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Synergizing 13C Metabolic Flux Analysis and Metabolic Engineering for Biochemical Production

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Synthetic Biology – Metabolic Engineering

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 162))

Abstract

Metabolic engineering of industrial microorganisms to produce chemicals, fuels, and drugs has attracted increasing interest as it provides an environment-friendly and renewable route that does not depend on depleting petroleum sources. However, the microbial metabolism is so complex that metabolic engineering efforts often have difficulty in achieving a satisfactory yield, titer, or productivity of the target chemical. To overcome this challenge, 13C Metabolic Flux Analysis (13C-MFA) has been developed to investigate rigorously the cell metabolism and quantify the carbon flux distribution in central metabolic pathways. In the past decade, 13C-MFA has been widely used in academic labs and the biotechnology industry to pinpoint the key issues related to microbial-based chemical production and to guide the development of the appropriate metabolic engineering strategies for improving the biochemical production. In this chapter we introduce the basics of 13C-MFA and illustrate how 13C-MFA has been applied to synergize with metabolic engineering to identify and tackle the rate-limiting steps in biochemical production.

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Acknowledgements

We thank Herbert Huttanus in Virginia Tech for improving the language of the chapter. This study was supported by start-up fund (#175323) from Virginia Tech and Junior Faculty Award from Institute for Critical Technology and Applied Science.

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  1. Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA

    Weihua Guo, Jiayuan Sheng & Xueyang Feng

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  1. Weihua Guo
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  2. Jiayuan Sheng
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Correspondence to Xueyang Feng .

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  1. Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana, Illinois, USA

    Huimin Zhao

  2. Institut für Bioprozess- und Biosystemtechnik, Technische Universitðt Hamburg-Harburg, Hamburg, Germany

    An-Ping Zeng

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Guo, W., Sheng, J., Feng, X. (2017). Synergizing 13C Metabolic Flux Analysis and Metabolic Engineering for Biochemical Production. In: Zhao, H., Zeng, AP. (eds) Synthetic Biology – Metabolic Engineering. Advances in Biochemical Engineering/Biotechnology, vol 162. Springer, Cham. https://doi.org/10.1007/10_2017_2

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