Biol 162:1267–1279PubMedGoogle Scholar
  19. Deeks MJ, Hussey PJ (2003) Arp2/3 and 'the shape of things to come'. Curr Opin Plant Biol 6:561–567PubMedGoogle Scholar
  20. Deeks MJ, Hussey PJ, Davies B (2002) Formins: intermediates in signal-transduction cascades that affect cytoskeletal reorganization. Trends Plant Sci 7:492–498PubMedGoogle Scholar
  21. Di Nardo A, Gareus R, Kwiatkowski D, Witke W (2000) Alternative splicing of the mouse profilin II gene generates functionally different profilin isoforms. J Cell Sci 113:3795–3803PubMedGoogle Scholar
  22. Ding Z, Lambrechts A, Parepally M, Roy P (2006) Silencing profilin-1 inhibits endothelial cell proliferation, migration and cord morphogenesis. J Cell Sci 119:4127–4137PubMedGoogle Scholar
  23. Domke T, Federau T, Schluter K, Giehl K, Valenta R, et al (1997) Birch pollen profilin: structural organization and interaction with poly-(L-proline) peptides as revealed by NMR. FEBS Lett 411:291–295PubMedGoogle Scholar
  24. Dong J, Radau B, Otto A, Muller E, Lindschau C, Westermann P (2000) Profilin I attached to the Golgi is required for the formation of constitutive transport vesicles at the trans-Golgi network. Biochim Biophys Acta 1497:253–260PubMedGoogle Scholar
  25. Evangelista M, Blundell K, Longtine MS, Chow CJ, Adames N, et al (1997) Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis. Science 276:118–22PubMedGoogle Scholar
  26. Faix J, Grosse R (2006) Staying in shape with formins. Dev Cell 10:693–706PubMedGoogle Scholar
  27. Fedorov AA, Magnus KA, Graupe MH, Lattman EE, Pollard TD, Almo SC (1994) X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates. Proc Natl Acad Sci USA 91:8636–8640PubMedGoogle Scholar
  28. Fedorov AA, Ball T, Mahoney NM, Valenta R, Almo SC (1997) The molecular basis for allergen cross-reactivity: crystal structure and IgE-epitope mapping of birch pollen profilin. Structure 5:33–45PubMedGoogle Scholar
  29. Feng G, Tintrup H, Kirsch J, Nichol MC, Kuhse J, et al (1998) Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science 282:1321–1324PubMedGoogle Scholar
  30. Finger FP, Novick P (1997) Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell 8:647–662PubMedGoogle Scholar
  31. Gareus R, Di Nardo A, Rybin V, Witke W (2006) Mouse profilin 2 regulates endocytosis and competes with SH3 ligand binding to dynamin 1. J Biol Chem 281:2803–2811PubMedGoogle Scholar
  32. Garner CC, Kindler S, Gundelfinger ED (2000) Molecular determinants of presynaptic active zones. Curr Opin Neurobiol 10:321–327PubMedGoogle Scholar
  33. Gertler FB, Niebuhr K, Reinhard M, Wehland J, Soriano P (1996) Mena, a relative of VASP and Drosophila enabled, is implicated in the control of microfilament dynamics. Cell 87:227–239PubMedGoogle Scholar
  34. Gibbon BC, Zonia LE, Kovar DR, Hussey PJ, Staiger CJ (1998) Pollen profilin function depends on interaction with proline-rich motifs. Plant Cell 10:981–993PubMedGoogle Scholar
  35. Giehl K, Valenta R, Rothkegel M, Ronsiek M, Mannherz HG, Jockusch BM (1994) Interaction of plant profilin with mammalian actin. Eur J Biochem 226:681–689PubMedGoogle Scholar
  36. Giesemann T, Rathke-Hartlieb S, Rothkegel M, Bartsch JW, Buchmeier S, et al (1999) A role for polyproline motifs in the spinal muscular atrophy protein SMN: profilins bind to and colocalize with SMN in nuclear gems. J Biol Chem 274:37908–37914PubMedGoogle Scholar
  37. Giesemann T, Schwarz G, Nawrotzki R, Berhörster K, Rothkegel M, et al (2003) Complex formation between the postsynaptic scaffolding protein gephyrin, profilin and Mena: a possible link to the microfilament system. J Neurosci 23:8330–8339PubMedGoogle Scholar
  38. Goldschmidt-Clermont PJ, Kim JW, Machesky LM, Rhee SG, Pollard TD (1991) Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation. Science 251:1231–1233PubMedGoogle Scholar
  39. Grummt I (2006) Actin and myosin as transcription factors. Curr Opin Genet Dev 16:191–196PubMedGoogle Scholar
  40. Gutsche-Perelroizen I, Lepault J, Ott A, Carlier MF (1999) Filament assembly from profilin-actin. J Biol Chem 274:6234–6243PubMedGoogle Scholar
  41. Haarer BK, Brown SS (1990) Structure and function of profilin. Cell Motil Cytoskeleton 17:71–74PubMedGoogle Scholar
  42. Harbeck B, Hüttelmaier S, Schlüter K, Jockusch BM, Illenberger S (2000) Phosphorylation of the vasodilator-stimulated phosphoprotein regulates its interaction with actin. J Biol Chem 275:30817–30825PubMedGoogle Scholar
  43. Haugwitz M, Noegel AA, Rieger D, Lottspeich F, Schleicher M (1991) Dictyostelium discoideum contains two profilin isoforms that differ in structure and function. J Cell Sci 100:481–489PubMedGoogle Scholar
  44. Haugwitz M, Noegel AA, Karakesisoglou J, Schleicher M (1994) Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development. Cell 79:303–314PubMedGoogle Scholar
  45. Higgins HN (2005) Formin proteins: a domain-based approach. Trends Biochem Sci 30:342–353Google Scholar
  46. Honoré B, Madsen P, Andersen AH, Leffers H (1993) Cloning and expression of a novel human profilin variant, profilin II. Febs Lett 330:151–155PubMedGoogle Scholar
  47. Hu E, Chen Z, Fredrickson T, Zhu Y (2001) Molecular cloning and characterization of profilin-3: a novel cytoskeleton-associated gene expressed in rat kidney and testes. Exp Nephrol 9:265–274PubMedGoogle Scholar
  48. Huang SR, McDowell JM, Weise MJ, Meagher RB (1996) The Arabidopsis profilin gene family—Evidence for an ancient split between constitutive and pollen-specific profilin genes. Plant Physiol 111:115–126PubMedGoogle Scholar
  49. Hüttelmaier S, Mayboroda O, Harbeck B, Jarchau T, Jockusch BM, Rüdiger M (1998) The interaction of the cell-contact proteins VASP and vinculin is regulated by phosphatidylinositol-4,5-bisphosphate. Curr Biol 8:479–488PubMedGoogle Scholar
  50. Hüttelmaier S, Harbeck B, Steffens O, Messerschmidt T, Illenberger S, Jockusch BM (1999) Characterization of the actin binding properties of the vasodilator-stimulated phosphoprotein VASP. FEBS Lett 451:68–74PubMedGoogle Scholar
  51. Hussey PJ, Ketelaar T, Deeks MJ (2006) Control of the actin cytoskeleton in plant cell growth. Annu Rev Plant Biol 57:109–125PubMedGoogle Scholar
  52. Janke J, Schluter K, Jandrig B, Theile M, Kolble K, et al (2000) Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1. J Exp Med 191:1675–1686PubMedGoogle Scholar
  53. Jockusch BM, Rothkegel M, Schwarz G (2004) Linking the synapse to the cytoskeleton: a breath-taking role for microfilaments. Neuroreport 15:1535–1538PubMedGoogle Scholar
  54. Jockusch BM, Schoenenberger CA, Stetefeld J, Aebi U (2006) Tracking down the different forms of nuclear actin. Trends Cell Biol 16:391–396PubMedGoogle Scholar
  55. Kandasamy MK, McKinney EC, Meagher RB (2002) Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52:22–32PubMedGoogle Scholar
  56. Karakesisoglou I, Schleicher M, Gibbon BC, Staiger CJ (1996) Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells. Cell Motil Cytoskeleton 34:36–47PubMedGoogle Scholar
  57. Kelleher JF, Atkinson SJ, Pollard TD (1995) Sequences, structural models, and cellular localization of the actin-related proteins Arp2 and Arp3 from Acanthamoeba. J Cell Biol 131:385–397PubMedGoogle Scholar
  58. Kim E, Waters SH, Hake LE, Hecht NB (1989) Identification and developmental expression of a smooth-muscle gamma-actin in postmeiotic male germ cells of mice. Mol Cell Biol 9:1875–1881PubMedGoogle Scholar
  59. Kovar DR, Drobak BK, Staiger CJ (2000) Maize profilin isoforms are functionally distinct. Plant Cell 12:583–598PubMedGoogle Scholar
  60. Kovar DR, Harris ES, Mahaffy R, Higgs HN, Pollard TD (2006) Control of the assembly of ATP- and ADP-actin by formins and profilin. Cell 124:423–435PubMedGoogle Scholar
  61. Krause M, Dent EW, Bear JE, Loureiro JJ, Gertler FB (2003) Ena/VASP proteins: regulators of the actin cytoskeleton and cell migration. Annu Rev Cell Dev Biol 19:541–564PubMedGoogle Scholar
  62. Krebs A, Rothkegel M, Klar M, Jockusch BM (2001) Characterization of functional domains of mDia1, a link between the small GTPase Rho and the actin cytoskeleton. J Cell Sci 114:3663–3672PubMedGoogle Scholar
  63. Kruchten AE, McNiven MA (2006) Dynamin as a mover and pincher during cell migration and invasion. J Cell Sci 119:1683–1690PubMedGoogle Scholar
  64. Lafuente EM, van Puijenbroek AA, Krause M, Carman CV, Freeman GJ, et al (2004) RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Dev Cell 7:585–595PubMedGoogle Scholar
  65. Lambrechts A, Verschelde JL, Jonckheere V, Goethals M, Vandekerckhove J, Ampe C (1997) The mammalian profilin isoforms display complementary affinities for PIP2 and proline-rich sequences. EMBO J 16:484–494PubMedGoogle Scholar
  66. Lambrechts A, Jonckheere V, Dewitte D, Vandekerckhove J, Ampe C (2002) Mutational analysis of human profilin I reveals a second PIP2 binding site neighbouring the poly(L-proline)binding site. BMC Biochem 3:12PubMedGoogle Scholar
  67. Lambrechts A, Jonckheere V, Peleman C, Polet D, De Vos W, et al (2006) Profilin-I-ligand interactions influence various aspects of neuronal differentiation. J Cell Sci 119:1570–1578PubMedGoogle Scholar
  68. Lamprecht R, Farb CR, Rodrigues SM, LeDoux JE (2006) Fear conditioning drives profilin into amygdala dendritic spines. Nat Neurosci 9:481–483PubMedGoogle Scholar
  69. Lassing I, Lindberg U (1985) Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin. Nature 314:472–474PubMedGoogle Scholar
  70. Lederer M, Jockusch BM, Rothkegel M (2005) Profilin regulates the activity of p42POP, a novel Myb-related transcription factor. J Cell Sci 118:331–341PubMedGoogle Scholar
  71. Li F, Higgs HN (2003) The mouse Formin mDia1 is a potent actin nucleation factor regulated by autoinhibition. Curr Biol 13:1335–1340PubMedGoogle Scholar
  72. Lu PJ, Shieh WR, Rhee SG, Yin HL, Chen CS (1996) Lipid products of phosphoinositide 3-kinase bind human profilin with high affinity. Biochemistry 35:14027–14034PubMedGoogle Scholar
  73. Machesky LM, Atkinson SJ, Ampe C, Vandekerckhove J, Pollard TD (1994a) Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J Cell Biol 127:107–115PubMedGoogle Scholar
  74. Machesky LM, Cole NB, Moss B, Pollard TD (1994b) Vaccinia virus expresses a novel profilin with a higher affinity for polyphosphoinositides than actin. Biochemistry 33:10815–10824PubMedGoogle Scholar
  75. Mahoney NM, Janmey PA, Almo SC (1997) Structure of the profilin-poly-l-proline complex involved in morphogenesis and cytoskeletal regulation. Nat Struct Biol 4:953–960PubMedGoogle Scholar
  76. Mammoto A, Sasaki T, Asakura T, Hotta I, Imamura H, et al (1998) Interactions of drebrin and gephyrin with profilin. Biochem Biophys Res Commun 243:86–89PubMedGoogle Scholar
  77. Manseau L, Calley J, Phan H (1996) Profilin is required for posterior patterning of the Drosophila oocyte. Development 122:2109–2116PubMedGoogle Scholar
  78. Mayboroda O, Schlüter K, Jockusch BM (1997) Differential colocalization of profilin with microfilaments in PtK2 cells. Cell Motil. Cell Motil Cytoskeleton 37:166–177PubMedGoogle Scholar
  79. McCollum D, Feoktistova A, Morphew M, Balasubramanian M, Gould KL (1996) The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin. EMBO J 15:6438–6446PubMedGoogle Scholar
  80. McDowell JM, Huang S, McKinney EC, An YQ, Meagher RB (1996) Structure and evolution of the actin gene family in Arabidopsis thaliana. Genetics 142:587–602PubMedGoogle Scholar
  81. McKenna ST, Vidali L, Hepler PK (2004) Profilin inhibits pollen tube growth through actin-binding, but not poly-l-proline-binding. Planta 218:906–915PubMedGoogle Scholar
  82. McWhorter ML, Monani UR, Burghes AH, Beattie CE (2003) Knockdown of the survival motor neuron (Smn) protein in zebrafish causes defects in motor axon outgrowth and pathfinding. J Cell Biol 162:919–931PubMedGoogle Scholar
  83. Michelot A, Guerin C, Huang S, Ingouff M, Richard S, et al (2005) The formin homology 1 domain modulates the actin nucleation and bundling activity of Arabidopsis FORMIN1. Plant Cell 17:2296–2313PubMedGoogle Scholar
  84. Miki H, Suetsugu S, Takenawa T (1998) WAVE, a novel WASP-family protein involved in actin reorganization induced by rac. EMBO J 17:6932–6941PubMedGoogle Scholar
  85. Minehardt TJ, Kollman PA, Cooke R, Pate E (2006) The open nucleotide pocket of the profilin/actin x-ray structure is unstable and closes in the absence of profilin. Biophys J 90:2445–2449PubMedGoogle Scholar
  86. Mourelatos Z, Abel L, Yong J, Kataoka N, Dreyfuss G (2001) SMN interacts with a novel family of hnRNP and spliceosomal proteins. EMBO J 20:5443–5452PubMedGoogle Scholar
  87. Muller J, Oma Y, Vallar L, Friederich E, Poch O, Winsor B (2005) Sequence and comparative genomic analysis of actin-related proteins. Mol Biol Cell 16:5736–5748PubMedGoogle Scholar
  88. Neuhoff H, Sassoe-Pognetto M, Panzanelli P, Maas C, Witke W, Kneussel M (2005) The actin-binding protein profilin I is localized at synaptic sites in an activity-regulated manner. Eur J Neurosci 21:15–25PubMedGoogle Scholar
  89. Obermann H, Raabe I, Balvers M, Brunswig B, Schulze W, Kirchhoff C (2005) Novel testis-expressed profilin IV associated with acrosome biogenesis and spermatid elongation. Mol Hum Reprod 11:53–64PubMedGoogle Scholar
  90. Ozaki K, Sugino H, Hasegawa T, Takahashi S, Hatano S (1983) Isolation and characterization of Physarum profilin. J Biochem (Tokyo) 93:295–298Google Scholar
  91. Palazzo AF, Cook TA, Alberts AS, Gundersen GG (2001) mDia mediates Rho-regulated formation and orientation of stable microtubules. Nat Cell Biol 3:723–729PubMedGoogle Scholar
  92. Peitsch WK, Bulkescher J, Spring H, Hofmann I, Goerdt S, Franke WW (2006) Dynamics of the actin-binding protein drebrin in motile cells and definition of a juxtanuclear drebrin-enriched zone. Exp Cell Res 312:2605–2618PubMedGoogle Scholar
  93. Perelroizen I, Carlier MF, Pantaloni D (1995) Binding of divalent cation and nucleotide to G-actin in the presence of profilin. J Biol Chem 270:1501–1508PubMedGoogle Scholar
  94. Polet D, Lambrechts A, Ono K, Mah A, Peelman F, et al (2006) Caenorhabditis elegans expresses three functional profilins in a tissue-specific manner. Cell Motil Cytoskeleton 63:14–28PubMedGoogle Scholar
  95. Polet D, Lambrechts A, Vandepoele K, Vandekerckhove J, Ampe C (2007) On the origin and evolution of vertebrate and viral profilins. FEBS Lett 581:211–217PubMedGoogle Scholar
  96. Pollard TD, Borisy GG (2003) Cellular motility driven by assembly and disassembly of actin filaments. Cell 113:549Google Scholar
  97. Pollard TD, Quirk S (1994) Profilins, ancient actin binding proteins with highly divergent primary structures. Soc Gen Physiol Ser 49:117–128PubMedGoogle Scholar
  98. Pruyne D, Evangelista M, Yang C, Bi E, Zigmond S, et al (2002) Role of formins in actin assembly: nucleation and barbed-end association. Science 297:612–615PubMedGoogle Scholar
  99. Radauer C, Willerroider M, Fuchs H, Hoffmann-Sommergruber K, Thalhamer J, et al (2006) Cross-reactive and species-specific immunoglobulin E epitopes of plant profilins: an experimental and structure-based analysis. Clin Exp Allergy 36:920–929PubMedGoogle Scholar
  100. Ramming M, Kins S, Werner N, Hermann A, Betz H, Kirsch J (2000) Diversity and phylogeny of gephyrin: tissue-specific splice variants, gene structure, and sequence similarities to molybdenum cofactor-synthesizing and cytoskeleton-associated proteins. Proc Natl Acad Sci USA 97:10266–10271PubMedGoogle Scholar
  101. Rawe VY, Payne C, Schatten G (2006) Profilin and actin-related proteins regulate microfilament dynamics during early mammalian embryogenesis. Hum Reprod 21:1143–1153PubMedGoogle Scholar
  102. Reichstein E, Korn ED (1979) Acanthamoeba profilin. A protein of low molecular weight from Acanpthamoeba castellanii that inhibits actin nucleation. J Biol Chem 254:6174–6179PubMedGoogle Scholar
  103. Reinhard M, Halbrugge M, Scheer U, Wiegand C, Jockusch BM, Walter U (1992) The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts. EMBO J 11:2063–2070PubMedGoogle Scholar
  104. Reinhard M, Giehl K, Abel K, Haffner C, Jarchau T, et al (1995) The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. EMBO J 14:1583–1589PubMedGoogle Scholar
  105. Reinhard M, Rudiger M, Jockusch BM, Walter U (1996) VASP interaction with vinculin: A recurring theme of interactions with proline-rich motifs. FEBS Lett 399:103–107PubMedGoogle Scholar
  106. Romero S, Le Clainche C, Didry D, Egile C, Pantaloni D, Carlier MF (2004) Formin is a processive motor that requires profilin to accelerate actin assembly and associated ATP hydrolysis. Cell 119:419–429PubMedGoogle Scholar
  107. Romero S, Didry D, Larquet E, Boisset N, Pantaloni D, Carlier MF (2007) How ATP hydrolysis controls filament assembly from profilin-actin: implication for formin processivity. J Biol Chem 282:8435–8445PubMedGoogle Scholar
  108. Rothkegel M, Mayboroda O, Rohde M, Wucherpfennig C, Valenta R, Jockusch BM (1996) Plant and animal profilins are functionally equivalent and stabilize microfilaments in living animal cells. J Cell Sci 109:83–90PubMedGoogle Scholar
  109. Rottner K, Behrendt B, Small JV, Wehland J (1999) VASP dynamics during lamellipodia protrusion. Nat Cell Biol 1:321–322PubMedGoogle Scholar
  110. Sankian M, Varasteh A, Pazouki N, Mahmoudi M (2005) Sequence homology: a poor predictive value for profilins cross-reactivity. Clin Mol Allergy 3:13PubMedGoogle Scholar
  111. Sathish K, Padma B, Munugalavadla V, Bhargavi V, Radhika KV, et al (2004) Phosphorylation of profilin regulates its interaction with actin and poly (L-proline). Cell Signal 16:589–596PubMedGoogle Scholar
  112. Sato A, Khadka DK, Liu W, Bharti R, Runnels LW, et al (2006) Profilin is an effector for Daam1 in non-canonical Wnt signaling and is required for vertebrate gastrulation. Development 133:4219–4231PubMedGoogle Scholar
  113. Schlüter K, Jockusch BM, Rothkegel M (1997) Profilins as regulators of actin dynamics. Biochim Biophys Acta 27:97–109Google Scholar
  114. Schlüter K, Schleicher M, Jockusch BM (1998) Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I. J Cell Sci 111:3261–3273PubMedGoogle Scholar
  115. Schmitz HP, Kaufmann A, Kohli M, Laissue PP, Philippsen P (2006) From function to shape: a novel role of a formin in morphogenesis of the fungus Ashbya gossypii. Mol Biol Cell 17:130–145PubMedGoogle Scholar
  116. Schutt CE, Myslik JC, Rozycki MD, Goonesekere NC, Lindberg U (1993) The structure of crystalline profilin-beta-actin. Nature 365:810–816PubMedGoogle Scholar
  117. Selden LA, Kinosian HJ, Estes JE, Gershman LC (1999) Impact of profilin on actin-bound nucleotide exchange and actin polymerization dynamics. Biochemistry 38:2769–2778PubMedGoogle Scholar
  118. Sharma A, Lambrechts A, Hao le T, Le TT, Sewry CA, et al (2005) A role for complexes of survival of motor neurons (SMN) protein with gemins and profilin in neurite-like cytoplasmic extensions of cultured nerve cells. Exp Cell Res 309:185–197PubMedGoogle Scholar
  119. Shirao T (1995) The roles of microfilament-associated proteins, drebrins, in brain morphogenesis: a review. J Biochem (Tokyo) 117:231–236Google Scholar
  120. Skare P, Karlsson R (2002) Evidence for two interaction regions for phosphatidylinositol (4,5)-bisphosphate on mammalian profilin I. FEBS Lett 522:119–124PubMedGoogle Scholar
  121. Skare P, Kreivi JP, Bergstrom A, Karlsson R (2003) Profilin I colocalizes with speckles and Cajal bodies: a possible role in pre-mRNA splicing. Exp Cell Res 286:12–21PubMedGoogle Scholar
  122. Solomaha E, Szeto FL, Yousef MA, Palfrey HC (2005) Kinetics of Src homology 3 domain association with the proline-rich domain of dynamins: specificity, occlusion, and the effects of phosphorylation. J Biol Chem 280:23147–23156PubMedGoogle Scholar
  123. Somboonwiwat K, Supungul P, Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A (2006) Differentially expressed genes in hemocytes of Vibrio harveyi-challenged shrimp Penaeus monodon. J Biochem Mol Biol 39:26–36PubMedGoogle Scholar
  124. Staiger CJ, Goodbody KC, Hussey PJ, Valenta R, Drobak BK, Lloyd CW (1993) The profilin multigene family of maize: differential expression of three isoforms. Plant J 4:631–641PubMedGoogle Scholar
  125. Stradal TE, Rottner K, Disanza A, Confalonieri S, Innocenti M, Scita G (2004) Regulation of actin dynamics by WASP and WAVE family proteins. Trends Cell Biol 14:303–311PubMedGoogle Scholar
  126. Stüven T, Hartmann E, Görlich D (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin-actin complexes. EMBO J 22:5928–5940PubMedGoogle Scholar
  127. Suetsugu S, Miki H, Takenawa T (1998) The essential role of profilin in the assembly of actin for microspike formation. EMBO J 17:6516–6926PubMedGoogle Scholar
  128. Takamori S, Holt M, Stenius K, Lemke EA, Gronborg M, et al (2006) Molecular anatomy of a trafficking organelle. Cell 127:831–846PubMedGoogle Scholar
  129. Tanaka H, Iguchi N, Egydio de Carvalho C, Tadokoro Y, Yomogida K, Nishimune Y (2003) Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially expressed in the cytoplasm and nucleus of mouse haploid germ cells. Biol Reprod 69:475–482PubMedGoogle Scholar
  130. Thorn KS, Christensen HEM, Shigeta Jr R, Huddler Jr D, Shalaby L, et al (1997) The crystal structure of a major allergen from plants. Structure 5:19–32PubMedGoogle Scholar
  131. Tilney LG, Bonder EM, Coluccio LM, Mooseker MS (1983) Actin from Thyone sperm assembles on only one end of an actin filament: a behavior regulated by profilin. J Cell Biol 97:112–124PubMedGoogle Scholar
  132. Valenta R, Duchene M, Pettenburger K, Sillaber C, Valent P, et al (1991) Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals. Science 253:557–560PubMedGoogle Scholar
  133. Valenta R, Duchene M, Ebner C, Valent P, Sillaber C, et al (1992) Profilins constitute a novel family of functional plant pan-allergens. J Exp Med 175:377–385PubMedGoogle Scholar
  134. Valenta R, Ferreira F, Grote M, Swoboda I, Vrtala S, et al (1993) Identification of profilin as an actin-binding protein in higher plants. J Biol Chem 268:22777–22781PubMedGoogle Scholar
  135. van Ree R, Voitenko V, van Leeuwen WA, Aalberse RC (1992) Profilin is a cross-reactive allergen in pollen and vegetable foods. Int Arch Allergy Immunol 98:97–104PubMedGoogle Scholar
  136. Walders-Harbeck B, Khaitlina SY, Hinssen H, Jockusch BM, Illenberger S (2002) The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS Lett 26564:1–6Google Scholar
  137. Wallar BJ, Alberts AS (2003) The formins: active scaffolds that remodel the cytoskeleton. Trends Cell Biol 13:435–446PubMedGoogle Scholar
  138. Wang X, Kibschull M, Laue MM, Lichte B, Petrasch-Parwez E, Kilimann MW (1999) Aczonin, a 550-kD putative scaffolding protein of presynaptic active zones, shares homology regions with Rim and Bassoon and binds profilin. J Cell Biol 147:151–162PubMedGoogle Scholar
  139. Watanabe N, Madaule P, Reid T, Ishizaki T, Watanabe G, et al (1997) p140mDia, a mammalian homologue of Drosophila diaphanous, is a target protein for rho small GTPases and is a ligand for profilin. EMBO J 16:3044–3056PubMedGoogle Scholar
  140. Wilkes DE, Otto JJ (2000) Molecular cloning of profilin from Tetrahymena thermophila. Gene 246:295–301PubMedGoogle Scholar
  141. Wilkes DE, Otto JJ (2003) Profilin functions in cytokinesis, nuclear positioning, and stomatogenesis in Tetrahymena thermophila. J Eukaryot Microbiol 50:252–262PubMedGoogle Scholar
  142. Witke W (2004) The role of profilin complexes in cell motility and other cellular processes. Trends Cell Biol 14:461–469PubMedGoogle Scholar
  143. Witke W, Podtelejnikov AV, Di Nardo A, Sutherland JD, Gurniak CB, et al (1998) In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly. EMBO J 17:967–976PubMedGoogle Scholar
  144. Witke W, Sutherland JD, Sharpe A, Arai M, Kwiatkowski DJ (2001) Profilin I is essential for cell survival and cell division in early mouse development. Proc Natl Acad Sci USA 98:3832–3836PubMedGoogle Scholar
  145. Wittenmayer N, Jandrig B, Rothkegel M, Schluter K, Arnold W, et al (2004) Tumor suppressor activity of profilin requires a functional actin binding site. Mol Biol Cell 15:1600–1608PubMedGoogle Scholar
  146. Wu X, Yoo Y, Okuhama NN, Tucker PW, Liu G, Guan JL (2006) Regulation of RNA-polymerase-II-dependent transcription by N-WASP and its nuclear-binding partners. Nat Cell Biol 8:756–763PubMedGoogle Scholar
  147. Yarmola EG, Bubb MR (2006) Profilin: emerging concepts and lingering misconceptions. Trends Biochem Sci 31:197–205PubMedGoogle Scholar
  148. Zhang H, Xing L, Rossoll W, Wichterle H, Singer RH, Bassell GJ (2006) Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons. J Neurosci 26:8622–8632PubMedGoogle Scholar
  149. Zhao W, Jiang C, Kroll TT, Huber PW (2001) A proline-rich protein binds to the localization element of Xenopus Vg1 mRNA and to ligands involved in actin polymerization. EMBO J 20:2315–2325PubMedGoogle Scholar
  150. Zhou F, Leder P, Martin SS (2006) Formin-1 protein associates with microtubules through a peptide domain encoded by exon-2. Exp Cell Res 312:1119–1126PubMedGoogle Scholar
  151. Zigmond SH (2004) Formin-induced nucleation of actin filaments. Curr Opin Cell Biol 16:99–105PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Cell Biology, Zoological InstituteTechnical University of BraunschweigBraunschweigGermany

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